Show Order Info
SKU:MA1007
Clonality:Monoclonal
Clone Number:CB-955
Application:IHC-P, WB
Price: $200.00
Quantity:
Technical
Specs
Kit
Components
Gene
Information
Application
Data & Images
Reviews
Publications
FAQs

Overview

Product Name Anti-Calbindin-D Antibody (Monoclonal, CB-955)
Description Mouse IgG monoclonal antibody for Calbindin-D, calbindin 1, 28kDa (CALB1) detection. Tested with WB, IHC-P in Human;mouse;rat;bovine. No cross reactivity with other proteins.
Cite This Product Anti-Calbindin-D Antibody (Monoclonal, CB-955) CB-955 (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1007)
Replacement Item This antibody may replace the following items: sc-135666|sc-28285|sc-365360|sc-7691|sc-7692 from Santa Cruz Biotechnology.
Host Mouse
Isotype Mouse IgG1
Validated Species Bovine, Human, Mouse, Rat
Predicted Species Monkey

*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.

Application IHC-P, WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen Bovine kidney calbindin-D
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Monoclonal
Clone Number CB-955
Form Lyophilized
Contents Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
*carrier free antibody available upon request.
Concentration Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Ascites
Isotype Mouse IgG1

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name Calb1
Protein Name Calbindin
Molecular Weight 29994 MW
Protein Function Buffers cytosolic calcium. May stimulate a membrane Ca(2+)-ATPase and a 3',5'-cyclic nucleotide phosphodiesterase.
Sequence Similarities Belongs to the calbindin family.
Uniprot ID P07171
Alternative Names Calbindin;Calbindin D28;D-28K;Spot 35 protein;Vitamin D-dependent calcium-binding protein, avian-type;Calb1;
Research Areas |neuroscience|neurotransmission|calcium signaling|calcium binding proteins| neuroscience|cell type marker|neuron marker|soma marker|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Calbindin

The human calbindin D28k(CALB1) is a calcium-binding protein belonging to the troponin C superfamily. It was originally described as a 27-kD protein induced by vitamin D in the duodenum of the chick. Parmentier et al.(1991) mapped the CALB1 gene to 8q21.3-q22.1 by in situ hybridization.

Anti-Calbindin-D Antibody (Monoclonal, CB-955) Images

Click the images to enlarge.

Anti-Calbindin-D Antibody (Monoclonal, CB-955)
Anti-Calbindin-D antibody (monoclonal), MA1007, Western blotting
Lane 1: Rat Brain Tissue Lysate
Lane 2: Rat Brain Tissue Lysate
Lane 3: Rat Kidney Tissue Lysate
Lane 4: Rat Lung Tissue Lysate

Anti-Calbindin-D Antibody (Monoclonal, CB-955)
Anti-Calbindin-D antibody (monoclonal), MA1007, IHC(P)
IHC(P): Mouse Kidney Tissue
Anti-Calbindin-D Antibody (Monoclonal, CB-955)
Anti-Calbindin-D antibody (monoclonal), MA1007, IHC(P)
IHC(P): Rat Kidney Tissue
Anti-Calbindin-D Antibody (Monoclonal, CB-955)
Anti-Calbindin-D antibody (monoclonal), MA1007, IHC(P)
IHC(P): Human Intestinal Cancer Tissue
Anti-Calbindin-D Antibody (Monoclonal, CB-955)
Anti-Calbindin-D antibody (monoclonal), MA1007, IHC(P)
IHC(P): Human Mammary Cancer Tissue
Write a review for MA1007

FAQs

Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: Can you provide monoclonal antibodies carrier free?
A: We cannot.