Anti-Calbindin Rabbit Monoclonal Antibody

SKU M03047
Size 100ug/vial
Reactivity Human, Mouse, Rat
Clonality Monoclonal
Clone Number AAAA-3
Host Rabbit
Applications IF, WB

Overview

Product Name Anti-Calbindin Rabbit Monoclonal Antibody
SKU/Catalog Number M03047
Storage & Handling At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
Size 100ug/vial
Description Anti-Calbindin Rabbit Monoclonal Antibodytested for IF, IHC, ICC, WB in Human, Mouse, Rat
Cite This Product Anti-Calbindin Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03047)
Host Rabbit
Contents/Buffer Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form Liquid
Immunogen A synthesized peptide derived from human Calbindin
Reactivity Human, Mouse, Rat

Assay Details

Assay Dilutions Overview

Concentration: 0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200

Images And Assay Conditions

Western blot analysis of Calbindin expression in human fetal brain lysate (M03047).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CALB1 monoclonal antibody (Catalog # M03047) overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CALB1

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id P05937
Gene Name CALB1
Protein Name Calbindin
Alternative Names Calbindin;Calbindin D28;D-28K;Vitamin D-dependent calcium-binding protein, avian-type;CALB1;CAB27;
Molecular Weight 30025 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function Buffers cytosolic calcium. May stimulate a membrane Ca(2+)-ATPase and a 3',5'-cyclic nucleotide phosphodiesterase.
Research Areas Human, Mouse, Rat

*You can search these to find other products in these research areas.

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Monoclonal antibody for CALBINDIN/CALB1 detection. Host: Rabbit.Size: 100ug/vial. Tested applications: IF, IHC, ICC, WB. Reactive species: Human, Mouse, Rat CALBINDIN/CALB1 information: Molecular Weight: 30025 MW; .
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In stock
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M03047
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$299.00

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