Product Info Summary
SKU: | A07303 |
---|---|
Size: | 50μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IP, WB |
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Product info
Product Name
Anti-Calcineurin A PPP3CB Antibody
SKU/Catalog Number
A07303
Size
50μg/vial
Form
Liquid
Description
Boster Bio Anti-Calcineurin A PPP3CB Antibody catalog # A07303. Tested in IP, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for long-term storage. Avoid freeze/thaw cycles.
Cite This Product
Anti-Calcineurin A PPP3CB Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07303)
Host
Rabbit
Contents
Each vial contains 50% glycerol and 0.09% sodium azide.
Clonality
Polyclonal
Immunogen
Synthetic peptide corresponding to human calcineurin A.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A07303 is reactive to PPP3CB in Human, Mouse, Rat
Applications
A07303 is guaranteed for IP, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
59.024kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:1, 000, ECL, Human, Mouse, Rat
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of Calcineurin A expression in rat brain extract (lane 1) and mouse brain extract (lane 2). Calcineurin A at 61KD was detected using rabbit anti-Calcineurin A Antigen Affinity purified polyclonal antibody (Catalog # A07303) at 1: 1000. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1002).
Protein Target Info & Infographic
Gene/Protein Information For PPP3CB (Source: Uniprot.org, NCBI)
Gene Name
PPP3CB
Full Name
Serine/threonine-protein phosphatase 2B catalytic subunit beta isoform
Weight
59.024kDa
Superfamily
PPP phosphatase family
Alternative Names
Calmodulin-dependent calcineurin A subunit beta isoform; CALNA2protein phosphatase 3 (formerly 2B), catalytic subunit, beta isoform(calcineurin A beta); CAM-PRP catalytic subunit; CNA2catalytic subunit, beta isoform; EC 3.1.3.16; protein phosphatase 2B, catalytic subunit, beta isoform; protein phosphatase 3, catalytic subunit, beta isozyme; protein phosphatase from PCR fragment H32; serine/threonine-protein phosphatase 2B catalytic subunit beta isoform PPP3CB CALNA2, CALNB, CNA2, PP2Bbeta protein phosphatase 3 catalytic subunit beta serine/threonine-protein phosphatase 2B catalytic subunit beta isoform|CAM-PRP catalytic subunit|CNA beta|calcineurin A beta|calcineurin A2|protein phosphatase 3, catalytic subunit, beta isozyme|protein phosphatase from PCR fragment H32
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PPP3CB, check out the PPP3CB Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PPP3CB: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Calcineurin A PPP3CB Antibody (A07303)
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No publications found for A07303
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-Calcineurin A PPP3CB Antibody
Question
We are interested in to test anti-Calcineurin A antibody A07303 on rat testis for research purposes, then I may be interested in using anti-Calcineurin A antibody A07303 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-09-11
Answer
The products we sell, including anti-Calcineurin A antibody A07303, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-09-11
Question
Will anti-Calcineurin A antibody A07303 work on dog IP with testis?
Verified Customer
Verified customer
Asked: 2019-06-14
Answer
Our lab technicians have not validated anti-Calcineurin A antibody A07303 on dog. You can run a BLAST between dog and the immunogen sequence of anti-Calcineurin A antibody A07303 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated dog samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in dog testis in IP, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-06-14
Question
Will anti-Calcineurin A antibody A07303 work for WB with testis?
Verified Customer
Verified customer
Asked: 2019-03-26
Answer
According to the expression profile of testis, PPP3CB is highly expressed in testis. So, it is likely that anti-Calcineurin A antibody A07303 will work for WB with testis.
Boster Scientific Support
Answered: 2019-03-26
Question
We are currently using anti-Calcineurin A antibody A07303 for rat tissue, and we are well pleased with the IP results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2019-03-19
Answer
The anti-Calcineurin A antibody (A07303) has not been validated for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-03-19
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for testis using anti-Calcineurin A antibody A07303. Let me know if you need anything else.
W. Krishna
Verified customer
Asked: 2018-11-26
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-11-26
Question
Do you have a BSA free version of anti-Calcineurin A antibody A07303 available?
C. Edwards
Verified customer
Asked: 2015-05-01
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-Calcineurin A antibody A07303 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2015-05-01
Question
My question regarding product A07303, anti-Calcineurin A antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
V. Li
Verified customer
Asked: 2014-06-03
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A07303 anti-Calcineurin A antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2014-06-03