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SKU:MA1009
Clonality:Monoclonal
Clone Number:hHCD
Application:IHC-P, WB
Price: $200.00
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Overview

Product Name Anti-Caldesmon (Smooth) Antibody (Monoclonal, hHCD)
SKU/Catalog Number MA1009
Description Mouse IgG monoclonal antibody for Caldesmon (smooth), caldesmon 1 (CALD1) detection. Tested with WB, IHC-P in Human;mouse;rabbit. No cross reactivity with other proteins.
Cite This Product Anti-Caldesmon (Smooth) Antibody (Monoclonal, hHCD) hHCD (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1009)
Replacement Item This antibody may replace the following items: sc-12930-R|sc-12931|sc-12931-R|sc-135965|sc-15374|sc-25339|sc-271222|sc-48427|sc-58700|sc-58703|sc-7574|sc-7575|sc-7575-R from Santa Cruz Biotechnology.
Host Mouse
Isotype Mouse IgG1
Validated Species Human, Mouse, Rabbit
Application IHC-P, WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen Human uterus smooth muscle extract.
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Monoclonal
Clone Number hHCD
Form Liquid
Contents Each vial contains 50% glycerol, 1.2% Sodium acetate, 1% BSA, 0.02% NaN3.
*carrier free antibody available upon request.
Storage At -20˚C for one year, at 4˚C for one month. Avoid repeated freezing and thawing.
Purification Ascites
Isotype Mouse IgG1

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name Cald1
Protein Name Caldesmon
Molecular Weight 60584 MW
Protein Function Actin- and myosin-binding protein implicated in the regulation of actomyosin interactions in smooth muscle and nonmuscle cells (could act as a bridge between myosin and actin filaments). Stimulates actin binding of tropomyosin which increases the stabilization of actin filament structure. In muscle tissues, inhibits the actomyosin ATPase by binding to F-actin. This inhibition is attenuated by calcium-calmodulin and is potentiated by tropomyosin. Interacts with actin, myosin, two molecules of tropomyosin and with calmodulin. Also play an essential role during cellular mitosis and receptor capping. Involved in Schwann cell migration during peripheral nerve regeneration. .
Tissue Specificity High-molecular-weight caldesmon (h-caldesmon) is predominantly expressed in smooth muscles, whereas low- molecular-weight caldesmon (l-caldesmon) is widely distributed in non-muscle tissues and cells. Not expressed in skeletal muscle or heart.
Subcellular Localization Cytoplasm, cytoskeleton . Cytoplasm, myofibril . On thin filaments in smooth muscle and on stress fibers in fibroblasts (nonmuscle). .
Uniprot ID Q62736
Alternative Names Non-muscle caldesmon;CDM;L-caldesmon;Cald1;
Research Areas |signal transduction|cytoskeleton / ecm|cytoskeleton|microfilaments|actin etc|actin binding proteins| cardiovascular|heart|contractility|contractile proteins|actins|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Caldesmon

Caldesmon is a potential actomyosin regulatory protein found in smooth muscle and nonmuscle cells. The predicted smooth-muscle polypeptide is 793 amino acids long. The high molecular weight caldesmon(h-CaD) is predominantly expressed in smooth muscles, whereas the low molecular weight caldesmon(l-CaD) is widely distributed in nonmuscle tissues and cells. Hayashi et al.(1992) demonstrated that the human CDM gene is composed of 14 exons.

Anti-Caldesmon (Smooth) Antibody (Monoclonal, hHCD) Images

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Anti-Caldesmon (Smooth) Antibody (Monoclonal, hHCD)
Anti-Caldesmon(smooth) antibody, MA1009, IHC(P)
IHC(P): Human Intestinal Cancer Tissue
Anti-Caldesmon (Smooth) Antibody (Monoclonal, hHCD)
Anti-Caldesmon(smooth) antibody, MA1009, IHC(P)
IHC(P): Human Mammary Cancer Tissue
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FAQs

Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: Can you provide monoclonal antibodies carrier free?
A: We cannot.
Q: What are some alternative names that could be used to describe this product?
A: One other very common name is caldesmon antibody