Data & Images
|Product Name||Anti-Caldesmon (Smooth) Antibody (Monoclonal, hHCD)|
|Description||Mouse IgG monoclonal antibody for Caldesmon (smooth), caldesmon 1 (CALD1) detection. Tested with WB, IHC-P in Human;mouse;rabbit. No cross reactivity with other proteins.|
|Cite This Product||Anti-Caldesmon (Smooth) Antibody (Monoclonal, hHCD) hHCD (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1009)|
|Replacement Item||This antibody may replace the following items: sc-12930-R|sc-12931|sc-12931-R|sc-135965|sc-15374|sc-25339|sc-271222|sc-48427|sc-58700|sc-58703|sc-7574|sc-7575|sc-7575-R from Santa Cruz Biotechnology.|
|Validated Species||Human, Mouse, Rabbit|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2016!
|Immunogen||Human uterus smooth muscle extract.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 50% glycerol, 1.2% Sodium acetate, 1% BSA, 0.02% NaN3.
*carrier free antibody available upon request.
|Storage||At -20˚C for one year, at 4˚C for one month. Avoid repeated freezing and thawing.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Gene Full Name||Non-muscle caldesmon|
|Protein Function||Actin- and myosin-binding protein implicated in theregulation of actomyosin interactions in smooth muscle andnonmuscle cells (could act as a bridge between myosin and actinfilaments). Stimulates actin binding of tropomyosin whichincreases the stabilization of actin filament structure. In muscletissues, inhibits the actomyosin ATPase by binding to F-actin.This inhibition is attenuated by calcium-calmodulin and ispotentiated by tropomyosin. Interacts with actin, myosin, twomolecules of tropomyosin and with calmodulin. Also play anessential role during cellular mitosis and receptor capping.Involved in Schwann cell migration during peripheral nerveregeneration..|
|Tissue Specificity||High-molecular-weight caldesmon (h-caldesmon)is predominantly expressed in smooth muscles, whereas low-molecular-weight caldesmon (l-caldesmon) is widely distributed innon-muscle tissues and cells. Not expressed in skeletal muscle orheart.|
|Subcellular Localization||Cytoplasm, cytoskeleton.Cytoplasm, myofibril. On thin filaments insmooth muscle and on stress fibers in fibroblasts (nonmuscle)..|
|Uniprot ID||Cald1: Q62736
|Alternative Names||Non-muscle caldesmon; CDM; L-caldesmon; Cald1;|
|Research Areas||signal transduction|cytoskeleton / ecm|cytoskeleton|microfilaments|actin etc|actin binding proteins|cardiovascular|heart|contractility|contractile proteins|actins||
Background for Caldesmon
At Boster we strive to provide the best Anti-Caldesmon (Smooth) Antibody (Monoclonal, hHCD) by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Application||Concentration*||Tested Species||Predicted Species||Antigen Retrieval|
|Immunohistochemistry(Paraffin-embedded Section)||0.5-1μg/ml||Human, mouse, rabbit||-||By Heat|
|Western blot||0.25-0.5μg/ml||Human, mouse, rabbit||-|
Other applications have not been tested.
Optimal dilutions should be determined by end users.
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-Caldesmon (Smooth) Antibody (Monoclonal, hHCD) Images
Click the images to enlarge.
IHC(P): Human Intestinal Cancer Tissue
IHC(P): Human Mammary Cancer Tissue