SKU MA1010
Size 100μg/vial
Reactivity Chicken, Rat
Clonality Monoclonal
Clone Number 6D4
Host Mouse
Ig Isotype Mouse IgG1
Applications WB

Overview

Product Name Anti-Calmodulin Antibody (Monoclonal, 6D4)
SKU/Catalog Number MA1010
Storage & Handling At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.
Size 100μg/vial
Description Mouse IgG monoclonal antibody for Calmodulin, calmodulin 1; calmodulin 2; calmodulin 3 (CALM1; CALM2; CALM3) detection. Tested with WB in chicken;rat. No cross reactivity with other proteins.
Cite This Product Anti-Calmodulin Antibody (Monoclonal, 6D4) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1010)
Host Mouse
Contents/Buffer Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
Immunogen Preparation of purified calmodulin from Dictyostelium discoideum conjugated to KLH.
Reactivity Chicken, Rat

Assay Details

Assay Dilutions Overview

Concentration: Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Western blot, 0.5μg/ml, Chicken, rat

Boster's Secondary Antibodies And IHC, WB Kits

The following reagents are used to generate the images below.

Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot.

Images And Assay Conditions

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Boster Kit Box

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id P62161
Gene Name CALM1; CALM2; CALM3
Protein Name Calmodulin
Alternative Names Calmodulin;CaM;Calm1;Calm, Cam, Cam1;Calm2;Cam2, Camb;Calm3;Cam3, Camc;
Subcellular Localization Cytoplasm, cytoskeleton, spindle. Cytoplasm, cytoskeleton, spindle pole. Distributed throughout the cell during interphase, but during mitosis becomes dramatically localized to the spindle poles and the spindle microtubules. .
Molecular Weight 16838 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function Calmodulin mediates the control of a large number of enzymes, ion channels, aquaporins and other proteins by Ca(2+). Among the enzymes to be stimulated by the calmodulin-Ca(2+) complex are a number of protein kinases and phosphatases. Together with CCP110 and centrin, is involved in a genetic pathway that regulates the centrosome cycle and progression through cytokinesis (By similarity). .
Research Areas Apoptosis, Atherosclerosis, Calcium Signaling, Calmodulin Pathway, Cancer, Cancer Metabolism, Carbohydrate Metabolism, Cardiovascular, Heart Disease, Metabolic Signaling Pathway, Metabolic Signaling Pathways, Metabolism, Metabolism Of Carbohydrates, Pathways And Processes, Signal Transduction, Signaling Pathway

*You can search these to find other products in these research areas.
Background Calmodulin is the archetype of the family of calcium-modulated proteins of which nearly 20 members have been found. Calmodulin contains 149 amino acids and has 4 calcium-binding domains. Its functions include roles in growth and the cell cycle as well as in signal transduction and the synthesis and release of neurotransmitters. Three calmodulin genes(CALM1, CALM2, and CALM3) map to chromosomes 14q24-q31, 2p21.1-p21.3, and 19q13.2-q13.3, respectively.

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Order Product (MA1010)

Promotion:

Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
Option Price
30ug sample size $99
100ug $240
100ug+Free HRP Secondary BA1050 $240
100ug+Free Biotin Secondary BA1001 $240

USD $240

Ships in 7-10 business days.

Troubleshooting

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Customer Q&As

Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: Can you provide monoclonal antibodies carrier free?
A: We cannot.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to calmodulin antibody, cam antibody