Data & Images
|Product Name||Anti-Calmodulin Antibody (Monoclonal, 6D4)|
|Description||Mouse IgG monoclonal antibody for Calmodulin, calmodulin 1; calmodulin 2; calmodulin 3 (CALM1; CALM2; CALM3) detection. Tested with WB in chicken;rat. No cross reactivity with other proteins.|
|Cite This Product||Anti-Calmodulin Antibody (Monoclonal, 6D4) 6D4 (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1010)|
|Replacement Item||This antibody may replace the following items: sc-137079|sc-17018-R|sc-17019|sc-17019-R|sc-17022-R|sc-17023-R|sc-1989|sc-23760-R|sc-23761-R|sc-34082|sc-5537 from Santa Cruz Biotechnology.|
|Validated Species||Chicken, Rat|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2016!
|Immunogen||Preparation of purified calmodulin from Dictyostelium discoideum conjugated to KLH.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
*carrier free antibody available upon request.
|Concentration||Add 1ml of PBS buffer will yield a concentration of 100ug/ml.|
|Storage||At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Gene Full Name||Calmodulin|
|Protein Function||Calmodulin mediates the control of a large number ofenzymes, ion channels, aquaporins and other proteins by Ca(2+).Among the enzymes to be stimulated by the calmodulin-Ca(2+)complex are a number of protein kinases and phosphatases. Togetherwith CCP110 and centrin, is involved in a genetic pathway thatregulates the centrosome cycle and progression through cytokinesis(By similarity)..|
|Sequence Similarities||Belongs to the calmodulin family.|
|Subcellular Localization||Cytoplasm, cytoskeleton, spindle. Cytoplasm,cytoskeleton, spindle pole. Distributed throughout the cellduring interphase, but during mitosis becomes dramaticallylocalized to the spindle poles and the spindle microtubules..|
|Uniprot ID||Calm1: P62161
|Alternative Names||Calmodulin; CaM; Calm1; Calm, Cam, Cam1; Calm2; Cam2, Camb; Calm3; Cam3, Camc;|
|Research Areas||signal transduction|signaling pathway|calcium signaling|calmodulin pathway|cardiovascular|atherosclerosis|apoptosis|heart|cardiac metabolism|cancer|cancer metabolism|metabolic signaling pathway|metabolism of carbohydrates|pathways and processes|metabolic signaling pathways|carbohydrate metabolism|types of disease|heart disease||
Background for Calmodulin
At Boster we strive to provide the best Anti-Calmodulin Antibody (Monoclonal, 6D4) by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Application||Concentration*||Tested Species||Predicted Species||Antigen Retrieval|
|Western blot||0.5μg/ml||Chicken, rat||-|
Other applications have not been tested.
Optimal dilutions should be determined by end users.
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.