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SKU:MA1010
Pack Size:100μg/vial
Sample Size:30ug for $99, contact us for details
Clonality:Monoclonal
Clone Number:6D4
Application:WB
Price: $200.00
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Overview

Product Name Anti-Calmodulin Antibody (Monoclonal, 6D4)
SKU/Catalog Number MA1010
Description Mouse IgG monoclonal antibody for Calmodulin, calmodulin 1; calmodulin 2; calmodulin 3 (CALM1; CALM2; CALM3) detection. Tested with WB in chicken;rat. No cross reactivity with other proteins.
Cite This Product Anti-Calmodulin Antibody (Monoclonal, 6D4) 6D4 (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1010)
Replacement Item This antibody may replace the following items: sc-137079|sc-17018-R|sc-17019|sc-17019-R|sc-17022-R|sc-17023-R|sc-1989|sc-23760-R|sc-23761-R|sc-34082|sc-5537 from Santa Cruz Biotechnology.
Host Mouse
Isotype Mouse IgG1
Validated Species Chicken, Rat
Predicted Species Chicken

*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.

Application WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen Preparation of purified calmodulin from Dictyostelium discoideum conjugated to KLH.
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Monoclonal
Clone Number 6D4
Form Lyophilized
Contents Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
*carrier free antibody available upon request.
Concentration Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Ascites
Isotype Mouse IgG1

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name Calm1
Protein Name Calmodulin
Molecular Weight 16838 MW
Protein Function Calmodulin mediates the control of a large number of enzymes, ion channels, aquaporins and other proteins by Ca(2+). Among the enzymes to be stimulated by the calmodulin-Ca(2+) complex are a number of protein kinases and phosphatases. Together with CCP110 and centrin, is involved in a genetic pathway that regulates the centrosome cycle and progression through cytokinesis (By similarity). .
Sequence Similarities Belongs to the calmodulin family.
Subcellular Localization Cytoplasm, cytoskeleton, spindle. Cytoplasm, cytoskeleton, spindle pole. Distributed throughout the cell during interphase, but during mitosis becomes dramatically localized to the spindle poles and the spindle microtubules. .
Uniprot ID P62161
Alternative Names Calmodulin;CaM;Calm1;Calm, Cam, Cam1;Calm2;Cam2, Camb;Calm3;Cam3, Camc;
Research Areas signal transduction|signaling pathway|calcium signaling|calmodulin pathway|cardiovascular|atherosclerosis|apoptosis|heart|cardiac metabolism|cancer|cancer metabolism|metabolic signaling pathway|metabolism of carbohydrates|pathways and processes|metabolic signaling pathways|carbohydrate metabolism|types of disease|heart disease|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Calmodulin

Calmodulin is the archetype of the family of calcium-modulated proteins of which nearly 20 members have been found. Calmodulin contains 149 amino acids and has 4 calcium-binding domains. Its functions include roles in growth and the cell cycle as well as in signal transduction and the synthesis and release of neurotransmitters. Three calmodulin genes(CALM1, CALM2, and CALM3) map to chromosomes 14q24-q31, 2p21.1-p21.3, and 19q13.2-q13.3, respectively.
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FAQs

Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: Can you provide monoclonal antibodies carrier free?
A: We cannot.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to calmodulin antibody, cam antibody