Anti-Calponin Antibody (Monoclonal, CP-93)
Mouse IgG monoclonal antibody for Calponin, calponin 2 (CNN2) detection. Tested with WB, IHC-P, ICC in Human;chicken;turkey. No cross reactivity with other proteins.
|Reactivity||Chicken, Human, Turkey|
|Ig Isotype||Mouse IgG1|
|Applications||IHC, ICC, WB|
|Product Name||Anti-Calponin Antibody (Monoclonal, CP-93)
See all Cnn2 primary antibodies, ELISA kits and proteins
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Monoclonal clone# G2 antibody for CNN2 detection. Host: Mouse.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Chicken. CNN2 information: Molecular Weight: 33156 MW; Tissue Specificity: Smooth muscle, and tissues containing significant amounts of smooth muscle.|
|Cite This Product||Anti-Calponin Antibody (Monoclonal, CP-93) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1011)|
|Specificity||Anti-Calponin Antibody (Monoclonal, CP-93) (MA1011) reacts with Chicken, Human, Turkey Cnn2, in native form and recombinant. Superfamily members of Cnn2 are not reactive to MA1011.|
|Contents/Buffer||Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.|
|Reconstitution||Add 1ml of PBS buffer will yield a concentration of 100ug/ml.|
|Immunogen||Turkey gizzard calponin|
|Reactivity||Chicken, Human, Turkey|
Our Boster Quality Guarantee for Anti-Calponin Antibody (Monoclonal, CP-93) covers its use in the following applications.
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
Assay Dilutions Overview
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, chicken, turkey, By Heat
Immunocytochemistry , 1μg/ml, Human, chicken, turkey, -
Western blot, 0.25-0.5μg/ml, Human, chicken, turkey
Boster's Compatible Products
The following reagents are used to generate the images below for Anti-Calponin Antibody (Monoclonal, CP-93) (MA1011).Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and ICC.
Images And Assay Conditions
Boster Kit Box
Protein Target Info (Source: Uniprot.org)
|Tissue Specificity||Smooth muscle, and tissues containing significant amounts of smooth muscle.|
|Alternative Names||Calponin-2;Calponin H2, smooth muscle;Neutral calponin;Cnn2;|
|Molecular Weight||33156 MW|
*if product is indicated to react with multiple species, protein info is based on the human gene.
|Protein Function||Thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin, troponin C and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity.|
|Research Areas||Chicken, Human, Turkey
*You can search these to find other products in these research areas.
|Background||Calponin is a basic 34-kD protein first isolated from chicken gizzard and bovine aorta. It is specifically expressed in smooth muscle and binds calmodulin, actin, and tropomyosin. It is able to inhibit the ATPase activity of myosin and is though to play a role in smooth muscle contraction. Calponin is a smooth muscle specific, actin-, tropomyosin- and calmodulin-binding protein though to be involved in some way in the regulation or modulation of contraction.|
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Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WBA: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugationA: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact [email protected]
Q: Can you provide monoclonal antibodies carrier free?A: For most of our monoclonal antibodies, we are unable to provide them carrier free. If you would like to check if a specific antibody can be provided carrier free, please send an email to [email protected]