|Application:||IHC-P, ICC, WB|
Data & Images
|Product Name||Anti-Calponin Antibody (Monoclonal, CP-93)|
|Description||Mouse IgG monoclonal antibody for Calponin, calponin 2 (CNN2) detection. Tested with WB, IHC-P, ICC in Human;chicken;turkey. No cross reactivity with other proteins.|
|Cite This Product||Anti-Calponin Antibody (Monoclonal, CP-93) CP-93 (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1011)|
|Replacement Item||This antibody may replace the following items: sc-133188|sc-136986|sc-136987|sc-16607|sc-16608|sc-28545 from Santa Cruz Biotechnology.|
|Validated Species||Chicken, Human, Turkey|
|Application||IHC-P, ICC, WB
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and ICC.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2016!
|Immunogen||Turkey gizzard calponin|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
*carrier free antibody available upon request.
|Concentration||Add 1ml of PBS buffer will yield a concentration of 100ug/ml.|
|Storage||At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Gene Full Name||Calponin-2|
|Protein Function||Thin filament-associated protein that is implicated inthe regulation and modulation of smooth muscle contraction. It iscapable of binding to actin, calmodulin, troponin C andtropomyosin. The interaction of calponin with actin inhibits theactomyosin Mg-ATPase activity.|
|Tissue Specificity||Smooth muscle, and tissues containingsignificant amounts of smooth muscle.|
|Sequence Similarities||Belongs to the calponin family.|
|Uniprot ID||Cnn2: Q08093
|Alternative Names||Calponin-2; Calponin H2, smooth muscle; Neutral calponin; Cnn2;|
|Research Areas||signal transduction|cytoskeleton / ecm|cytoskeleton|microfilaments|actin etc|actin binding proteins|cardiovascular|heart|contractility|contractile proteins|actins||
Background for Calponin-2
At Boster we strive to provide the best Anti-Calponin Antibody (Monoclonal, CP-93) by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Application||Concentration*||Tested Species||Predicted Species||Antigen Retrieval|
|Immunohistochemistry(Paraffin-embedded Section)||0.5-1μg/ml||Human, chicken, turkey||-||By Heat|
|Immunocytochemistry||1μg/ml||Human, chicken, turkey||-|
|Western blot||0.25-0.5μg/ml||Human, chicken, turkey||-|
Other applications have not been tested.
Optimal dilutions should be determined by end users.
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.