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SKU:MA1011
Clonality:Monoclonal
Clone Number:CP-93
Application:IHC-P, ICC, WB
Price: $200.00
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Overview

Product Name Anti-Calponin Antibody (Monoclonal, CP-93)
Description Mouse IgG monoclonal antibody for Calponin, calponin 2 (CNN2) detection. Tested with WB, IHC-P, ICC in Human;chicken;turkey. No cross reactivity with other proteins.
Cite This Product Anti-Calponin Antibody (Monoclonal, CP-93) CP-93 (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1011)
Replacement Item This antibody may replace the following items: sc-133188|sc-136986|sc-136987|sc-16607|sc-16608|sc-28545 from Santa Cruz Biotechnology.
Host Mouse
Isotype Mouse IgG1
Validated Species Chicken, Human, Turkey
Application IHC-P, ICC, WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and ICC.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2016!
Immunogen Turkey gizzard calponin
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Monoclonal
Clone Number CP-93
Form Lyophilized
Contents Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
*carrier free antibody available upon request.
Concentration Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Ascites
Isotype Mouse IgG1

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name Cnn2
Protein Name Calponin-2
Gene Full Name Calponin-2
Protein Function Thin filament-associated protein that is implicated inthe regulation and modulation of smooth muscle contraction. It iscapable of binding to actin, calmodulin, troponin C andtropomyosin. The interaction of calponin with actin inhibits theactomyosin Mg-ATPase activity.
Tissue Specificity Smooth muscle, and tissues containingsignificant amounts of smooth muscle.
Sequence Similarities Belongs to the calponin family.
Uniprot ID Cnn2: Q08093
Alternative Names Calponin-2; Calponin H2, smooth muscle; Neutral calponin; Cnn2;
Research Areas signal transduction|cytoskeleton / ecm|cytoskeleton|microfilaments|actin etc|actin binding proteins|cardiovascular|heart|contractility|contractile proteins|actins|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Calponin-2

Calponin is a basic 34-kD protein first isolated from chicken gizzard and bovine aorta. It is specifically expressed in smooth muscle and binds calmodulin, actin, and tropomyosin. It is able to inhibit the ATPase activity of myosin and is though to play a role in smooth muscle contraction. Calponin is a smooth muscle specific, actin-, tropomyosin- and calmodulin-binding protein though to be involved in some way in the regulation or modulation of contraction.
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Publications

Liu C, Chen L, Zeng J, Cui J, Ning Jn, Wang Gs, Belguise K, Wang X, Qian Gs, Lu Kz, Yi B. Exp Cell Res. 2015 Aug 1;336(1):109-18. Doi: 10.1016/J.Yexcr.2015.05.025. Epub 2015 Jun 10. Bone Morphogenic Protein-2 Regulates The Myogenic Differentiation...

FAQs

Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: Can you provide monoclonal antibodies carrier free?
A: We cannot.