Anti-CAND1 Antibody

TIP120A antibody

Boster Bio Anti-CAND1 Antibody (Catalog # A03609). Tested in IP, WB applications. This antibody reacts with Human.

Product Info Summary

SKU: A03609
Size: 100uL
Reactive Species: Human
Host: Rabbit
Application: IP, WB

Customers Who Bought This Also Bought

Product Name

Anti-CAND1 Antibody

View all TIP120A Antibodies

SKU/Catalog Number

A03609

Size

100uL

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-CAND1 Antibody (Catalog # A03609). Tested in IP, WB applications. This antibody reacts with Human.

Storage & Handling

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Cite This Product

Anti-CAND1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03609)

Host

Rabbit

Contents

0.01% (w/v) Sodium Azide

Clonality

Polyclonal

Clone Number

1H1D2

Isotype

Antiserum

Immunogen

This antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 11-24 of Human CAND1/TIP120A (N-terminal) coupled to KLH.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A03609 is reactive to CAND1 in Human

Applications

A03609 is guaranteed for IP, WB Boster Guarantee

Observed Molecular Weight

39 kDa

Calculated molecular weight

136.376kDa

Background of TIP120A

CAND1 is also known as TIP120A, and TATA-binding protein-interacting protein 120A. The SCF complex consists of the invariable components Skp1, Cul1, and Rbx1 as well as a variable F-box protein, and functions as an E3 ubiquitin ligase. E3 ubiquitin ligases regulate various physiological processes. CAND1 binds to Cul1 and potentially regulates the SCF complex. CAND1 physically associates with Cul1 in the nucleus and this interaction is mediated by a central region of Cul1 distinct from its binding sites for Skp1 and Rbx1. CAND1 selectively binds to unneddylated CUL1 and is dissociated by CUL1 neddylation. CAND1 forms a ternary complex with CUL1 and ROC1.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.

Submit A Review

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:2,000 - 1:10,000
IHC: User optimized
IP: User optimized
WB: 1:500 - 1:1,000

Validation Images & Assay Conditions

Gene/Protein Information For CAND1 (Source: Uniprot.org, NCBI)

Gene Name

CAND1

Full Name

Cullin-associated NEDD8-dissociated protein 1

Weight

136.376kDa

Superfamily

CAND family

Alternative Names

cullin-associated and neddylation-dissociated 1; Cullin-associated and neddylation-dissociated protein 1; cullin-associated NEDD8-dissociated protein 1; DKFZp434M1414; FLJ90441; KIAA0829FLJ10114; p120 CAND1; TBP interacting protein; TBP-interacting protein 120A; TBP-interacting protein of 120 kDa A; TIP120AFLJ38691; TIP120FLJ10929 CAND1 TIP120, TIP120A cullin associated and neddylation dissociated 1 cullin-associated NEDD8-dissociated protein 1|TBP-interacting protein of 120 kDa A|p120 CAND1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on CAND1, check out the CAND1 Infographic

CAND1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for CAND1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

No publications found for A03609

*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.

Have you used Anti-CAND1 Antibody?

Submit a review and receive an Amazon gift card.

  • $25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
  • $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit A Review

Be the first to review Anti-CAND1 Antibody

*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.

Have a question?

Find answers in Q&As, reviews.

Can't find your answer?

Submit your question

6 Customer Q&As for Anti-CAND1 Antibody

Question

Is this A03609 anti-CAND1 antibody reactive to the isotypes of CAND1?

Verified Customer

Verified customer

Asked: 2019-12-11

Answer

The immunogen of A03609 anti-CAND1 antibody is This antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 11-24 of Human CAND1/TIP120A (N-terminal) coupled to KLH. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-12-11

Question

Will A03609 anti-CAND1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-10-11

Answer

As indicated on the product datasheet, A03609 anti-CAND1 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-10-11

Question

I have a question about product A03609, anti-CAND1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

L. Thomas

Verified customer

Asked: 2017-06-21

Answer

We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A03609 anti-CAND1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2017-06-21

Question

Would anti-CAND1 antibody A03609 work for WB with embryonic kidney?

C. Baker

Verified customer

Asked: 2015-03-19

Answer

According to the expression profile of embryonic kidney, CAND1 is highly expressed in embryonic kidney. So, it is likely that anti-CAND1 antibody A03609 will work for WB with embryonic kidney.

Boster Scientific Support

Answered: 2015-03-19

Question

I was wanting to use your anti-CAND1 antibody for WB for human embryonic kidney on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human embryonic kidney identification?

S. Parker

Verified customer

Asked: 2014-09-26

Answer

You can see on the product datasheet, A03609 anti-CAND1 antibody has been validated for IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human embryonic kidney in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2014-09-26

Question

We are currently using anti-CAND1 antibody A03609 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on dog tissues as well?

N. Anderson

Verified customer

Asked: 2014-07-15

Answer

The anti-CAND1 antibody (A03609) has not been tested for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2014-07-15

Order DetailsPrice
A03609

100uL

$599

Free Secondary Antibody

Choose an option to show lead time.
Get A Quote
Eddy test
In stock
Order Product
A03609
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$599.00

Troubleshooting

troubleshooting-box-image

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

Guaranteed product quality

We promise all of our products perform as described in datasheets.