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SKU:PA1513
Pack Size:100μg/vial
Sample Size:30ug for $99, contact us for details
Clonality:Polyclonal
Application:IHC-P, WB
Price: $200.00
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Overview

Product Name Anti-Caspase-10 Antibody
SKU/Catalog Number PA1513
Description Rabbit IgG polyclonal antibody for Caspase-10(CASP10) detection. Tested with WB, IHC-P in Human.
Cite This Product Anti-Caspase-10 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1513)
Replacement Item This antibody may replace the following items: sc-134299|sc-22184|sc-393983|sc-6184|sc-6185|sc-6186|sc-7955 from Santa Cruz Biotechnology.
Host Rabbit
Isotype N/A
Validated Species Human
Application IHC-P, WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen A synthetic peptide corresponding to a sequence at the N-terminus of human CASP10(220-236aa VKTFLEALPRAAVYRMN).
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Polyclonal
Form Lyophilized
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
Concentration Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Immunogen affinity purified.
Isotype N/A

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name CASP10
Protein Name Caspase-10(CASP-10)
Molecular Weight 58951 MW
Protein Function Involved in the activation cascade of caspases responsible for apoptosis execution. Recruited to both Fas- and TNFR-1 receptors in a FADD dependent manner. May participate in the granzyme B apoptotic pathways. Cleaves and activates caspase- 3, -4, -6, -7, -8, and -9. Hydrolyzes the small- molecule substrates, Tyr-Val-Ala-Asp-

-AMC and Asp-Glu-Val-Asp-

-AMC. .
Tissue Specificity Detectable in most tissues. Lowest expression is seen in brain, kidney, prostate, testis and colon.
Sequence Similarities Belongs to the peptidase C14A family.
Uniprot ID Q92851
Alternative Names Caspase-10;CASP-10;3.4.22.63;Apoptotic protease Mch-4;FAS-associated death domain protein interleukin-1B-converting enzyme 2;FLICE2;ICE-like apoptotic protease 4;Caspase-10 subunit p23/17;Caspase-10 subunit p12;CASP10;MCH4;
Research Areas |cell biology|apoptosis|intracellular|caspases etc|caspases| cancer|invasion/microenvironment| cell biology|proteolysis / ubiquitin|proteolytic enzymes|other proteases| metabolism|pathways and processes|metabolism processes|cell death|apoptotic markers|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Caspase-10(CASP-10)

Caspase-10 is an enzyme that, in humans, is encoded by the CASP10 gene. The Caspase 10 gene contains 11 exons and spans about 48 kb. This gene is mapped to 2q33.1. It is transcribed in the centromere-to-telomere direction.This gene encodes a protein that is a member of the cysteine-aspartic acid protease(caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes that undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 3 and 7, and the protein itself is processed by caspase 8. Mutations in this gene are associated with apoptosis defects seen in type II autoimmune lymphoproliferative syndrome. Three alternatively spliced transcript variants encoding different isoforms have been described for this gene.

Anti-Caspase-10 Antibody Images

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Anti-Caspase-10 Antibody
Anti-Caspase-10 antibody, PA1513, Western blotting
Lane 1: COLO320 Cell Lysate
Lane 2: HELA Cell Lysate
Lane 3: SW620 Cell Lysate
Lane 4: RAJI Cell Lysate
Anti-Caspase-10 Antibody
Anti-Caspase-10 antibody, PA1513, IHC(P)
IHC(P): Human Intestinal Cancer Tissue
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Publications

Chen K, Shu G, Peng X, Fang J, Cui H, Chen J, Wang F, Chen Z, Zuo Z, Deng J, Geng Y, Lai W. Food Chem Toxicol. 2013 Sep;59:446-54. Doi: 10.1016/J.Fct.2013.06.032. Epub 2013 Jun 28. Protective Role Of Sodium Selenite On Histopathological Lesions, D...

FAQs

Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: I got a high background from the bands on my western blot gel. Here are the parameters that I used: - Protein loading 70 µg - SDS gel 10% - Electrophoresis 60V 60 min & 120V 95 min - Blocking 5% milk RT 30 min - Primary antibody 1:2000 dilution & 4°C overnight incubation - Secondary antibody α-Rabbit –HRP RT incubation for 1 hour. Please advise how we can lower the background.
A: Try to repeat your western blot with the following changes: - Protein loading 50 µg - Blocking 5% non-fat dry milk & incubate at 4°C for 2 hours with slow shaking - Primary antibody 1:4000 dilution. Then the background of your gel should be reduced.
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
A: You can find the immunogen sequence under "Immunogen" and clonality in the product name.
Q: What is the expected band size? Why is it different than the observed band size?
A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands

3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.

4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
A: Check our protocols under the tech support tab.
Q: What are some alternative names that could be used to describe this product?
A: One other very common name is caspase 10 antibody