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SKU:PA1302-1
Clonality:Polyclonal
Application:IHC-P, ICC, WB
Price: $200.00
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Overview

Product Name Anti-Caspase-3 (P10) Antibody
Description Rabbit IgG polyclonal antibody for Caspase-3(CASP3) detection. Tested with WB, IHC-P, ICC in Human.
Cite This Product Anti-Caspase-3 (P10) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1302-1)
Replacement Item This antibody may replace the following items: sc-1224|sc-1225|sc-1226|sc-136219|sc-166589|sc-22139|sc-22140|sc-22171-R|sc-271028|sc-271759|sc-373730|sc-56046|sc-56050|sc-56051|sc-56052|sc-56053|sc-6138|sc-65496|sc-65497|sc-70497|sc-70498|sc-7148|sc-7272|sc-98785 from Santa Cruz Biotechnology.
Host Rabbit
Isotype N/A
Validated Species Human
Predicted Species Hamster

*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.

Application IHC-P, ICC, WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P) and ICC.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen A synthetic peptide corresponding to a sequence at the C-terminus of human Caspase-3(P10)(220-236aa CAMLKQYADKLEFMHIL).
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Polyclonal
Form Lyophilized
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
Concentration Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Immunogen affinity purified.
Isotype N/A

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name CASP3
Protein Name Caspase-3(CASP-3)
Molecular Weight 31608 MW
Protein Function Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-

-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop- helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage. .
Tissue Specificity Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system.
Sequence Similarities Belongs to the peptidase C14A family.
Subcellular Localization Cytoplasm.
Uniprot ID P42574
Alternative Names Caspase-3;CASP-3;3.4.22.56;Apopain;Cysteine protease CPP32;CPP-32;Protein Yama;SREBP cleavage activity 1;SCA-1;Caspase-3 subunit p17;Caspase-3 subunit p12;CASP3;CPP32;
Research Areas |neuroscience|neurology process|neurodegenerative disease|alzheimer's disease| cell biology|apoptosis|intracellular|caspases etc|caspases| neuroscience| cancer|invasion/microenvironment|cell death|apoptotic markers|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Caspase-3(CASP-3)

Caspase 3 is a caspase protein which interacts with Survivin, XIAP, CFLAR, Caspase 8, HCLS1, Deleted in Colorectal Cancer, TRAF3 and GroEL. This gene which is located at 4q35 encodes a protein that is a member of the cysteine-aspartic acid protease(caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes that undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7, and 9; and the protein itself is processed by caspases 8, 9, and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. And the caspase-3 activation in heart failure sequentially cleaves SRF and generates a truncated SRF that appears to function as a dominant-negative transcription factor. Additionally, the caspase-3 influence on bone mineral density should be considered in any in vivo application of caspase-3 inhibitors to the treatment of human disease. In erythroid precursors undergoing terminal differentiation, Hsp70 prevents active CASP3 from cleaving GATA1 and inducing apoptosis.

Anti-Caspase-3 (P10) Antibody Images

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Anti-Caspase-3 (P10) Antibody
Anti-Caspase-3(P10), PA1302-1, IHC(P)
IHC(P): Human Intestinal Cancer Tissue
Anti-Caspase-3 (P10) Antibody
Anti-Caspase-3(P10), PA1302-1, Western blotting
Lane 1: HELA Cell Lysate
Lane 2: SMMC Cell Lysate
Anti-Caspase-3 (P10) Antibody
Anti-Caspase-3(P10), PA1302-1, IHC(P)
IHC(P): Human Lung Cancer Tissue
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Publications

Qian C,, Gong L, Yang Z, Chen W, Chen Y, Xu Z, Wu B, Tang C, Gao F, Zeng W,. Bmc Cardiovasc Disord. 2015 Jun 26;15:59. Doi: 10.1186/S12872-015-0046-9. Diastolic Dysfunction In Spontaneous Type 2 Diabetes Rhesus Monkeys: A Study Using Echocardiogra...
Feng H, Yin Sh, Tang Az, Tan Sh. Neurochem Res. 2011 Jun;36(6):1108-15. Doi: 10.1007/S11064-011-0455-9. Epub 2011 Mar 31. Salicylate Initiates Apoptosis In The Spiral Ganglion Neuron Of Guinea Pig Cochlea By Activating Caspase-3.
Liang J, Chen H, Pan W, Xu C. Mol Med Rep. 2012 Jun;5(6):1419-22. Doi: 10.3892/Mmr.2012.854. Epub 2012 Apr 2. Puerarin Inhibits Caspase-3 Expression In Osteoblasts Of Diabetic Rats.
Jia Y, Lin J, Mi Y, Zhang C. Reprod Toxicol. 2011 May;31(4):477-85. Doi: 10.1016/J.Reprotox.2010.12.057. Epub 2011 Jan 22. Quercetin Attenuates Cadmium-Induced Oxidative Damage And Apoptosis In Granulosa Cells From Chicken Ovarian Follicles.
Zhang T, Su J, Wang K, Zhu T, Li X. Neurosci Lett. 2014 Sep 5;579:12-7. Doi: 10.1016/J.Neulet.2014.07.005. Epub 2014 Jul 12. Ursolic Acid Reduces Oxidative Stress To Alleviate Early Brain Injury Following Experimental Subarachnoid Hemorrhage.
Li Ys, Chen Bf, Li Xj, Zhang Wk, Tang Hb. Plos One. 2014 Sep 24;9(9):E107361. Doi: 10.1371/Journal.Pone.0107361. Ecollection 2014. Cytotoxicity Of Polyaniline Nanomaterial On Rat Celiac Macrophages In Vitro.
Zhang L, Yu S, Duan Z, Wang Q, Tian G, Tian Y, Zhao W, Wang H, Zhang C, Guo S, Liu Q, He G, Bian T, Chang J, Jin X, Cui D. Int J Mol Med. 2014 Jan;33(1):117-27. Doi: 10.3892/Ijmm.2013.1542. Epub 2013 Oct 30. Treatment Of Liver Cancer In Mice By Th...
Hu K, Xie Yy, Zhang C, Ouyang Ds, Long Hy, Sun Dn, Long Ll, Feng L, Li Y, Xiao B. Bmc Neurosci. 2012 Sep 22;13:115. Doi: 10.1186/1471-2202-13-115. Microrna Expression Profile Of The Hippocampus In A Rat Model Of Temporal Lobe Epilepsy And Mir-34A-...
Liu Nb, Peng T, Pan C, Yao Yy, Shen B, Leng J. World J Gastroenterol. 2005 Oct 28;11(40):6281-7. Overexpression Of Cyclooxygenase-2 In Human Hepg2, Bel-7402 And Smmc-7721 Hepatoma Cell Lines And Mechanism Of Cyclooxygenase-2 Selective Inhibitor Ce...
Li Z, Shao S, Xie S, Jiao F, Ma Y, Shi S. Ir J Med Sci. 2010 Jun;179(2):217-23. Doi: 10.1007/S11845-009-0418-1. Epub 2009 Dec 20. Silencing Of Ct120 By Antisense Oligonucleotides Could Inhibit The Lung Cancer Cells Growth.
Feng H, Yin Sh, Tang Az, Cai Hw, Chen P, Tan Sh, Xie Lh. Neurosci Lett. 2010 Jul 19;479(1):34-9. Doi: 10.1016/J.Neulet.2010.05.023. Epub 2010 May 15. Caspase-3 Activation In The Guinea Pig Cochlea Exposed To Salicylate.
Zhang S, Li H, Yang Sj. Acta Pharmacol Sin. 2010 Jun;31(6):671-8. Doi: 10.1038/Aps.2010.45. Epub 2010 May 10. Tribulosin Protects Rat Hearts From Ischemia/Reperfusion Injury.
Sun Mm, Li Jf, Guo Ll, Xiao Ht, Dong L, Wang F, Huang Fb, Cao D, Qin T, Yin Xh, Li Jm, Wang Sl. Oncogene. 2014 Apr 17;33(16):2075-86. Doi: 10.1038/Onc.2013.165. Epub 2013 May 13. Tgf-??1 Suppression Of Microrna-450B-5P Expression: A Novel Mechanism...
Oztanir Mn, Ciftci O, Cetin A, Aladag Ma. Neurol Sci. 2014 Sep;35(9):1393-9. Doi: 10.1007/S10072-014-1725-5. Epub 2014 Mar 28. Hesperidin Attenuates Oxidative And Neuronal Damage Caused By Global Cerebral Ischemia/Reperfusion In A C57Bl/J6 Mouse M...
Ciftci O, Oztanir Mn, Cetin A. Neurochem Res. 2014 Sep;39(9):1717-23. Doi: 10.1007/S11064-014-1365-4. Epub 2014 Jun 28. Neuroprotective Effects Of ??-Myrcene Following Global Cerebral Ischemia/Reperfusion-Mediated Oxidative And Neuronal Damage In A...
Chen Y, Li D, Xu Y, Zhang Y, Tao L, Li S, Jiang Y, Shen X. Evid Based Complement Alternat Med. 2014;2014:956824. Doi: 10.1155/2014/956824. Epub 2014 Dec 23. Essential Oils From Fructus A. Zerumbet Protect Human Aortic Endothelial Cells From Apopto...
Zhou X, Zhang Y, Li Y, Hao X, Liu X, Wang Y. Cancers (Basel). 2012 Dec 4;4(4):1318-32. Doi: 10.3390/Cancers4041318. Azithromycin Synergistically Enhances Anti-Proliferative Activity Of Vincristine In Cervical And Gastric Cancer Cells.
Li Q, Li Z, Xu Xy, Guo Yl, Du F. Int J Mol Sci. 2010 Nov 16;11(11):4580-90. Doi: 10.3390/Ijms11114580. Neuroprotective Properties Of Picroside Ii In A Rat Model Of Focal Cerebral Ischemia.
Chang C, Liu Sp, Fang Ch, He Rs, Wang Z, Zhu Yq, Jiang Sw. Oncol Lett. 2013 Sep;6(3):699-704. Epub 2013 Jul 8. Effects Of Matrine On The Proliferation Of Ht29 Human Colon Cancer Cells And Its Antitumor Mechanism.
Shu Hf, Yu Sx, Zhang Cq, Liu Sy, Wu Kf, Zang Zl, Yang H, Zhou Sw, Yin Q. Brain Dev. 2013 Mar;35(3):252-60. Doi: 10.1016/J.Braindev.2012.04.007. Epub 2012 May 28. Expression Of Trpv1 In Cortical Lesions From Patients With Tuberous Sclerosis Complex...
Wang Xf, Qu Xq, Zhang Tt, Zhang Jf. Exp Ther Med. 2015 Apr;9(4):1283-1291. Epub 2015 Feb 6. Testosterone Suppresses Ventricular Remodeling And Improves Left Ventricular Function In Rats Following Myocardial Infarction.
Li M, Peng J, Song Y, Liang H, Mei Y, Fang Y. J Huazhong Univ Sci Technolog Med Sci. 2012 Oct;32(5):746-9. Doi: 10.1007/S11596-012-1028-0. Epub 2012 Oct 18. Electro-Acupuncture Combined With Transcranial Magnetic Stimulation Improves Learning And ...
Wu Z, Wang L, Hou S. Neural Regen Res. 2012 Jul 5;7(19):1493-7. Doi: 10.3969/J.Issn.1673-5374.2012.19.008. Alpha B-Crystallin Improved Survival Of Retinal Ganglion Cells In A Rat Model Of Acute Ocular Hypertension.
Chen A, Dang T, Wang S, Tang N, Liu Y, Wu W. Sci China Life Sci. 2014 Jul;57(7):698-709. Doi: 10.1007/S11427-014-4680-8. Epub 2014 Jun 16. The In Vitro And In Vivo Anti-Tumor Effects Of Mtx-Fe3O 4-Plla-Peg-Plla Microspheres Prepared By Suspension-...
Yuan X, Sun Q, Ou Y, Wang S, Zhang W, Deng H, Wu X, Zhang L. Neural Regen Res. 2014 Apr 15;9(8):837-44. Doi: 10.4103/1673-5374.131600. Apoptosis Is An Obstacle To The Differentiation Of Adipose-Derived Stromal Cells Into Astrocytes.
Chen S, Peng H, Rowat A, Gao F, Zhang Z, Wang P, Zhang W, Wang X, Qu L. Brain Res. 2015 Aug 27;1618:205-11. Doi: 10.1016/J.Brainres.2015.05.027. Epub 2015 May 30. The Effect Of Concentration And Duration Of Normobaric Oxygen In Reducing Caspase-3 ...
Dai C, Li J, Tang S, Li J, Xiao X. Antimicrob Agents Chemother. 2014 Jul;58(7):4075-85. Doi: 10.1128/Aac.00070-14. Epub 2014 May 5. Colistin-Induced Nephrotoxicity In Mice Involves The Mitochondrial, Death Receptor, And Endoplasmic Reticulum Pathw...
Ji J, Yan X, Li Z, Lai Z, Liu J. Biomed Pharmacother. 2015 Feb;69:311-6. Doi: 10.1016/J.Biopha.2014.12.020. Epub 2014 Dec 19. Therapeutic Effects Of Intrathecal Versus Intravenous Monosialoganglioside Against Bupivacaine-Induced Spinal Neurotoxici...
Lin Hd, He Cq, Luo Ql, Zhang Jl, Zeng Dx. Rheumatol Int. 2012 Mar;32(3):759-66. Doi: 10.1007/S00296-010-1629-5. Epub 2010 Dec 28. The Effect Of Low-Level Laser To Apoptosis Of Chondrocyte And Caspases Expression, Including Caspase-8 And Caspase-3 ...
Guo H, Luo Q, Zhang J, Lin H, Xia L, He C. Joint Bone Spine. 2011 Dec;78(6):604-10. Doi: 10.1016/J.Jbspin.2011.01.009. Epub 2011 Mar 11. Comparing Different Physical Factors On Serum Tnf-?? Levels, Chondrocyte Apoptosis, Caspase-3 And Caspase-8 Exp...
Zeng D, Luo Q, Lin H, Zhang J, He C. Rheumatol Int. 2012 Dec;32(12):3771-7. Doi: 10.1007/S00296-011-2196-0. Epub 2011 Dec 14. The Effect Of Therapeutic Ultrasound To Apoptosis Of Chondrocyte And Caspase-3 And Caspase-8 Expression In Rabbit Surgery...
Shan S, Li Z, Newton Ip, Zhao C, Li Z, Guo M. Toxicol Lett. 2014 Jun 5;227(2):129-38. Doi: 10.1016/J.Toxlet.2014.03.008. Epub 2014 Mar 28. A Novel Protein Extracted From Foxtail Millet Bran Displays Anti-Carcinogenic Effects In Human Colon Cancer ...
Zhao X, Chen Q, Li Y, Tang H, Liu W, Yang X. Eur J Pharm Biopharm. 2015 Jun;93:27-36. Doi: 10.1016/J.Ejpb.2015.03.003. Epub 2015 Mar 11. Doxorubicin And Curcumin Co-Delivery By Lipid Nanoparticles For Enhanced Treatment Of Diethylnitrosamine-Induc...
Lin S, Lin K, Li W, Zhou X, Huang T. Endocrine. 2010 Jun;37(3):460-6. Doi: 10.1007/S12020-010-9337-1. Epub 2010 Apr 21. Maternal Diabetes Increases Apoptosis In Mice Oocytes, Not 2-Cell Embryos.
Li Wz, Li Wp, Huang Dk, Kan Hw, Wang X, Wu Wy, Yin Yy, Yao Yy. Behav Brain Res. 2012 Feb 1;227(1):142-9. Doi: 10.1016/J.Bbr.2011.10.038. Epub 2011 Oct 29. Dexamethasone And A????????-?????? Accelerate Learning And Memory Impairments Due To Elevate Amyloid ...
Lu Y, Yuan X, Ou Y, Cai Y, Wang S, Sun Q, Zhang W. Neural Regen Res. 2012 Jun 5;7(16):1205-12. Doi: 10.3969/J.Issn.1673-5374.2012.16.001. Autophagy And Apoptosis During Adult Adipose-Derived Stromal Cells Differentiation Into Neuron-Like Cells In ...
Chu Sh, Ma Yb, Feng Df, Zhang H, Qiu Jh, Zhu Za. Oncol Lett. 2011 Nov;2(6):1139-1142. Epub 2011 Aug 4. Elevated Expression Of Solute Carrier Family 22 Member 18 Increases The Sensitivity Of U251 Glioma Cells To Bcnu.
Zhao B, Li X. Oncol Rep. 2014 Jun;31(6):2769-75. Doi: 10.3892/Or.2014.3126. Epub 2014 Apr 3. Altholactone Induces Reactive Oxygen Species-Mediated Apoptosis In Bladder Cancer T24 Cells Through Mitochondrial Dysfunction, Mapk-P38 Activation And Akt...
Zhan W, Hu X, Yi J, An Q, Huang X. Mol Med Rep. 2015 Jun;11(6):4142-8. Doi: 10.3892/Mmr.2015.3326. Epub 2015 Feb 10. Inhibitory Activity Of Apogossypol In Human Prostate Cancer In Vitro And In Vivo.
Kan S, Zhou H, Jin C, Yang H. Int J Clin Exp Med. 2015 Mar 15;8(3):3258-70. Ecollection 2015. Effects Of Pdtc On Nf-??b Expression And Apoptosis In Rats With Severe Acute Pancreatitis-Associated Lung Injury.
Wang Jm, Xiao Bl, Zheng Jw, Chen Hb, Zou Sq. World J Gastroenterol. 2007 Jun 21;13(23):3171-5. Effect Of Targeted Magnetic Nanoparticles Containing 5-Fu On Expression Of Bcl-2, Bax And Caspase 3 In Nude Mice With Transplanted Human Liver Cancer.
Meng Qt, Xia Zy, Luo T, Cao C, Zhao B, Wu Y, Wu X, Chen X. Saudi Med J. 2012 Feb;33(2):139-45. Ligustrazine Attenuates Acute Lung Injury Induced By Blunt Chest Trauma.
Zhou Y, Li W, Xu Q, Huang Y. J Exp Clin Cancer Res. 2010 Oct 4;29:131. Doi: 10.1186/1756-9966-29-131. Elevated Expression Of Dickkopf-1 Increases The Sensitivity Of Human Glioma Cell Line Shg44 To Bcnu.
Rasul A, Yu B, Khan M, Zhang K, Iqbal F, Ma T, Yang H. Int J Oncol. 2012 Apr;40(4):1153-61. Doi: 10.3892/Ijo.2011.1277. Epub 2011 Nov 30. Magnolol, A Natural Compound, Induces Apoptosis Of Sgc-7901 Human Gastric Adenocarcinoma Cells Vi...
Peng X, Chen K, Chen J, Fang J, Cui H, Zuo Z, Deng J, Chen Z, Geng Y, Lai W. Environ Toxicol. 2015 Mar 4. Doi: 10.1002/Tox.22120. [Epub Ahead Of Print] Aflatoxin B1 Affects Apoptosis And Expression Of Bax, Bcl-2, And Caspase-3 In Thymus And Bursa ...
Chen J, Li X, Qiu J, You H, Zhang Q, Dong G, Zuo Y. Cell Biochem Biophys. 2013;67(3):1015-9. Doi: 10.1007/S12013-013-9597-5. Kinetics Of Apoptosis And Expression Of Apoptosis-Related Proteins In Rat Ca3 Hippocampus Cells After Experimental Diffuse...
Liu Y, Zhou Y, Wang Y, Geng D, Liu J. Obes Surg. 2011 Sep;21(9):1424-31. Doi: 10.1007/S11695-011-0388-Z. Roux-En-Y Gastric Bypass-Induced Improvement Of Glucose Tolerance And Insulin Resistance In Type 2 Diabetic Rats Are Mediated By Glucagon-Like...
Yang Y, Li Zh, Liu H, Shi Wd, Zhang J. Chin J Integr Med. 2015 Jun;21(6):423-30. Doi: 10.1007/S11655-014-1752-3. Epub 2014 May 10. Inhibitory Effect Of Tetramethylpyrazine Preconditioning On Overload Training-Induced Myocardial Apoptosis In Rats.
Song Gh, Wang Rl, Chen Zy, Zhang B, Wang Hl, Liu Ml, Gao Jp, Yan Xy. J Physiol Biochem. 2014 Sep;70(3):761-8. Doi: 10.1007/S13105-014-0344-1. Epub 2014 Jul 30. Toxic Effects Of Sodium Fluoride On Cell Proliferation And Apoptosis Of Leydig Cells Fr...
Chen K, Shu G, Peng X, Fang J, Cui H, Chen J, Wang F, Chen Z, Zuo Z, Deng J, Geng Y, Lai W. Food Chem Toxicol. 2013 Sep;59:446-54. Doi: 10.1016/J.Fct.2013.06.032. Epub 2013 Jun 28. Protective Role Of Sodium Selenite On Histopathological Lesions, D...
Mao Z, Song Z, Li G, Lv W, Zhao X, Li B, Feng X, Chen Y. Neural Regen Res. 2013 Jan 15;8(2):133-42. Doi: 10.3969/J.Issn.1673-5374.2013.02.005. 8-Hydroxy-2-(Di-N-Propylamino)Tetralin Intervenes With Neural Cell Apoptosis Following Diffuse Axonal In...
Yan Y, Liang B, Zhang J, Liu Y, Bu X. Mol Med Rep. 2015 Jan;11(1):317-26. Doi: 10.3892/Mmr.2014.2657. Epub 2014 Oct 15. Apoptotic Induction Of Lung Adenocarcinoma A549 Cells Infected By Recombinant Rvg Newcastle Disease Virus (Rl-Rvg) In Vitro.
Li X, Wang L, Wang Z. Phys Med. 2015 Jun;31(4):352-9. Doi: 10.1016/J.Ejmp.2015.02.004. Epub 2015 Feb 19. Radioprotective Activity Of Neutral Polysaccharides Isolated From The Fruiting Bodies Of Hohenbuehelia Serotina.
Wei T, Tian W, Yan H, Shao G, Xie G. Cell Mol Neurobiol. 2014 Nov;34(8):1165-73. Doi: 10.1007/S10571-014-0091-4. Epub 2014 Aug 22. Protective Effects Of Phillyrin On H2O 2-Induced Oxidative Stress And Apoptosis In Pc12 Cells.
Ding H, Wang H, Zhao Y, Sun D, Zhai X. Cell Mol Neurobiol. 2015 Jul;35(5):623-32. Doi: 10.1007/S10571-015-0156-Z. Epub 2015 Jan 18. Protective Effects Of Baicalin On A??????????????-Induced Learning And Memory Deficit, Oxidative Stress, And Apoptosis In Rat.
Xu S, Liu J, Zhang Y, Wang C, Wang J, Yang Y, Huo J, Sun W. Neural Regen Res. 2012 Jun 15;7(17):1318-24. Doi: 10.3969/J.Issn.1673-5374.2012.17.006. Apoptosis-Related Protein Expression In Rabbits With Blast Brain Injury Following Early Hyperbaric ...
Khan M, Li T, Ahmad Khan Mk, Rasul A, Nawaz F, Sun M, Zheng Y, Ma T. Biomed Res Int. 2013;2013:719858. Doi: 10.1155/2013/719858. Epub 2012 Dec 27. Alantolactone Induces Apoptosis In Hepg2 Cells Through Gsh Depletion, Inhibition Of Stat3 Activation...
Tian W, Wei T, Li B, Wang Z, Zhang N, Xie G. Plos One. 2014 May 6;9(5):E96775. Doi: 10.1371/Journal.Pone.0096775. Ecollection 2014. Pathway Of Programmed Cell Death And Oxidative Stress Induced By ??-Hydroxybutyrate In Dairy Cow Abomasum Smooth Mus...
Dong Hy, Wang Zf. Chin J Cancer Res. 2014 Apr;26(2):200-7. Doi: 10.3978/J.Issn.1000-9604.2014.04.07. Antitumor Effects Of Artesunate On Human Breast Carcinoma Mcf-7 Cells And Igf-Ir Expression In Nude Mice Xenografts.
Huang Kf, Huang Xp, Xiao Gq, Yang Hy, Lin Js, Diao Y. Biomed Pharmacother. 2014 May;68(4):455-61. Doi: 10.1016/J.Biopha.2014.03.005. Epub 2014 Mar 18. Kallistatin, A Novel Anti-Angiogenesis Agent, Inhibits Angiogenesis Via Inhibition Of The Nf-??b ...
Sun Y, Yang J, Wang Lz, Sun Lr, Dong Q. Hum Exp Toxicol. 2014 Aug;33(8):855-62. Doi: 10.1177/0960327113511475. Epub 2013 Nov 25. Crocin Attenuates Cisplatin-Induced Liver Injury In The Mice.
Fu Y, Zhao Z, Wu Y, Wu K, Xu X, Liu Y, Tong C. J Ethnopharmacol. 2012 Jan 6;139(1):26-33. Doi: 10.1016/J.Jep.2011.08.077. Epub 2011 Nov 10. Therapeutic Mechanisms Of Tongmai Dasheng Tablet On Tripterygium Glycosides Induced Rat Model For Premature...
Song D, Yue W, Li Z, Li J, Zhao J, Zhang N. Onco Targets Ther. 2014 Sep 30;7:1801-10. Doi: 10.2147/Ott.S52426. Ecollection 2014. Study Of The Mechanism Of Sonodynamic Therapy In A Rat Glioma Model.
Xia L, Luo Ql, Lin Hd, Zhang Jl, Guo H, He Cq. Rheumatol Int. 2012 Sep;32(9):2847-56. Doi: 10.1007/S00296-011-2080-Y. Epub 2011 Sep 1. The Effect Of Different Treatment Time Of Millimeter Wave On Chondrocyte Apoptosis, Caspase-3, Caspase-8, And Mm...
Jiang H, Li J, Zhou T, Wang C, Zhang H, Wang H. Int J Mol Med. 2014 May;33(5):1298-304. Doi: 10.3892/Ijmm.2014.1684. Epub 2014 Mar 6. Colistin-Induced Apoptosis In Pc12 Cells: Involvement Of The Mitochondrial Apoptotic And Death Receptor Pathways.
Wu Yh, Hu Sq, Liu J, Cao Hc, Xu W, Li Yj, Li Lj. Int J Mol Med. 2014 Jun;33(6):1498-506. Doi: 10.3892/Ijmm.2014.1730. Epub 2014 Apr 7. Nature And Mechanisms Of Hepatocyte Apoptosis Induced By D-Galactosamine/Lipopolysaccharide Challenge In Mice.
Ge C, Yu M, Petitte Jn, Zhang C. Biol Reprod. 2009 Mar;80(3):528-36. Doi: 10.1095/Biolreprod.108.072728. Epub 2008 Nov 12. Epidermal Growth Factor-Induced Proliferation Of Chicken Primordial Germ Cells: Involvement Of Calcium/Protein Kinase C And ...

FAQs

Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
A: You can find the immunogen sequence under "Immunogen" and clonality in the product name.
Q: What is the expected band size? Why is it different than the observed band size?
A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands

3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.

4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
A: Check our protocols under the tech support tab.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to caspase 3 antibody, casp3 antibody, caspase3 antibody