Anti-Caspase-8 Monoclonal Antibody (5F7)

Boster Bio Anti-Caspase-8 Monoclonal Antibody (5F7) catalog # M00042-3. Tested in IP, IHC, WB applications. This antibody reacts with Human. Cited in 3 publication(s).

Product Info Summary

SKU: M00042-3
Size: 100µg
Reactive Species: Human
Host: Mouse
Application: IP, IHC, WB

Product Name

Anti-Caspase-8 Monoclonal Antibody (5F7)

See all Caspase-8 products

SKU/Catalog Number

M00042-3

Size

100µg

Form

Liquid. In PBS, pH 7.2, containing 50% glycerol.

Description

Boster Bio Anti-Caspase-8 Monoclonal Antibody (5F7) catalog # M00042-3. Tested in IP, IHC, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Caspase-8 Monoclonal Antibody (5F7) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00042-3)

Host

Mouse

Contents

Liquid. In PBS, pH 7.2, containing 50% glycerol.

Clonality

Monoclonal

Clone Number

5F7

Immunogen

C-terminal fragment of recombinant human caspase-8 (amino acids 176-460) .

Reactive Species

M00042-3 is reactive to CASP8 in Human

Applications

M00042-3 is guaranteed for IP, IHC, WB Boster Guarantee

*Blocking peptide can be purchased at $150. Contact us for more information.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Immunoprecipitation (1:50)
Western Blot (1µg/ml, ECL)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.

Validation Images & Assay Conditions

Gene/Protein Information For CASP8 (Source: Uniprot.Org, NCBI)

Uniprot ID

Q14790

Gene ID

841

Gene Name

CASP8

Full Name

Caspase-8

Weight

55.391kDa

Superfamily

peptidase C14A family

Alternative Names

AIS; androgen receptor; CASP8; Caspase8; Caspase-8; DHTRTFM; Dihydrotestosterone receptorHYSP1; HUMARA; Mch5; NR3C4KD; Nuclear receptor subfamily 3 group C member 4; SMAX1SBMA; spinal and bulbar muscular atrophy CASP8 ALPS2B, CAP4, Casp-8, FLICE, MACH, MCH5 caspase 8 caspase-8|FADD-homologous ICE/CED-3-like protease|FADD-like ICE|ICE-like apoptotic protease 5|MACH-alpha-1/2/3 protein|MACH-beta-1/2/3/4 protein|MORT1-associated ced-3 homolog|apoptotic cysteine protease|apoptotic protease Mch-5|caspase 8, apoptosis-related cysteine peptidase|caspase 8, apoptosis-related cysteine protease

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on CASP8, check out the CASP8 Infographic

CASP8 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for CASP8: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

M00042-3 has been cited in 3 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Inhibition of proliferation of prostate cancer cell line, PC-3, in vitro and in vivo using (%u2212)-gossypol

Protective effect of RIP and c-FLIP in preventing liver cancer cell apoptosis induced by TRAIL

Croton Tiglium Extract Induces Apoptosis via Bax/Bcl-2 Pathways in Human Lung Cancer A549 Cells

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15 Customer Q&As for Anti-Caspase-8 Monoclonal Antibody (5F7)

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for small intestine peyer's patch using anti-Caspase-8 Monoclonal antibody (5F7) M00042-3. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2020-03-10

Answer

Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-03-10

Question

Our lab used your anti-Caspase-8 Monoclonal antibody (5F7) for IP on t-cell in a previous experiment. I am using human, and We want to use the antibody for IHC next. Our lab want to know about examining t-cell as well as leukocyte in our next experiment. Could give a recommendation on which antibody would work the best for IHC?

R. Patel

Verified customer

Asked: 2020-02-12

Answer

I have checked the website and datasheets of our anti-Caspase-8 Monoclonal antibody (5F7) and it appears that M00042-3 has been tested on human in both IP and IHC. Thus M00042-3 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2020-02-12

Question

Would M00042-3 anti-Caspase-8 Monoclonal antibody (5F7) work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2020-01-27

Answer

It shows on the product datasheet, M00042-3 anti-Caspase-8 Monoclonal antibody (5F7) as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2020-01-27

Question

We have been able to see staining in human b-cell thymus. Do you have any suggestions? Is anti-Caspase-8 Monoclonal antibody (5F7) supposed to stain b-cell thymus positively?

Verified Customer

Verified customer

Asked: 2019-07-09

Answer

From what I have seen in literature b-cell thymus does express CASP8. From what I have seen in Uniprot.org, CASP8 is expressed in small intestine peyer's patch, b-cell thymus, t-cell, leukocyte, among other tissues. Regarding which tissues have CASP8 expression, here are a few articles citing expression in various tissues:
B-cell, and Thymus, Pubmed ID: 8681376
Leukocyte, Pubmed ID: 12010809, 15489334
T-cell, Pubmed ID: 8755496

Boster Scientific Support

Answered: 2019-07-09

Question

I have a question about product M00042-3, anti-Caspase-8 Monoclonal antibody (5F7) . I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-06-24

Answer

We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00042-3 anti-Caspase-8 Monoclonal antibody (5F7) , we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-06-24

Question

I see that the anti-Caspase-8 Monoclonal antibody (5F7) M00042-3 works with IHC, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-06-04

Answer

You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-06-04

Question

See attached the WB image, lot number and protocol we used for small intestine peyer's patch using anti-Caspase-8 Monoclonal antibody (5F7) M00042-3. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-06-04

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-06-04

Question

I was wanting to use your anti-Caspase-8 Monoclonal antibody (5F7) for IHC for human small intestine peyer's patch on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human small intestine peyer's patch identification?

Verified Customer

Verified customer

Asked: 2017-07-20

Answer

It shows on the product datasheet, M00042-3 anti-Caspase-8 Monoclonal antibody (5F7) has been validated for IP, IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human small intestine peyer's patch in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2017-07-20

Question

My colleagues were happy with the WB result of your anti-Caspase-8 Monoclonal antibody (5F7) . However we have observed positive staining in b-cell thymus cytoplasm. using this antibody. Is that expected? Could you tell me where is CASP8 supposed to be expressed?

J. Jones

Verified customer

Asked: 2015-05-25

Answer

According to literature, b-cell thymus does express CASP8. Generally CASP8 expresses in cytoplasm. Regarding which tissues have CASP8 expression, here are a few articles citing expression in various tissues:
B-cell, and Thymus, Pubmed ID: 8681376
Leukocyte, Pubmed ID: 12010809, 15489334
T-cell, Pubmed ID: 8755496

Boster Scientific Support

Answered: 2015-05-25

Question

Would anti-Caspase-8 Monoclonal antibody (5F7) M00042-3 work for IHC with small intestine peyer's patch?

A. Moore

Verified customer

Asked: 2014-09-12

Answer

According to the expression profile of small intestine peyer's patch, CASP8 is highly expressed in small intestine peyer's patch. So, it is likely that anti-Caspase-8 Monoclonal antibody (5F7) M00042-3 will work for IHC with small intestine peyer's patch.

Boster Scientific Support

Answered: 2014-09-12

Question

Is there a BSA free version of anti-Caspase-8 Monoclonal antibody (5F7) M00042-3 available?

V. Mitchell

Verified customer

Asked: 2014-09-08

Answer

Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-Caspase-8 Monoclonal antibody (5F7) M00042-3 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2014-09-08

Question

Is this M00042-3 anti-Caspase-8 Monoclonal antibody (5F7) reactive to the isotypes of CASP8?

G. Jha

Verified customer

Asked: 2014-07-08

Answer

The immunogen of M00042-3 anti-Caspase-8 Monoclonal antibody (5F7) is C-terminal fragment of recombinant human caspase-8 (amino acids 176-460) . Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2014-07-08

Question

We are interested in to test anti-Caspase-8 Monoclonal antibody (5F7) M00042-3 on human small intestine peyer's patch for research purposes, then I may be interested in using anti-Caspase-8 Monoclonal antibody (5F7) M00042-3 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

C. Taylor

Verified customer

Asked: 2013-07-18

Answer

The products we sell, including anti-Caspase-8 Monoclonal antibody (5F7) M00042-3, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2013-07-18

Question

Is a blocking peptide available for product anti-Caspase-8 Monoclonal antibody (5F7) (M00042-3)?

T. Krishna

Verified customer

Asked: 2013-03-20

Answer

We do provide the blocking peptide for product anti-Caspase-8 Monoclonal antibody (5F7) (M00042-3). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2013-03-20

Question

We are currently using anti-Caspase-8 Monoclonal antibody (5F7) M00042-3 for human tissue, and we are well pleased with the IP results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on primate tissues as well?

P. Patel

Verified customer

Asked: 2013-03-04

Answer

The anti-Caspase-8 Monoclonal antibody (5F7) (M00042-3) has not been tested for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2013-03-04

Size

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Ships directly to USA and Canada. For other regions, visit our distributors page

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M00042-3
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$388.00

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