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SKU:PA1524
Clonality:Polyclonal
Application:IHC-P, WB
Price: $200.00
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Overview

Product Name Anti-Caspase-8(P10) Antibody
Description Rabbit IgG polyclonal antibody for Caspase-8(CASP8) detection. Tested with WB, IHC-P in Human.
Cite This Product Anti-Caspase-8(P10) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1524)
Replacement Item This antibody may replace the following items: sc-136160|sc-166320|sc-166596|sc-393776|sc-5263|sc-5276|sc-56070|sc-56071|sc-56072|sc-6133|sc-6134|sc-6135|sc-6136|sc-70501|sc-70502|sc-7108|sc-7111|sc-73526|sc-7890|sc-81656|sc-81657|sc-81661|sc-81662|sc-8346|sc-8347 from Santa Cruz Biotechnology.
Host Rabbit
Isotype N/A
Validated Species Human
Application IHC-P, WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen A synthetic peptide corresponding to a sequence at the C-terminus of human Caspase-8(P10)(458-473aa TILTEVNYEVSNKDDK).
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Polyclonal
Form Lyophilized
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
Concentration Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Immunogen affinity purified.
Isotype N/A

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name CASP8
Protein Name Caspase-8(CASP-8)
Molecular Weight 55391 MW
Protein Function Most upstream protease of the activation cascade of caspases responsible for the TNFRSF6/FAS mediated and TNFRSF1A induced cell death. Binding to the adapter molecule FADD recruits it to either receptor. The resulting aggregate called death- inducing signaling complex (DISC) performs CASP8 proteolytic activation. The active dimeric enzyme is then liberated from the DISC and free to activate downstream apoptotic proteases. Proteolytic fragments of the N-terminal propeptide (termed CAP3, CAP5 and CAP6) are likely retained in the DISC. Cleaves and activates CASP3, CASP4, CASP6, CASP7, CASP9 and CASP10. May participate in the GZMB apoptotic pathways. Cleaves ADPRT. Hydrolyzes the small-molecule substrate, Ac-Asp-Glu-Val-Asp-

-AMC. Likely target for the cowpox virus CRMA death inhibitory protein. Isoform 5, isoform 6, isoform 7 and isoform 8 lack the catalytic site and may interfere with the pro-apoptotic activity of the complex. .
Tissue Specificity Isoform 1, isoform 5 and isoform 7 are expressed in a wide variety of tissues. Highest expression in peripheral blood leukocytes, spleen, thymus and liver. Barely detectable in brain, testis and skeletal muscle.
Sequence Similarities Belongs to the peptidase C14A family.
Subcellular Localization Cytoplasm.
Uniprot ID Q14790
Alternative Names Caspase-8;CASP-8;3.4.22.61;Apoptotic cysteine protease;Apoptotic protease Mch-5;CAP4;FADD-homologous ICE/ced-3-like protease;FADD-like ICE;FLICE;ICE-like apoptotic protease 5;MORT1-associated ced-3 homolog;MACH;Caspase-8 subunit p18;Caspase-8 subunit p10;CASP8;MCH5;
Research Areas CASP8|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Caspase-8(CASP-8)

Caspase 8 is a caspase protein. It most likely acts upon caspase 3. This gene encodes a member of the cysteine-aspartic acid protease(caspase) family. The human CASP8 gene, whose product is also known as caspase 8 and FLICE, encodes an interleukin-1beta converting enzyme(ICE)-related cysteine protease that is activated by the engagement of several different death receptors. Caspase 8 is immediately recruited to the Fas receptor once it oligomerizes, and its protease activity is crucial for the apoptotic response generated by the resulting death-inducing signaling complex(DISC). This gene contains at least 11 exons spanning approximately 30kb on human chromosome band 2q33-34. This region of human chromosome 2 was previously reported as the location of the CASP10 gene, whose product is closely related to caspase 8.Caspase-8 deficiency in humans is compatible with normal development and shows that caspase-8 has a postnatal role in immune activation of naive lymphocytes.

Anti-Caspase-8(P10) Antibody Images

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Anti-Caspase-8(P10) Antibody
Anti-Caspase-8(P10) antibody, PA1524, Western blotting
WB: HELA Cell Lysate
Anti-Caspase-8(P10) Antibody
Anti-Caspase-8(P10) antibody, PA1524, IHC(P)
IHC(P): Human Mammary Cancer Tissue
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Publications

Liu N, Chen T, Wang X, Yang D, Xue B, Zhu H. Febs Lett. 2015 Apr 2;589(8):897-903. Doi: 10.1016/J.Febslet.2015.02.026. Epub 2015 Mar 3. Msi1 Confers Resistance To Trail By Activating Erk In Liver Cancer Cells.
Lin Hd, He Cq, Luo Ql, Zhang Jl, Zeng Dx. Rheumatol Int. 2012 Mar;32(3):759-66. Doi: 10.1007/S00296-010-1629-5. Epub 2010 Dec 28. The Effect Of Low-Level Laser To Apoptosis Of Chondrocyte And Caspases Expression, Including Caspase-8 And Caspase-3 ...
Guo H, Luo Q, Zhang J, Lin H, Xia L, He C. Joint Bone Spine. 2011 Dec;78(6):604-10. Doi: 10.1016/J.Jbspin.2011.01.009. Epub 2011 Mar 11. Comparing Different Physical Factors On Serum Tnf-?? Levels, Chondrocyte Apoptosis, Caspase-3 And Caspase-8 Exp...
Zeng D, Luo Q, Lin H, Zhang J, He C. Rheumatol Int. 2012 Dec;32(12):3771-7. Doi: 10.1007/S00296-011-2196-0. Epub 2011 Dec 14. The Effect Of Therapeutic Ultrasound To Apoptosis Of Chondrocyte And Caspase-3 And Caspase-8 Expression In Rabbit Surgery...
Shan S, Li Z, Newton Ip, Zhao C, Li Z, Guo M. Toxicol Lett. 2014 Jun 5;227(2):129-38. Doi: 10.1016/J.Toxlet.2014.03.008. Epub 2014 Mar 28. A Novel Protein Extracted From Foxtail Millet Bran Displays Anti-Carcinogenic Effects In Human Colon Cancer ...
Zhan W, Hu X, Yi J, An Q, Huang X. Mol Med Rep. 2015 Jun;11(6):4142-8. Doi: 10.3892/Mmr.2015.3326. Epub 2015 Feb 10. Inhibitory Activity Of Apogossypol In Human Prostate Cancer In Vitro And In Vivo.
Yan Y, Liang B, Zhang J, Liu Y, Bu X. Mol Med Rep. 2015 Jan;11(1):317-26. Doi: 10.3892/Mmr.2014.2657. Epub 2014 Oct 15. Apoptotic Induction Of Lung Adenocarcinoma A549 Cells Infected By Recombinant Rvg Newcastle Disease Virus (Rl-Rvg) In Vitro.
Xia L, Luo Ql, Lin Hd, Zhang Jl, Guo H, He Cq. Rheumatol Int. 2012 Sep;32(9):2847-56. Doi: 10.1007/S00296-011-2080-Y. Epub 2011 Sep 1. The Effect Of Different Treatment Time Of Millimeter Wave On Chondrocyte Apoptosis, Caspase-3, Caspase-8, And Mm...
Jiang H, Li J, Zhou T, Wang C, Zhang H, Wang H. Int J Mol Med. 2014 May;33(5):1298-304. Doi: 10.3892/Ijmm.2014.1684. Epub 2014 Mar 6. Colistin-Induced Apoptosis In Pc12 Cells: Involvement Of The Mitochondrial Apoptotic And Death Receptor Pathways.

FAQs

Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
A: You can find the immunogen sequence under "Immunogen" and clonality in the product name.
Q: What is the expected band size? Why is it different than the observed band size?
A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands

3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.

4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
A: Check our protocols under the tech support tab.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to caspase 8 antibody, caspase8 antibody