SKU M00004
Size 100ug/vial
Reactivity Bovine, Canine, Human, Mouse, Rat
Clonality Monoclonal
Clone Number 15B8
Host Mouse
Applications IHC

Overview

Product Name Anti-Catenin, beta (p120) Monoclonal Antibody
SKU/Catalog Number M00004
Storage & Handling [storage]
Size 100ug/vial
Description Anti-Catenin, beta (p120) Antibody
Cite This Product Anti-Catenin, beta (p120) Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00004)
Host Mouse
Contents/Buffer Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Form Liquid
Immunogen Recombinant chicken ?-catenin
Reactivity Bovine, Canine, Human, Mouse, Rat

Assay Details

Assay Dilutions Overview

Concentration: 0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
Immunohistology (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
Immunofluorescence (1-2ug/ml)

Images And Assay Conditions

/C/T/CTNNB1 primary antibodies M00004 IHC testing 1.jpg

Formalin-fixed, paraffin-embedded human Tonsil stained withBeta-Catenin (p120) Monoclonal Antibody (15B8).

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id P35222
Gene Name CTNNB1
Protein Name Catenin beta-1
Tissue Specificity Expressed in several hair follicle cell types: basal and peripheral matrix cells, and cells of the outer and inner root sheaths. Expressed in colon. Present in cortical neurons (at protein level). .
Alternative Names Catenin beta-1;Beta-catenin;CTNNB1;CTNNB;OK/SW-cl.35, PRO2286;
Subcellular Localization Cytoplasm. Nucleus . Cytoplasm, cytoskeleton. Cell junction, adherens junction. Cell junction. Cell membrane . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle pole. Colocalized with RAPGEF2 and TJP1 at cell-cell contacts (By similarity). Cytoplasmic when it is unstabilized (high level of phosphorylation) or bound to CDH1. Translocates to the nucleus when it is stabilized (low level of phosphorylation). Interaction with GLIS2 and MUC1 promotes nuclear translocation. Interaction with EMD inhibits nuclear localization. The majority of beta-catenin is localized to the cell membrane. In interphase, colocalizes with CROCC between CEP250 puncta at the proximal end of centrioles, and this localization is dependent on CROCC and CEP250. In mitosis, when NEK2 activity increases, it localizes to centrosomes at spindle poles independent of CROCC. Colocalizes with CDK5 in the cell-cell contacts and plasma membrane of undifferentiated and differentiated neuroblastoma cells. .
Molecular Weight 85497 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function Key downstream component of the canonical Wnt signaling pathway. In the absence of Wnt, forms a complex with AXIN1, AXIN2, APC, CSNK1A1 and GSK3B that promotes phosphorylation on N-terminal Ser and Thr residues and ubiquitination of CTNNB1 via BTRC and its subsequent degradation by the proteasome. In the presence of Wnt ligand, CTNNB1 is not ubiquitinated and accumulates in the nucleus, where it acts as a coactivator for transcription factors of the TCF/LEF family, leading to activate Wnt responsive genes. Involved in the regulation of cell adhesion. Acts as a negative regulator of centrosome cohesion. Involved in the CDK2/PTPN6/CTNNB1/CEACAM1 pathway of insulin internalization. Blocks anoikis of malignant kidney and intestinal epithelial cells and promotes their anchorage-independent growth by down-regulating DAPK2. Disrupts PML function and PML-NB formation by inhibiting RANBP2-mediated sumoylation of PML (PubMed:17524503, PubMed:18077326, PubMed:18086858, PubMed:18957423, PubMed:21262353, PubMed:22647378, PubMed:22699938, PubMed:22155184). Promotes neurogenesis by maintaining sympathetic neuroblasts within the cell cycle (By similarity). .
Research Areas Actin Etc, Atherosclerosis, Cadherins, Cancer, Cardiovascular, Cell Adhesion, Cytoplasmic, Cytoskeleton, Cytoskeleton / Ecm, Invasion/Microenvironment, Microfilaments, Neurogenesis, Neurology Process, Neuroscience, Signal Transduction, Signaling Pathways, Stem Cells, Vascular Inflammation

*You can search these to find other products in these research areas.

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Order Product (M00004)

Promotion:

Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
Option Price
20ug sample size $99
100ug $299
100ug+Free HRP Secondary BA1050 $299
100ug+Free Biotin Secondary BA1001 $299

USD $299

Ships in 7-10 business days.

Troubleshooting

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Publications

Dark Aberrant Crypt Foci with activated Wnt pathway are related to tumorigenesis in the colon of AOM-treated rat
Anti-cancer drug 3, 3%u2032-diindolylmethane activates Wnt4 signaling to enhance gastric cancer cell stemness and tumorigenesis
Oestrogen and parathyroid hormone alleviate lumbar intervertebral disc degeneration in ovariectomized rats and enhance Wnt/?-catenin pathway activity
Targeted p53 activation by saRNA suppresses human bladder cancer cells growth and metastasis
Wnt/?-catenin pathway is required for epithelial to mesenchymal transition in CXCL12 over expressed breast cancer cells
Curcumin alleviates glucocorticoid-induced osteoporosis through the regulation of the Wnt signaling pathway
Prolonged overexpression of Wnt10b induces epidermal keratinocyte transformation through activating EGF pathway
Celastrol Ameliorates Ulcerative Colitis-Related Colorectal Cancer in Mice via Suppressing Inflammatory Responses and Epithelial-Mesenchymal Transition
Combination of lithium chloride and pEGFP-N1-BmK CT effectively decreases proliferation and migration of C6 glioma cells
Modulating hair follicle size with?Wnt10b-DKK1?pair during hair regeneration
Li C, Liu J, Zhang Q, Cui K, Ge Q, Wang C, Chen Z. Int J Oncol. 2018 Jun;52(6):1815-1826. doi: 10.3892/ijo.2018.4346. Epub 2018 Mar 29. Upregulation of E-cadherin expression mediated by a novel dsRNA suppresses the growth and metastasis of bladder...
Zhang JH, Jiao LY, Li TJ, Zhu YY, Zhou JW, Tian J. J Cancer. 2017 Jun 3;8(9):1598-1608. doi: 10.7150/jca.18744. eCollection 2017. GSK-3β suppresses HCC cell dissociation in vitro by upregulating epithelial junction proteins and inhibiting Wnt/β-ca...