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SKU:MA1012
Clonality:Monoclonal
Clone Number:15F11
Application:IHC-F, ICC, WB
Price: $200.00
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Overview

Product Name Anti-Catenin Gamma (Plakoglobin) Antibody (Monoclonal, 15F11)
SKU/Catalog Number MA1012
Description Mouse IgG monoclonal antibody for Catenin gamma (Plakoglobin), junction plakoglobin ( JUP) detection. Tested with WB, IHC-F, ICC in Human;bovine. No cross reactivity with other proteins.
Cite This Product Anti-Catenin Gamma (Plakoglobin) Antibody (Monoclonal, 15F11) 15F11 (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1012)
Replacement Item This antibody may replace the following items: sc-83058|sc-98673|sc-271002 from Santa Cruz Biotechnology.
Host Mouse
Isotype Mouse IgG1
Validated Species Bovine, Human
Predicted Species Monkey

*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.

Application IHC-F, ICC, WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(F) and ICC.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen Recombinant chicken plakoglobin.
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Monoclonal
Clone Number 15F11
Form Lyophilized
Contents Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
*carrier free antibody available upon request.
Concentration Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Ascites
Isotype Mouse IgG1

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name Jup
Protein Name Junction plakoglobin
Molecular Weight 81801 MW
Protein Function Common junctional plaque protein. The membrane- associated plaques are architectural elements in an important strategic position to influence the arrangement and function of both the cytoskeleton and the cells within the tissue. The presence of plakoglobin in both the desmosomes and in the intermediate junctions suggests that it plays a central role in the structure and function of submembranous plaques. Acts as a substrate for VE-PTP and is required by it to stimulate VE- cadherin function in endothelial cells. Can replace beta-catenin in E-cadherin/catenin adhesion complexes which are proposed to couple cadherins to the actin cytoskeleton (By similarity). .
Sequence Similarities Belongs to the beta-catenin family.
Subcellular Localization Cell junction, adherens junction . Cell junction, desmosome . Cytoplasm, cytoskeleton . Membrane ; Peripheral membrane protein . Cytoplasmic in a soluble and membrane-associated form. .
Uniprot ID Q6P0K8
Alternative Names Junction plakoglobin;Jup;
Research Areas |signal transduction|cytoskeleton / ecm|cytoskeleton|microfilaments|actin etc|catenins|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Junction plakoglobin

Junction Plakoglobin(JUP) ,also knows as catenin gamma, is a major cytoplasmic protein that occurs in a soluble and a membrane-associated form and is the only known constituent common to the submembranous plaques of both kinds of adhering junctions, the desmosomes and the intermediate junctions. It is a component of the cadherin-catenin complex, which is predominantly localized where actin filaments anchor in adherens junctions of epithelial cells. The human plakoglobin gene localizes on chromosome 17q21. Gamma-catenin is regulated by the APC tumor suppressor and its oncogenic activity is distinct from that of beta-catenin.

Anti-Catenin Gamma (Plakoglobin) Antibody (Monoclonal, 15F11) Images

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Anti-Catenin Gamma (Plakoglobin) Antibody (Monoclonal, 15F11)
Anti-Catenin gamma(Plakoglobin) antibody (monoclonal), MA1012, Western blotting
Lane 1: MCF-7 Cell Lysate
Lane 2: HELA Cell Lysate
Lane 3: MM231 Cell Lysate
Lane 4: HT1080 Cell Lysate
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FAQs

Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: Can you provide monoclonal antibodies carrier free?
A: We cannot.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to gamma catenin antibody, plakoglobin antibody