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SKU:PB9046
Clonality:Polyclonal
Application:WB
Price: $240.00
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Overview

Product Name Anti-Cathepsin B Picoband™ Antibody
Description Rabbit IgG polyclonal antibody for Cathepsin B(CTSB) detection. Tested with WB in Human.
Cite This Product Anti-Cathepsin B Picoband™ Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9046)
Replacement Item This antibody may replace the following items: sc-136560|sc-136559|sc-136558|sc-136146|sc-136144|sc-136145 from Santa Cruz Biotechnology.
Host Rabbit
Isotype N/A
Validated Species Human
Application WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen E.coli-derived human Cathepsin B recombinant protein (Position: L80-D333). Human Cathepsin B shares 83% and 84% amino acid (aa) sequences identity with mouse and rat Cathepsin B, respectively.
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Polyclonal
Form Lyophilized
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
*carrier free antibody available upon request.
Concentration Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Immunogen affinity purified.
Isotype N/A

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name CTSB
Protein Name Cathepsin B
Molecular Weight 37822 MW
Protein Function Thiol protease which is believed to participate in intracellular degradation and turnover of proteins. Has also been implicated in tumor invasion and metastasis.
Sequence Similarities Belongs to the peptidase C1 family.
Subcellular Localization Lysosome. Melanosome. Secreted, extracellular space . Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
Uniprot ID P07858
Alternative Names Cathepsin B;3.4.22.1;APP secretase;APPS;Cathepsin B1;Cathepsin B light chain;Cathepsin B heavy chain;CTSB;CPSB;
Research Areas |neuroscience|cell adhesion proteins|membrane proteins| signal transduction|cytoskeleton / ecm|extracellular matrix|ecm enzymes|other enzymes| cancer|invasion/microenvironment|angiogenesis|cathespins|cell adhesion| kits/ lysates/ other|elisa kits|adhesion molecules elisa kits|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Cathepsin B

Cathepsin B is an enzymatic protein belonging to the peptidase or protease families. In humans, it is coded by the CTSB gene. And this gene is mapped to chromosome 8p22. The protein encoded by this gene is a lysosomal cysteine proteinase composed of a dimer of disulfide-linked heavy and light chains, both produced from a single protein precursor. It is a member of the peptidase C1 family. Cathepsin B was once suspected as a candidate protease participating in the conversion of beta-amyloid precursor protein into the amyloid plaques found in Alzheimer's disease patients. However, this function is now known to be mediated by BACE1 protease. It is now thought that cathepsin B can degrade beta-amyloid precursor protein into harmless fragments. Thus, it is conceivable cathepsin B may play a pivotal role in the natural defense against Alzheimer's disease. Overexpression of cathepsin B has been associated with esophageal adenocarcinoma and other tumors. At least five transcript variants encoding the same protein have been found for this gene. The standard product used in this kit is recombinant human Cathepsin B with the molecular mass of 37KDa.

Anti-Cathepsin B Picoband™ Antibody Images

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Anti-Cathepsin B Picoband™ Antibody
Anti-Cathepsin B Picoband antibody, PB9046-1.jpg
All lanes: Anti-Cathepsin B(PB9046) at 0.5ug/ml
WB: HEPG2 Whole Cell Lysate at 40ug
Predicted bind size: 38KD
Observed bind size: 38KD
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Publications

Zhang Zy, Mai Y, Yang H, Dong Py, Zheng Xl, Yang Gs. Mol Cell Biochem. 2014 Oct;395(1-2):53-64. Doi: 10.1007/S11010-014-2111-6. Epub 2014 May 31. Ctsb Promotes Porcine Preadipocytes Differentiation By Degrading Fibronectin And Attenuating The Wnt/...
Yang D, L?? X, Hong Y, Xi T, Zhang D. Biomaterials. 2014 Aug;35(24):6195-205. Doi: 10.1016/J.Biomaterials.2014.04.069. Epub 2014 May 10. The Molecular Mechanism For Effects Of Tin Coating On Niti Alloy On Endothelial Cell Function.

FAQs

Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
A: You can find the immunogen sequence under "Immunogen" and clonality in the product name.
Q: What is the expected band size? Why is it different than the observed band size?
A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands

3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.

4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
A: Check our protocols under the tech support tab.