Data & Images
|Product Name||Anti-Cathepsin D Antibody|
|Description||Mouse IgG monoclonal antibody for Cathepsin D, cathepsin D (CTSD) detection. Tested with WB, IHC-P in Human. No cross reactivity with other proteins.|
|Cite This Product||Anti-Cathepsin D Antibody CTD-19 (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1013)|
|Replacement Item||This antibody may replace the following items: sc-161659|sc-161658|sc-161658|sc-161659 from Santa Cruz Biotechnology.|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||Human liver cathepsin D.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
*carrier free antibody available upon request.
|Concentration||Add 1ml of PBS buffer will yield a concentration of 100ug/ml.|
|Storage||At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Cathepsin D|
|Molecular Weight||44681 MW|
|Protein Function||Acid protease active in intracellular protein breakdown.|
|Tissue Specificity||CTSD: Expressed in the aorta extrcellular space (atprotein level).|
|Sequence Similarities||Belongs to the peptidase A1 family.|
|Subcellular Localization||Lysosome. Melanosome . Secreted, extracellular space .|
|Alternative Names||Cathepsin D;220.127.116.11;Cathepsin D 12 kDa light chain;Cathepsin D 9 kDa light chain;Cathepsin D 34 kDa heavy chain;Cathepsin D 30 kDa heavy chain;Ctsd;|
|Research Areas|||neuroscience|cell adhesion proteins|membrane proteins| signal transduction|cytoskeleton / ecm|extracellular matrix|ecm enzymes|other enzymes| cancer|invasion/microenvironment| cell biology|proteolysis / ubiquitin|proteolytic enzymes|cysteine protease|cathepsins||
Background for Cathepsin D
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-Cathepsin D Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunohistochemistry(Paraffin-embedded Section), 1-2μg/ml, Human, By Heat
Western blot, 0.5-1μg/ml, Human
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-Cathepsin D Antibody Images
Click the images to enlarge.
IHC(P): Human Intestinal Cancer Tissue
IHC(P): Human Lung Cancer Tissue
IHC(P): Human Mammary Cancer Tissue