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SKU:MA1014
Pack Size:100μg/vial
Sample Size:30ug for $99, contact us for details
Clonality:Monoclonal
Clone Number:33/2
Application:IHC, WB
Price: $200.00
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Overview

Product Name Anti-Cathepsin L Antibody (Monoclonal, 33/2)
SKU/Catalog Number MA1014
Description Mouse IgG monoclonal antibody for Cathepsin L, cathepsin L1 (CTSL1) detection. Tested with WB, IHC-F in Human;mouse;rat. No cross reactivity with other proteins.
Cite This Product Anti-Cathepsin L Antibody (Monoclonal, 33/2) 33/2 (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1014)
Replacement Item This antibody may replace the following items: sc-31836|sc-7582|sc-30107|sc-74399|sc-31837|sc-7581|sc-74497|sc-31838|sc-31840 from Santa Cruz Biotechnology.
Host Mouse
Isotype Mouse IgG1
Validated Species Human, Mouse, Rat
Application IHC, WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(F).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen Procathepsin L isolated from the human lung cancer cell line EPLC 32M1.
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Monoclonal
Clone Number 33/2
Form Lyophilized
Contents Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
*carrier free antibody available upon request.
Concentration Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Ascites
Isotype Mouse IgG1

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name Ctsl
Protein Name Cathepsin L1
Molecular Weight 37660 MW
Protein Function Important for the overall degradation of proteins in lysosomes. Procathepsin L is required for maximal stimulation of steroidogenesis by TIMP1. .
Tissue Specificity Both mature cathepsin L1 and procathepsin L are found in the upper epidermis. The lower epidermis predominantly contains procathepsin L. In seminiferous tubules expression is greater at stages VI-VII than at stages IX-XII. .
Sequence Similarities Belongs to the peptidase C1 family.
Subcellular Localization Lysosome .
Uniprot ID P07154
Alternative Names Cathepsin L1;3.4.22.15;Cathepsin L;Cyclic protein 2;CP-2;Major excreted protein;MEP;Procathepsin L;Cathepsin L1 heavy chain;Cathepsin L1 light chain;Ctsl;Ctsl1;
Research Areas |neuroscience|cell adhesion proteins|membrane proteins| signal transduction|cytoskeleton / ecm|extracellular matrix|ecm enzymes|other enzymes|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Cathepsin L1

Cathepsin L is a lysosomal cysteine proteinase with a major role in intracellular protein catabolism. It also shows the most potent collagenolytic and elastinolytic activity in vitro of any of the cathepsins. Cathepsin L has been implicated in pathologic processes including myofibril necrosis in myopathies and in myocardial ischemia, and in the renal tubular response to proteinuria. Human liver cathepsin L consists of a heavy chain of about 25 kD and a light chain of about 5 kD. The gene is mapped to 9q21-q22. Cathepsin L is required for endothelial progenitor cell-induced neovascularization.
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FAQs

Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: Can you provide monoclonal antibodies carrier free?
A: We cannot.