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SKU:PB9165
Pack Size:100μg/vial
Sample Size:30ug for $99, contact us for details
Clonality:Polyclonal
Application:IHC, ICC, WB
Price: $240.00
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Overview

Product Name Anti-Caveolin-1 Picoband™ Antibody
SKU/Catalog Number PB9165
Description Rabbit IgG polyclonal antibody for Caveolin-1(CAV1) detection. Tested with WB, IHC-P, IHC-F, ICC in Human;Mouse.
Cite This Product Anti-Caveolin-1 Picoband™ Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9165)
Replacement Item This antibody may replace the following items: sc-101653|sc-14037|sc-14037-R|sc-28828|sc-373836|sc-373837|sc-53564|sc-70516|sc-7875|sc-894 from Santa Cruz Biotechnology.
Host Rabbit
Isotype N/A
Validated Species Human, Mouse
Application IHC, ICC, WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P) and IHC(F) and ICC.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen E.coli-derived human Caveolin-1 recombinant protein (Position: G4-I178). Human Caveolin-1 shares 95% and 94% amino acid (aa) sequences identity with mouse and rat Caveolin-1, respectively.
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Polyclonal
Form Lyophilized
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
*carrier free antibody available upon request.
Concentration Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Immunogen affinity purified.
Isotype N/A

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name CAV1
Protein Name Caveolin-1
Molecular Weight 20472 MW
Protein Function May act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity (By similarity). Involved in the costimulatory signal essential for T-cell receptor (TCR)- mediated T-cell activation. Its binding to DPP4 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3- dependent manner. Recruits CTNNB1 to caveolar membranes and may regulate CTNNB1-mediated signaling through the Wnt pathway. .
Tissue Specificity Expressed in muscle and lung, less so in liver, brain and kidney.
Sequence Similarities Belongs to the caveolin family.
Subcellular Localization Golgi apparatus membrane; Peripheral membrane protein. Cell membrane; Peripheral membrane protein. Membrane, caveola; Peripheral membrane protein. Membrane raft. Colocalized with DPP4 in membrane rafts. Potential hairpin- like structure in the membrane. Membrane protein of caveolae.
Uniprot ID Q03135
Alternative Names Caveolin-1;CAV1;CAV;
Research Areas |neuroscience|neurotransmission|calcium signaling|calcium channels|l-type| signal transduction|signaling pathway| cancer|cancer metabolism|metabolic signaling pathway|integration of energy metabolism| metabolism|pathways and processes|metabolic signaling pathways|energy transfer pathways|integration of energy|types of disease|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Caveolin-1

CAV1( Caveolin-1) is a protein that in humans is encoded by the CAV1 gene. The CAV1 gene is mapped to 7q31.2. The scaffolding protein encoded by this gene is the main component of the caveolae plasma membranes found in most cell types. The protein links integrin subunits to the tyrosine kinase FYN, an initiating step in coupling integrins to the Ras-ERK pathway and promoting cell cycle progression. The gene is a tumor suppressor gene candidate and a negative regulator of the Ras-p42/44 MAP kinase cascade. CAV1 and CAV2 are located next to each other on chromosome 7 and express colocalizing proteins that form a stable hetero-oligomeric complex. By using alternative initiation codons in the same reading frame, two isoforms(alpha and beta) are encoded by a single transcript from this gene.

Anti-Caveolin-1 Picoband™ Antibody Images

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Anti-Caveolin-1 Picoband™ Antibody
Anti- Caveolin-1 picoband antibody, PB9165, Western blotting
All lanes: Anti Caveolin-1 (PB9165) at 0.5ug/ml
WB: Recombinant Human Caveolin-1 Protein 0.5ng
Predicted bind size: 40KD
Observed bind size: 40KD
Anti-Caveolin-1 Picoband™ Antibody
Anti- Caveolin-1 picoband antibody, PB9165, Western blotting
All lanes: Anti Caveolin-1 (PB9165) at 0.5ug/ml
Lane 1: Hela Whole Cell Lysate at 40ug
Lane 2: HT1080 Whole Cell Lysate at 40ug
Lane 3: Human Placenta Tissue Lysate at 50ug
Lane 4: A431 Whole Cell Lysate at 40ug
Predicted bind size: 21KD
Observed bind size: 21KD
Anti-Caveolin-1 Picoband™ Antibody
Anti- Caveolin-1 picoband antibody, PB9165, IHC(P)
IHC(P): Human Placenta Tissue
Anti-Caveolin-1 Picoband™ Antibody
Anti- Caveolin-1 picoband antibody, PB9165, IHC(P)
IHC(P): Human Lung Cancer Tissue
Anti-Caveolin-1 Picoband™ Antibody
Anti- Caveolin-1 picoband antibody, PB9165, ICC
ICC: A549 Cell
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Publications

Wang Nn, Zhao Lj, Wu Ln, He Mf, Qu Jw, Zhao Yb, Zhao Wz, Li Js, Wang Jh. Asian Pac J Cancer Prev. 2013;14(9):4983-8. Mechanistic Analysis Of Taxol-Induced Multidrug Resistance In An Ovarian Cancer Cell Line.

FAQs

Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
A: You can find the immunogen sequence under "Immunogen" and clonality in the product name.
Q: What is the expected band size? Why is it different than the observed band size?
A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands

3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.

4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
A: Check our protocols under the tech support tab.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to caveolin-1 antibody, cav 1 antibody, cav1 antibody