|Application:||IF, IHC-P, WB|
Data & Images
|Product Name||Anti-Caveolin-2 Picoband™ Antibody|
|Description||Rabbit IgG polyclonal antibody for Caveolin-2(CAV2) detection. Tested with WB, IHC-P, IHC-F in Human;Mouse.|
|Cite This Product||Anti-Caveolin-2 Picoband™ Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9166)|
|Replacement Item||This antibody may replace the following items: sc-1858|sc-27998-R|sc-30754|sc-30755|sc-7942 from Santa Cruz Biotechnology.|
|Validated Species||Human, Mouse|
|Application||IF, IHC-P, WB
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P) and IHC(F).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||E.coli-derived human Caveolin-2 recombinant protein (Position: M1-D162). Human Caveolin-2 shares 90% and 89% amino acid (aa) sequences identity with mouse and rat Caveolin-2, respectively.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
*carrier free antibody available upon request.
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Molecular Weight||18291 MW|
|Protein Function||May act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity. Acts as an accessory protein in conjunction with CAV1 in targeting to lipid rafts and driving caveolae formation. The Ser-36 phosphorylated form has a role in modulating mitosis in endothelial cells. Positive regulator of cellular mitogenesis of the MAPK signaling pathway. Required for the insulin-stimulated nuclear translocation and activation of MAPK1 and STAT3, and the subsequent regulation of cell cycle progression (By similarity). .|
|Tissue Specificity||Expressed in endothelial cells, smooth muscle cells, skeletal myoblasts and fibroblasts. .|
|Sequence Similarities||Belongs to the caveolin family.|
|Subcellular Localization||Nucleus. Cytoplasm. Golgi apparatus membrane; Peripheral membrane protein. Cell membrane; Peripheral membrane protein. Membrane, caveola; Peripheral membrane protein. Potential hairpin-like structure in the membrane. Membrane protein of caveolae. Tyr-19-phosphorylated form is enriched at sites of cell-cell contact and is translocated to the nucleus in complex with MAPK1 in response to insulin (By similarity). Tyr-27- phosphorylated form is located both in the cytoplasm and plasma membrane. CAV1-mediated Ser-23-phosphorylated form locates to the plasma membrane. Ser-36-phosphorylated form resides in intracellular compartments. .|
|Research Areas|||neuroscience|neurotransmission|calcium signaling|calcium channels|p / q / r -type| signal transduction|signaling pathway| cancer|cancer metabolism|metabolic signaling pathway|integration of energy metabolism| metabolism|pathways and processes|metabolic signaling pathways|energy transfer pathways|integration of energy|types of disease||
Background for Caveolin-2
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-Caveolin-2 Picoband™ Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunohistochemistry(Frozen Section), 0.5-1μg/ml, Human, Mouse, -|
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-Caveolin-2 Picoband™ Antibody Images
Click the images to enlarge.
All lanes: Anti Caveolin-2 (PB9166) at 0.5ug/ml
WB: Recombinant Human Caveolin-2 Protein 0.5ng
Predicted bind size: 40KD
Observed bind size: 40KD
All lanes: Anti Caveolin-2 (PB9166) at 0.5ug/ml
Lane 1: Hela Whole Cell Lysate at 40ug
Lane 2: HT1080 Whole Cell Lysate at 40ug
Lane 3: Human Placenta Tissue Lysate at 50ug
Lane 4: A431 Whole Cell Lysate at 40ug
Predicted bind size: 17KD
Observed bind size: 17KD
IHC(P): Human Intestinal Cancer Tissue
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,