Data & Images
|Product Name||Anti-CBS Antibody|
|Description||Rabbit IgG polyclonal antibody for Cystathionine beta-synthase(CBS) detection. Tested with WB, IHC-P in Human;Rat.|
|Cite This Product||Anti-CBS Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # RP1041)|
|Replacement Item||This antibody may replace the following items: sc-46832|sc-46831|sc-46830|sc-67154|sc-271886|sc-133154|sc-133208 from Santa Cruz Biotechnology.|
|Validated Species||Human, Rat|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||E.coli-derived human CBS recombinant protein (Position: A331-K551). Human CBS shares 83% amino acid (aa) sequence identity with both mouse and rat CBS.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
*carrier free antibody available upon request.
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Cystathionine beta-synthase|
|Molecular Weight||60587 MW|
|Protein Function||Only known pyridoxal phosphate-dependent enzyme that contains heme. Important regulator of hydrogen sulfide, especially in the brain, utilizing cysteine instead of serine to catalyze the formation of hydrogen sulfide. Hydrogen sulfide is a gastratransmitter with signaling and cytoprotective effects such as acting as a neuromodulator in the brain to protect neurons against hypoxic injury (By similarity). .|
|Tissue Specificity||In the adult strongly expressed in liver and pancreas, some expression in heart and brain, weak expression in lung and kidney. In the fetus, expressed in brain, liver and kidney.|
|Sequence Similarities||Belongs to the cysteine synthase/cystathionine beta- synthase family.|
|Subcellular Localization||Cytoplasm . Nucleus .|
|Alternative Names||Cystathionine beta-synthase;126.96.36.199;Beta-thionase;Serine sulfhydrase;CBS;|
|Research Areas|||signal transduction|metabolism|amino acids| cancer|cancer metabolism|cellular metabolic process| metabolism|pathways and processes|metabolic signaling pathways|amino acid metabolism|types of disease||
Background for Cystathionine beta-synthase
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-CBS Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat|
Western blot, 0.1-0.5μg/ml, Human, Rat
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-CBS Antibody Images
Click the images to enlarge.
All lanes: Anti CBS (RP1041) at 0.5ug/ml
WB: Recombinant human CBS Protein 0.5ng
Predicted bind size: 42KD
Observed bind size: 42KD
All lanes: Anti CBS (RP1041) at 0.5ug/ml
Lane 1: Rat Liver Tissue Lysate at 50ug
Lane 2: Rat Brain Tissue Lysate at 50ug
Lane 3: Hela Whole Cell Lysate at 40ug
Lane 4: PANC Whole Cell Lysate at 40ug
Lane 5: Hepg2 Whole Cell Lysate at 40ug
Predicted bind size: 60KD
Observed bind size: 60KD
IHC(P): Human Liver Cancer Tissue
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,