Data & Images
|Product Name||Anti-CCL18/PARC Antibody|
|Description||Rabbit IgG polyclonal antibody for C-C motif chemokine 18(CCL18) detection. Tested with WB, ELISA in Human.|
|Cite This Product||Anti-CCL18/PARC Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9023)|
|Replacement Item||This antibody may replace the following items: sc-134412|sc-33259|sc-33260|sc-374438|sc-374439|sc-53748|sc-53810|sc-67019|sc-9781 from Santa Cruz Biotechnology.|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||E.coli-derived human CCL18 recombinant protein (Position: A21-A89).|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
*carrier free antibody available upon request.
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||C-C motif chemokine 18|
|Molecular Weight||9849 MW|
|Protein Function||Chemotactic factor that attracts lymphocytes but not monocytes or granulocytes. May be involved in B-cell migration into B-cell follicles in lymph nodes. Attracts naive T-lymphocytes toward dendritic cells and activated macrophages in lymph nodes, has chemotactic activity for naive T-cells, CD4+ and CD8+ T-cells and thus may play a role in both humoral and cell-mediated immunity responses. .|
|Tissue Specificity||Expressed at high levels in lung, lymph nodes, placenta, bone marrow, dendritic cells present in germinal centers and T-cell areas of secondary lymphoid organs and macrophages derived from peripheral blood monocytes. Not expressed by peripheral blood monocytes and a monocyte-to-macrophage differentiation is a prerequisite for expression. Expressed in synovial fluids from patients with rheumatoid and septic arthritis and in ovarian carcinoma ascitic fluid. .|
|Sequence Similarities||Belongs to the intercrine beta (chemokine CC) family.|
|Alternative Names||C-C motif chemokine 18;Alternative macrophage activation-associated CC chemokine 1;AMAC-1;CC chemokine PARC;Dendritic cell chemokine 1;DC-CK1;Macrophage inflammatory protein 4;MIP-4;Pulmonary and activation-regulated chemokine;Small-inducible cytokine A18;CCL18(1-68);CCL18(3-69);CCL18(4-69);CCL18;AMAC1, DCCK1, MIP4, PARC, SCYA18;|
|Research Areas|||immunology|innate immunity|chemokines|beta chemokines (cc)| cancer|tumor biomarkers|oncoproteins| kits/ lysates/ other|elisa kits|oncoprotein elisa kits|cytokines and cytokine receptors elisa kits||
Background for C-C motif chemokine 18
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-CCL18/PARC Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Western blot, 0.1-0.5μg/ml, Human, -|
ELISA , 0.1-0.5μg/ml, Human
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-CCL18/PARC Antibody Images
Click the images to enlarge.
All lanes: Anti-CCL18(PB9023) at 0.5ug/ml
WB: Recombinant Human CCL18 Protein 0.5ng
Predicted bind size: 19KD
Observed bind size: 19KD
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,