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SKU:PB9058
Pack Size:100μg/vial
Sample Size:30ug for $99, contact us for details
Clonality:Polyclonal
Application:IHC, WB
Price: $240.00
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Overview

Product Name Anti-CD10/Neprilysin Antibody
SKU/Catalog Number PB9058
Description Rabbit IgG polyclonal antibody for Neprilysin(MME) detection. Tested with WB, IHC-P in Human;Mouse;Rat.
Cite This Product Anti-CD10/Neprilysin Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9058)
Replacement Item This antibody may replace the following items: sc-101449|sc-102424|sc-102426|sc-12361|sc-133151|sc-19993|sc-30072|sc-31805|sc-31807|sc-31809|sc-46656|sc-52455|sc-65990|sc-7632|sc-80544|sc-9149 from Santa Cruz Biotechnology.
Host Rabbit
Isotype N/A
Validated Species Human, Mouse, Rat
Application IHC, WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen E.coli-derived human CD10 recombinant protein (Position: Y52-W750). Human CD10 shares 94% amino acid (aa) sequences identity with both mouse and rat CD10.
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Polyclonal
Form Lyophilized
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
*carrier free antibody available upon request.
Concentration Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Immunogen affinity purified.
Isotype N/A

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name MME
Protein Name Neprilysin
Molecular Weight 85514 MW
Protein Function Thermolysin-like specificity, but is almost confined on acting on polypeptides of up to 30 amino acids (PubMed:15283675, PubMed:8168535). Biologically important in the destruction of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond (PubMed:17101991). Able to cleave angiotensin-1, angiotensin-2 and angiotensin 1-9 (PubMed:15283675). Involved in the degradation of atrial natriuretic factor (ANF) (PubMed:2531377, PubMed:2972276). Displays UV-inducible elastase activity toward skin preelastic and elastic fibers (PubMed:20876573). .
Sequence Similarities Belongs to the peptidase M13 family.
Subcellular Localization Cell membrane; Single-pass type II membrane protein.
Uniprot ID P08473
Alternative Names Neprilysin;3.4.24.11 ;Atriopeptidase;Common acute lymphocytic leukemia antigen;CALLA;Enkephalinase;Neutral endopeptidase 24.11;NEP;Neutral endopeptidase;Skin fibroblast elastase;SFE;CD10;MME;EPN;
Research Areas MME|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Neprilysin

CD10, also known as membrane metallo-endopeptidase, neutral endopeptidase(NEP), Neprilysin, or common acute lymphoblastic leukemia antigen(CALLA), is a zinc-dependent metalloprotease enzyme that degrades a number of small secreted peptides, most notably theamyloid beta peptide whose abnormal misfolding and aggregation in neural tissue has been implicated as a cause of Alzheimer's disease. This gene is localized to human chromosome 3 by study of somatic cell hybrids and regionalized the location to 3q21-q27 by in situ hybridization. By cDNA transfection analysis, CD10 is confirmed as a functional neutral endopeptidase of the type that has previously been called enkephalinase. CD10 has also been called atriopeptidase. Atriopeptidase specifically degrades atrial natriuretic factor. A specific enzyme inhibitor was developed and reported that it had effects similar to those of low-dose ANF infusion. These effects include diuresis, natriuresis, vasodilatation, and suppression of the renin-angiotensin-aldosterone system.

Anti-CD10/Neprilysin Antibody Images

Click the images to enlarge.

Anti-CD10/Neprilysin Antibody
Anti-CD10 Picoband antibody, PB9058-1.JPG
IHC(P): Mouse Kidney Tissue
Anti-CD10/Neprilysin Antibody
Anti-CD10 Picoband antibody, PB9058-2.JPG
IHC(P): Human B lymphocyte Cancer Tissue
Anti-CD10/Neprilysin Antibody
Anti-CD10 Picoband antibody, PB9058-3.JPG
IHC(P): Mouse Spleen Tissue
Anti-CD10/Neprilysin Antibody
Anti-CD10 Picoband antibody, PB9058-4.JPG
IHC(P): Rat Spleen Tissue
Anti-CD10/Neprilysin Antibody
Anti-CD10 Picoband antibody, PB9058-5.jpg
All lanes: Anti-CD10(PB9058) at 0.5ug/ml
Lane 1: Rat Kidney Tissue Lysate at 40ug
Lane 2: Rat Brain Tissue Lysate at 40ug
Lane 3: Rat Liver Tissue Lysate at 40ug
Lane 4: Human Placenta Tissue Lysate at 40ug
Lane 5: HELA Whole Cell Lysate at 40ug
Lane 6: JURKAT Whole Cell Lysate at 40ug
Lane 7: RAJI Whole Cell Lysate at 40ug
Lane 8: 293T Whole Cell Lysate at 40ug
Predicted bind size: 86KD
Observed bind size: 100KD

Anti-CD-10 Antibody Reviews

Source: Biocompare.com

"Cheap, prompt shipping"

--Anthoni Goodman CELL, DEVELOPMENTAL, AND INTEGRATIVE BIOLOGY, UNIVERSITY OF ALABAMA AT BIRMINGHAM, GRADUATE STUDENT/RESEARCH TRAINEE

Applications Western Blot
Sample Rat PFC
Detection Protein Simple, Simple Western size-based assay

"We selected this product as a polyclonal ab for our Tg Rat colony. We are interested in adding enzymatic catabolism/clearance of amyloid beta isoforms into our body of research. We purchased this product because it was cheap and we are looking at some 'proof-of-concept' and didn't need remarkable specificity. We still have much to investigate."

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Publications

Meng Hx, Li Hn, Geng Js, Ohe R, Yu Xy, E Xq, Ye F, Yang Sr, Kato T, Zhang L, Ishida A, Ohta N, Jin Xm, Kakehata S, Geng Js, Yamakawa M. Transl Res. 2015 Sep;166(3):281-91. Doi: 10.1016/J.Trsl.2015.04.004. Epub 2015 Apr 17. Decreased Expression Of ...

FAQs

Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
A: You can find the immunogen sequence under "Immunogen" and clonality in the product name.
Q: What is the expected band size? Why is it different than the observed band size?
A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands

3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.

4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
A: Check our protocols under the tech support tab.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to cd10 antibody, gp100 antibody, neprilysin antibody