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SKU:PA1395
Pack Size:100μg/vial
Sample Size:30ug for $99, contact us for details
Clonality:Polyclonal
Application:IHC-P, WB
Price: $200.00
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Overview

Product Name Anti-CD105 Antibody
SKU/Catalog Number PA1395
Description Rabbit IgG polyclonal antibody for Endoglin(ENG) detection. Tested with WB, IHC-P in Human.
Cite This Product Anti-CD105 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1395)
Replacement Item This antibody may replace the following items: sc-18838|sc-19790|sc-20072|sc-20632|sc-21787|sc-23838|sc-376381|sc-53999|sc-57099|sc-6199 from Santa Cruz Biotechnology.
Host Rabbit
Isotype N/A
Validated Species Human
Application IHC-P, WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen A synthetic peptide corresponding to a sequence at the N-terminus of human CD105(26-44aa ETVHCDLQPVGPERGEVTY).
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Polyclonal
Form Lyophilized
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
Concentration Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Immunogen affinity purified.
Isotype N/A

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name ENG
Protein Name Endoglin
Molecular Weight 70578 MW
Protein Function Major glycoprotein of vascular endothelium. Involved in the regulation of angiogenesis. May play a critical role in the binding of endothelial cells to integrins and/or other RGD receptors. Acts as TGF-beta coreceptor and is involved in the TGF- beta/BMP signaling cascade. Required for GDF2/BMP9 signaling through SMAD1 in endothelial cells and modulates TGF-beta1 signaling through SMAD3. .
Tissue Specificity Endoglin is restricted to endothelial cells in all tissues except bone marrow.
Subcellular Localization Membrane; Single-pass type I membrane protein.
Uniprot ID P17813
Alternative Names Endoglin;CD105;ENG;END;
Research Areas |neuroscience|neurology process|neurogenesis| epigenetics and nuclear signaling|transcription|other factors|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Endoglin

Endoglin(Osler-Rendu-Weber syndrome 1), CD105, is a type I membrane glycoprotein located on cell surfaces and is a part of the TGF beta receptor complex. Its gene is mapped to human chromosome 8. The protein consists of a homodimer of 180 kDA with disulfide links. It has been found on endothelial cells, activated macrophages, fibroblasts and smooth muscle cells. Endoglin has a role in the development of the cardiovascular system and in vascular remodeling and has been found to be elevated in pregnant women who subsequently develop preeclampsia.

Anti-CD105 Antibody Images

Click the images to enlarge.

Anti-CD105 Antibody
Anti-CD105 antibody, PA1395, Western blotting
Lane 1: Recombinant Human CD105 Protein 10ng
Lane 2: Recombinant Human CD105 Protein 5ng
Lane 3: Recombinant Human CD105 Protein 2.5ng
Anti-CD105 Antibody
Anti-CD105 antibody, PA1395, IHC(P)
IHC(P): Human Mammary Cancer Tissue
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Publications

Su L, Ji J, Bian J, Fu Y, Ge Y, Yuan Z. Int Immunopharmacol. 2012 Dec;14(4):606-12. Doi: 10.1016/J.Intimp.2012.09.010. Epub 2012 Sep 30. Tacrolimus (Fk506) Prevents Early Retinal Neovascularization In Streptozotocin-Induced Diabetic Mice.
Ren Sx, Ren Zj, Zhao My, Wang Xb, Zuo Sg, Yu F. Acta Pharmacol Sin. 2009 May;30(5):637-45. Doi: 10.1038/Aps.2009.44. Antitumor Activity Of Endogenous Mflt4 Displayed On A T4 Phage Nanoparticle Surface.
Ren S, Fengyu, Zuo S, Zhao M, Wang X, Wang X, Chen Y, Wu Z, Ren Z. Vaccine. 2011 Aug 5;29(34):5802-11. Doi: 10.1016/J.Vaccine.2011.03.051. Epub 2011 Apr 9. Inhibition Of Tumor Angiogenesis In Lung Cancer By T4 Phage Surface Displaying Mvegfr2 Vacc...
Li J, Zeng G, Qi Y, Tang X, Zhang J, Wu Z, Liang J, Shi L, Liu H, Zhang P. Plos One. 2015 Apr 7;10(4):E0123264. Doi: 10.1371/Journal.Pone.0123264. Ecollection 2015. Xenotransplantation Of Human Adipose-Derived Stem Cells In Zebrafish Embryos.
Dai J, Li Y, Zhou H, Chen J, Chen M, Xiao Z. Int J Biol Sci. 2013 Nov 21;9(10):1089-98. Doi: 10.7150/Ijbs.7367. Ecollection 2013. Genistein Promotion Of Osteogenic Differentiation Through Bmp2/Smad5/Runx2 Signaling.

FAQs

Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
A: You can find the immunogen sequence under "Immunogen" and clonality in the product name.
Q: What is the expected band size? Why is it different than the observed band size?
A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands

3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.

4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
A: Check our protocols under the tech support tab.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to cd105 antibody, endoglin antibody