Product Info Summary
SKU: | M01199 |
---|---|
Size: | 100ug/vial |
Reactive Species: | Human |
Host: | Mouse |
Application: | IHC |
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Product info
Product Name
Anti-CD106 / VCAM1 (Activated Endothelial Cell Marker) Monoclonal Antibody
View all VCAM-1/CD106 Antibodies
SKU/Catalog Number
M01199
Size
100ug/vial
Form
Liquid
Description
Boster Bio Anti-CD106 / VCAM1 (Activated Endothelial Cell Marker) Monoclonal Antibody (Catalog # M01199). Tested in IHC applications. This antibody reacts with Human.
Storage & Handling
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Cite This Product
Anti-CD106 / VCAM1 (Activated Endothelial Cell Marker) Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01199)
Host
Mouse
Contents
Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Clonality
Monoclonal
Clone Number
Clone: VCAM1/843
Isotype
IgG1, kappa
Immunogen
Recombinant human VCAM1 protein
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Does not cross-react with primate, avian or amphibian GR.
Reactive Species
M01199 is reactive to VCAM1 in Human
Applications
M01199 is guaranteed for IHC Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
81.276kDa
Background of VCAM-1/CD106
Recognizes a protein of 110kDa, identified as CD106 (also known as vascular cell adhesion molecule-1 (VCAM-1) and INCAM-100). CD106 is a member of the Ig superfamily of adhesion molecules and is expressed at high levels on cytokine stimulated vascular endothelial cells, and at minimal levels on un-stimulated endothelial cells. It is also present on follicular and inter-follicular dendritic cells of lymph nodes, myoblasts, and some macrophages. CD106 serves as a ligand for leukocyte integrin (VLA-4 or CD49d/CD29) and mediates cell adhesion of leukocytes to activated endothelium. It plays a role in various immunological and inflammatory responses.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Reconstitute with distilled water.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
Validation Images & Assay Conditions
Click image to see more details
Formalin-fixed, paraffin-embedded human Tonsil stained with Anti-CD106 Monoclonal Antibody (VCAM1/843).
Protein Target Info & Infographic
Gene/Protein Information For VCAM1 (Source: Uniprot.org, NCBI)
Gene Name
VCAM1
Full Name
Vascular cell adhesion protein 1
Weight
81.276kDa
Alternative Names
CD106 antigen; CD106; DKFZp779G2333; INCAM-100; L1CAM; MGC99561; vascular cell adhesion molecule 1; vascular cell adhesion protein 1; V-CAM 1; VCAM1; VCAM-1 VCAM1 CD106, INCAM-100 vascular cell adhesion molecule 1 vascular cell adhesion protein 1|CD106 antigen
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on VCAM1, check out the VCAM1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for VCAM1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CD106 / VCAM1 (Activated Endothelial Cell Marker) Monoclonal Antibody (M01199)
Hello CJ!
M01199 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Retinoic Acid Promotes Interleukin-4 Plasmid-Dimethylsulfoxide Topical Transdermal Delivery for Treatment of Psoriasis
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Customer Reviews
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Customer Q&As
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3 Customer Q&As for Anti-CD106 / VCAM1 (Activated Endothelial Cell Marker) Monoclonal Antibody
Question
Can you help my question with product M01199, anti-CD106 / VCAM1 (Activated Endothelial Cell Marker) Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-06-26
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M01199 anti-CD106 / VCAM1 (Activated Endothelial Cell Marker) Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-06-26
Question
I was wanting to use your anti-CD106 / VCAM1 (Activated Endothelial Cell Marker) Monoclonal antibody for IHC for human umbilical vein endothelial cell on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human umbilical vein endothelial cell identification?
G. Lewis
Verified customer
Asked: 2019-03-15
Answer
You can see on the product datasheet, M01199 anti-CD106 / VCAM1 (Activated Endothelial Cell Marker) Monoclonal antibody has been validated for IHC on human tissues. We have an innovator award program that if you test this antibody and show it works in human umbilical vein endothelial cell in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-03-15
Question
Do you have a BSA free version of anti-CD106 / VCAM1 (Activated Endothelial Cell Marker) Monoclonal antibody M01199 available?
Verified Customer
Verified customer
Asked: 2018-03-16
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-CD106 / VCAM1 (Activated Endothelial Cell Marker) Monoclonal antibody M01199 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-03-16