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SKU:PB9140
Pack Size:100μg/vial
Sample Size:30ug for $99, contact us for details
Clonality:Polyclonal
Application:IHC-P, WB
Price: $240.00
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Overview

Product Name Anti-CD11b Picoband™ Antibody
SKU/Catalog Number PB9140
Description Rabbit IgG polyclonal antibody for Integrin alpha-M(ITGAM) detection. Tested with WB, IHC-P in Human;Mouse;Rat.
Cite This Product Anti-CD11b Picoband™ Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9140)
Replacement Item This antibody may replace the following items: sc-1186|sc-20050|sc-23934|sc-23937|sc-28664|sc-51657|sc-59744|sc-65429|sc-6612|sc-71444|sc-80542|sc-81721|sc-81723 from Santa Cruz Biotechnology.
Host Rabbit
Isotype N/A
Validated Species Human, Mouse, Rat
Application IHC-P, WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen E.coli-derived human CD11b recombinant protein (Position: F17-T382). Human CD11b shares 79% and 61% amino acid (aa) sequences identity with mouse and rat CD11b, respectively.
Cross Reactivity No cross reactivity with other proteins.
Pack Size 100μg/vial

Properties

Clonality Polyclonal
Form Lyophilized
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
*carrier free antibody available upon request.
Concentration Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Immunogen affinity purified.
Isotype N/A

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name ITGAM
Protein Name Integrin alpha-M
Molecular Weight 127179 MW
Protein Function Integrin alpha-M/beta-2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin alpha-M/beta-2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain.
Tissue Specificity Predominantly expressed in monocytes and granulocytes.
Sequence Similarities Belongs to the integrin alpha chain family.
Subcellular Localization Membrane; Single-pass type I membrane protein.
Uniprot ID P11215
Alternative Names Integrin alpha-M;CD11 antigen-like family member B;CR-3 alpha chain;Cell surface glycoprotein MAC-1 subunit alpha;Leukocyte adhesion receptor MO1;Neutrophil adherence receptor;CD11b;ITGAM;CD11B, CR3A;
Research Areas ITGAM|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Integrin alpha-M

Integrin alpha M (ITGAM) is one protein subunit that forms the heterodimeric integrin alpha-M beta-2 (αMβ2) molecule, also known as macrophage-1 antigen (Mac-1) or complement receptor 3 (CR3). It is mapped to 16p11.2. ITGAM has a role in vascular repair after mechanical arterial injury. It is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. What's more, ITGAM probably recognizes the R-G-D peptide in C3b, and it is also a receptor for fibrinogen, factor X and ICAM1.

Anti-CD11b Picoband™ Antibody Images

Click the images to enlarge.

Anti-CD11b Picoband™ Antibody
Anti-CD11b Picoband antibody , PB9140-1.jpg
All lanes: Anti CD11B (PB9140) at 0.5ug/ml
WB: Recombinant Human CD11B Protein 0.5ng
Predicted bind size: 45KD
Observed bind size: 45KD
Anti-CD11b Picoband™ Antibody
Anti-CD11b Picoband antibody , PB9140-2.JPG
IHC(P): Human Tonsil Tissue
Anti-CD11b Picoband™ Antibody
Anti-CD11b Picoband antibody , PB9140-3.JPG
IHC(P): Mouse Spleen Tissue
Anti-CD11b Picoband™ Antibody
Anti-CD11b Picoband antibody , PB9140-4.JPG
IHC(P): Rat Spleen Tissue
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Publications

Ren B, Zhang Yx, Zhou Hx, Sun Fw, Zhang Zf, Wei Z, Zhang Cy, Si Dw. J Neurol Sci. 2015 Jan 15;348(1-2):142-52. Doi: 10.1016/J.Jns.2014.11.026. Epub 2014 Nov 28. Tanshinone Iia Prevents The Loss Of Nigrostriatal Dopaminergic Neurons By Inhibiting N...
Zhao G, Wang S, Wang Z, Sun A, Yang X, Qiu Z, Wu C, Zhang W, Li H, Zhang Y, Zhao J, Zou Y, Ge J. Int J Cardiol. 2013 Sep 30;168(2):853-62. Doi: 10.1016/J.Ijcard.2012.10.022. Epub 2012 Nov 13. Cxcr6 Deficiency Ameliorated Myocardial Ischemia/Reperf...
Mu L, Jing C, Guo Z. Iran J Basic Med Sci. 2014 Nov;17(11):874-8. Expressions Of Cd11A, Cd11B, And Cd11C Integrin Proteins In Rats With Myocardial Hypertrophy.

FAQs

Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
A: You can find the immunogen sequence under "Immunogen" and clonality in the product name.
Q: What is the expected band size? Why is it different than the observed band size?
A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands

3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.

4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
A: Check our protocols under the tech support tab.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to cd11b antibody, mac 1 antibody, mac-1 antibody