|Sample Size:||30ug for $99, contact us for details|
Data & Images
|Product Name||Anti-CD14 Antibody|
|Description||Rabbit IgG polyclonal antibody for Monocyte differentiation antigen CD14(CD14) detection. Tested with WB in Mouse.|
|Cite This Product||Anti-CD14 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1443)|
|Replacement Item||This antibody may replace the following items: sc-5749|sc-6999|sc-1182|sc-19588|sc-6998|sc-9150|sc-7328|sc-52457|sc-58951 from Santa Cruz Biotechnology.|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||A synthetic peptide corresponding to a sequence in the middle region of mouse CD14(152-165aa TRDAWLAELQQWLK).|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Monocyte differentiation antigen CD14|
|Molecular Weight||39204 MW|
|Protein Function||In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the MD-2/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules (By similarity). .|
|Subcellular Localization||Cell membrane; Lipid-anchor, GPI-anchor.|
|Alternative Names||Monocyte differentiation antigen CD14;Myeloid cell-specific leucine-rich glycoprotein;CD14;Cd14;|
|Research Areas|||immunology|cell type markers|myeloid cells| stem cells|hematopoietic progenitors|hematopoietic stem cells|human lineage negative|mesenchymal stem cells|negative markers|myeloid|monocytic lineage|neutrophil lineage| immunology|innate immunity|tlr signaling|endothelial progenitors|endothelial markers| kits/ lysates/ other|elisa kits|cd markers elisa kits||
Background for Monocyte differentiation antigen CD14
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-CD14 Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Western blot, 0.1-0.5μg/ml, Mouse|
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-CD14 Antibody Images
Click the images to enlarge.
Lane 1: Mouse Thymus Tissue Lysate
Lane 2: Mouse Spleen Tissue Lysate
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,