Data & Images
|Product Name||Anti-Emmprin Antibody|
|Description||Rabbit IgG polyclonal antibody for Basigin(BSG) detection. Tested with WB in Human.|
|Cite This Product||Anti-Emmprin Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1526)|
|Replacement Item||This antibody may replace the following items: sc-30932|sc-30931|sc-166474|sc-17222|sc-376369|sc-74551|sc-376370|sc-136230|sc-20132 from Santa Cruz Biotechnology.|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||A synthetic peptide corresponding to a sequence in the middle region of human CD147(186-203aa QKTEFKVDSDDQWGEYSC).|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Molecular Weight||42200 MW|
|Protein Function||Plays an important role in targeting the monocarboxylate transporters SLC16A1, SLC16A3 and SLC16A8 to the plasma membrane. Plays pivotal roles in spermatogenesis, embryo implantation, neural network formation and tumor progression. Stimulates adjacent fibroblasts to produce matrix metalloproteinases (MMPS). Seems to be a receptor for oligomannosidic glycans. In vitro, promotes outgrowth of astrocytic processes. .|
|Tissue Specificity||Present only in vascular endothelium in non- neoplastic regions of the brain, whereas it is present in tumor cells but not in proliferating blood vessels in malignant gliomas.|
|Sequence Similarities||Contains 1 Ig-like C2-type (immunoglobulin-like) domain.|
|Subcellular Localization||Cell membrane ; Single-pass type I membrane protein . Melanosome . Colocalizes with SLC16A1 and SLC16A8. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. .|
|Alternative Names||Basigin;5F7;Collagenase stimulatory factor;Extracellular matrix metalloproteinase inducer;EMMPRIN;Leukocyte activation antigen M6;OK blood group antigen;Tumor cell-derived collagenase stimulatory factor;TCSF;CD147;BSG;UNQ6505/PRO21383;|
|Research Areas|||signal transduction|cytoskeleton / ecm|extracellular matrix|ecm enzymes|mmp| neuroscience|sensory system|visual system| cancer|invasion/microenvironment|mmps|cancer metabolism|metabolic signaling pathway|metabolism of carbohydrates| kits/ lysates/ other|elisa kits|cd markers elisa kits| metabolism|pathways and processes|metabolic signaling pathways|carbohydrate metabolism|types of disease||
Background for Basigin
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-Emmprin Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Western blot, 0.1-0.5μg/ml, Human|
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-Emmprin Antibody Images
Click the images to enlarge.
WB: Human Fibroma Cell Lysate
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,