Data & Images
|Product Name||Anti-CD163 Antibody|
|Description||Rabbit IgG polyclonal antibody for Scavenger receptor cysteine-rich type 1 protein M130(CD163) detection. Tested with WB in Human.|
|Cite This Product||Anti-CD163 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1874)|
|Replacement Item||This antibody may replace the following items: sc-33560|sc-58965|sc-33715|sc-18796|sc-33559|sc-20066|sc-18794 from Santa Cruz Biotechnology.|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human CD163(1092-1109aa HQIQYREMNSCLNADDLD).|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Scavenger receptor cysteine-rich type 1 protein M130|
|Molecular Weight||125451 MW|
|Protein Function||Acute phase-regulated receptor involved in clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages and may thereby protect tissues from free hemoglobin-mediated oxidative damage. May play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme. Binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH-dependent manner. Exhibits a higher affinity for complexes of hemoglobin and multimeric haptoglobin of HP*1F phenotype than for complexes of hemoglobin and dimeric haptoglobin of HP*1S phenotype. Induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production and secretion of IL6 and CSF1. Isoform 3 exhibits the higher capacity for ligand endocytosis and the more pronounced surface expression when expressed in cells.|
|Tissue Specificity||Expressed in monocytes and mature macrophages such as Kupffer cells in the liver, red pulp macrophages in the spleen, cortical macrophages in the thymus, resident bone marrow macrophages and meningeal macrophages of the central nervous system. Expressed also in blood. Isoform 1 is the lowest abundant in the blood. Isoform 2 is the lowest abundant in the liver and the spleen. Isoform 3 is the predominant isoform detected in the blood. .|
|Sequence Similarities||Contains 9 SRCR domains.|
|Subcellular Localization||Soluble CD163: Secreted.|
|Alternative Names||Scavenger receptor cysteine-rich type 1 protein M130;Hemoglobin scavenger receptor;CD163;Soluble CD163;sCD163;CD163;M130;|
|Research Areas|||immunology|innate immunity|macrophage / inflamm.| immunology|cell type markers|myeloid cells| cardiovascular|atherosclerosis|vascular inflammation|innate and adaptive immunity||
Background for Scavenger receptor cysteine-rich type 1 protein M130
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-CD163 Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Western blot, 0.1-0.5μg/ml, Human|
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-CD163 Antibody Images
Click the images to enlarge.
Recombinant Protein Detection Source: E.coli derived -recombinant human CD163, 35.2KD (162aa tag+E1002-L1156)
Lane 1: Recombinant Human CD163 Protein 10ng
Lane 2: Recombinant Human CD163 Protein 5ng
Lane 3: Recombinant Human CD163 Protein 2.5ng
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,