Product Info Summary
SKU: | M01025 |
---|---|
Size: | 100ug/vial |
Reactive Species: | Human |
Host: | Mouse |
Application: | Flow Cytometry, IF, IHC, WB |
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Product info
Product Name
Anti-CD209 / DC-SIGN (Pathogen Receptor on Dendritic Cells) Monoclonal Antibody
SKU/Catalog Number
M01025
Size
100ug/vial
Form
Liquid
Description
Boster Bio Anti-CD209 / DC-SIGN (Pathogen Receptor on Dendritic Cells) Monoclonal Antibody (Catalog # M01025). Tested in Flow Cytometry, IF, WB, IHC applications. This antibody reacts with Human.
Storage & Handling
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Cite This Product
Anti-CD209 / DC-SIGN (Pathogen Receptor on Dendritic Cells) Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01025)
Host
Mouse
Contents
Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Clonality
Monoclonal
Clone Number
Clone: C209/1781
Isotype
IgG2b, kappa
Immunogen
Recombinant human CD209 protein fragment (exact sequence is proprietary)
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Does not cross-react with primate, avian or amphibian GR.
Reactive Species
M01025 is reactive to CD209 in Human
Applications
M01025 is guaranteed for Flow Cytometry, IF, IHC, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
45.775kDa
Background of CD209
DC-SIGN is a transmembrane receptor that is expressed on the surface of dendritic cells and macrophages. It is involved in the innate immune system and recognizes numerous evolutionarily divergent pathogens ranging from parasites to viruses. The protein is organized into three distinct domains: an N-terminal transmembrane domain, a tandem-repeat neck domain and C-type lectin carbohydrate recognition domain. The extracellular region consisting of the C-type lectin and neck domains has a dual function as a pathogen recognition receptor and a cell adhesion receptor by binding carbohydrate ligands on the surface of microbes and endogenous cells. The neck region is important for homo-oligomerization, which allows the receptor to bind multivalent ligands with high avidity.Â
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Reconstitute with distilled water.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow Cytometry (0.5-1ug/million cells)
Immunofluorescence (1-2ug/ml)
Western Blotting (0.5-1ug/ml)
Immunohistochemistry (Formalin-fixed) (0.5-1ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Tris with 1mM EDTA Buffer, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.
Validation Images & Assay Conditions
Click image to see more details
Formalin-fixed, paraffin-embedded human Lung Carcinoma stained with Anti-CD209 Mouse Monoclonal Antibody (C209/1781).
Click image to see more details
Formalin-fixed, paraffin-embedded human Small Intestine stained with Anti-CD209 Mouse Monoclonal Antibody (C209/1781).
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Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Anti-CD209 Mouse Monoclonal Antibody (C209/1781).
Protein Target Info & Infographic
Gene/Protein Information For CD209 (Source: Uniprot.org, NCBI)
Gene Name
CD209
Full Name
CD209 antigen
Weight
45.775kDa
Alternative Names
CD209 antigendendritic cell-specific intracellular adhesion molecules (ICAM)-3 grabbingnon-integrin; CD209 molecule; CD209; CDSIGNHIV gpl20-binding protein; CLEC4L; CLEC4LC-type lectin domain family 4 member L; DCSIGN; DC-SIGN; DC-SIGN1; DC-SIGN1C-type lectin domain family 4, member L; DC-SIGNMGC129965; Dendritic cell-specific ICAM-3-grabbing non-integrin 1 CD209 CDSIGN, CLEC4L, DC-SIGN, DC-SIGN1 CD209 molecule CD209 antigen|C-type lectin domain family 4 member L|HIV gpl20-binding protein|dendritic cell-specific ICAM-3-grabbing non-integrin 1|dendritic cell-specific intercellular adhesion molecule-3-grabbing non-integrin|dendritic cell-specific intracellular adhesion molecules (ICAM)-3 grabbing non-integrin
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CD209, check out the CD209 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CD209: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CD209 / DC-SIGN (Pathogen Receptor on Dendritic Cells) Monoclonal Antibody (M01025)
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No publications found for M01025
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Recommended Resources
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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Submit your question
15 Customer Q&As for Anti-CD209 / DC-SIGN (Pathogen Receptor on Dendritic Cells) Monoclonal Antibody
Question
My boss were happy with the WB result of your anti-CD209 / DC-SIGN (Pathogen Receptor on Dendritic Cells) Monoclonal antibody. However we have observed positive staining in uterus isoform 1: cell membrane using this antibody. Is that expected? Could you tell me where is CD209 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-04-24
Answer
From literature, uterus does express CD209. Generally CD209 expresses in isoform 1: cell membrane. Regarding which tissues have CD209 expression, here are a few articles citing expression in various tissues:
Placenta, Pubmed ID: 1518869
Uterus, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2020-04-24
Question
Is there a BSA free version of anti-CD209 / DC-SIGN (Pathogen Receptor on Dendritic Cells) Monoclonal antibody M01025 available?
Verified Customer
Verified customer
Asked: 2020-04-06
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-CD209 / DC-SIGN (Pathogen Receptor on Dendritic Cells) Monoclonal antibody M01025 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-04-06
Question
My question regarding product M01025, anti-CD209 / DC-SIGN (Pathogen Receptor on Dendritic Cells) Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-03-24
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M01025 anti-CD209 / DC-SIGN (Pathogen Receptor on Dendritic Cells) Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-03-24
Question
Would anti-CD209 / DC-SIGN (Pathogen Receptor on Dendritic Cells) Monoclonal antibody M01025 work for IHC with placenta?
Verified Customer
Verified customer
Asked: 2019-12-17
Answer
According to the expression profile of placenta, CD209 is highly expressed in placenta. So, it is likely that anti-CD209 / DC-SIGN (Pathogen Receptor on Dendritic Cells) Monoclonal antibody M01025 will work for IHC with placenta.
Boster Scientific Support
Answered: 2019-12-17
Question
I see that the anti-CD209 / DC-SIGN (Pathogen Receptor on Dendritic Cells) Monoclonal antibody M01025 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-12-10
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-12-10
Question
My lab would like to test anti-CD209 / DC-SIGN (Pathogen Receptor on Dendritic Cells) Monoclonal antibody M01025 on human placenta for research purposes, then I may be interested in using anti-CD209 / DC-SIGN (Pathogen Receptor on Dendritic Cells) Monoclonal antibody M01025 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-11-27
Answer
The products we sell, including anti-CD209 / DC-SIGN (Pathogen Receptor on Dendritic Cells) Monoclonal antibody M01025, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-11-27
Question
I was wanting to use your anti-CD209 / DC-SIGN (Pathogen Receptor on Dendritic Cells) Monoclonal antibody for IHC for human placenta on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human placenta identification?
Verified Customer
Verified customer
Asked: 2018-08-31
Answer
It shows on the product datasheet, M01025 anti-CD209 / DC-SIGN (Pathogen Receptor on Dendritic Cells) Monoclonal antibody has been tested for IHC on human tissues. We have an innovator award program that if you test this antibody and show it works in human placenta in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-08-31
Question
We are currently using anti-CD209 / DC-SIGN (Pathogen Receptor on Dendritic Cells) Monoclonal antibody M01025 for human tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on dog tissues as well?
K. Krishna
Verified customer
Asked: 2018-07-04
Answer
The anti-CD209 / DC-SIGN (Pathogen Receptor on Dendritic Cells) Monoclonal antibody (M01025) has not been validated for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-07-04
Question
Would M01025 anti-CD209 / DC-SIGN (Pathogen Receptor on Dendritic Cells) Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-05-11
Answer
You can see on the product datasheet, M01025 anti-CD209 / DC-SIGN (Pathogen Receptor on Dendritic Cells) Monoclonal antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-05-11
Question
Would anti-CD209 / DC-SIGN (Pathogen Receptor on Dendritic Cells) Monoclonal antibody M01025 work on canine IHC with adrenal gland?
Verified Customer
Verified customer
Asked: 2018-03-09
Answer
Our lab technicians have not validated anti-CD209 / DC-SIGN (Pathogen Receptor on Dendritic Cells) Monoclonal antibody M01025 on canine. You can run a BLAST between canine and the immunogen sequence of anti-CD209 / DC-SIGN (Pathogen Receptor on Dendritic Cells) Monoclonal antibody M01025 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine adrenal gland in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-03-09
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for placenta using anti-CD209 / DC-SIGN (Pathogen Receptor on Dendritic Cells) Monoclonal antibody M01025. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2017-10-25
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-10-25
Question
Is a blocking peptide available for product anti-CD209 / DC-SIGN (Pathogen Receptor on Dendritic Cells) Monoclonal antibody (M01025)?
A. Johnson
Verified customer
Asked: 2017-04-07
Answer
We do provide the blocking peptide for product anti-CD209 / DC-SIGN (Pathogen Receptor on Dendritic Cells) Monoclonal antibody (M01025). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2017-04-07
Question
We have been able to see staining in human adrenal gland. Any tips? Is anti-CD209 / DC-SIGN (Pathogen Receptor on Dendritic Cells) Monoclonal antibody supposed to stain adrenal gland positively?
A. Li
Verified customer
Asked: 2015-04-24
Answer
From what I have seen in literature adrenal gland does express CD209. From what I have seen in Uniprot.org, CD209 is expressed in adrenal gland, placenta, uterus, among other tissues. Regarding which tissues have CD209 expression, here are a few articles citing expression in various tissues:
Placenta, Pubmed ID: 1518869
Uterus, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2015-04-24
Question
Is this M01025 anti-CD209 / DC-SIGN (Pathogen Receptor on Dendritic Cells) Monoclonal antibody reactive to the isotypes of CD209?
P. Anderson
Verified customer
Asked: 2015-02-23
Answer
The immunogen of M01025 anti-CD209 / DC-SIGN (Pathogen Receptor on Dendritic Cells) Monoclonal antibody is Recombinant human CD209 protein fragment. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2015-02-23
Question
Here is the WB image, lot number and protocol we used for placenta using anti-CD209 / DC-SIGN (Pathogen Receptor on Dendritic Cells) Monoclonal antibody M01025. Please let me know if you require anything else.
G. Singh
Verified customer
Asked: 2014-09-22
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2014-09-22