Product Info Summary
SKU: | A01148-1 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Rat |
Host: | Rabbit |
Application: | Flow Cytometry, IHC, ICC, WB |
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Product info
Product Name
Anti-CD27 antigen CD27 Antibody
View all CD27/TNFRSF7 Antibodies
SKU/Catalog Number
A01148-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-CD27 antigen CD27 Antibody catalog # A01148-1. Tested in WB,ICC,IHC,Flow Cytometry applications. This antibody reacts with Human,rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD27 antigen CD27 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01148-1)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthetic peptide, corresponding to amino acids 91-140 of Human IL-1α.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A01148-1 is reactive to CD27 in Human, Rat
Applications
A01148-1 is guaranteed for Flow Cytometry, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
29.137kDa
Background of CD27/TNFRSF7
This protein mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which Na+ ions may pass in accordance with their electrochemical gradient. This sodium channel may be present in both denervated and innervated skeletal muscle.
George A.L. Jr., Ann. Neurol. 31:131-137(1992).
Wang J., Biochem. Biophys. Res. Commun. 182:794-801(1992).
Tsujino A., Proc. Natl. Acad. Sci. U.S.A. 100:7377-7382(2003).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:1,000
IHC: 1:50-1:200
ICC: 1:50-1:200
FC: 1:50-1:100
Validation Images & Assay Conditions
Click image to see more details
Figure 2. Immunocytochemistry staining of CD27 using Anti-CD27 antigen CD27 Antibody (A01148-1).
ICC staining CD27 in K562 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehydeClick image to see more details
Figure 1. Western blotting validation for Anti-CD27 antigen CD27 Antibody A01148-1
Western blot analysis of CD27 on Raji cell using anti-CD27 antibody at 1/500 dilution.Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For CD27 (Source: Uniprot.org, NCBI)
Gene Name
CD27
Full Name
CD27 antigen
Weight
29.137kDa
Alternative Names
CD27 antigen; CD27 molecule; CD27; MGC20393; S152CD27L receptor; T cell activation antigen CD27; T cell activation antigen S152; T14; TNFRSF7; TNFRSF7T-cell activation antigen CD27; Tp55; Tumor necrosis factor receptor superfamily member 7; tumor necrosis factor receptor superfamily, member 7 CD27 S152, S152. LPFS2, T14, TNFRSF7, Tp55 CD27 molecule CD27 antigen|CD27L receptor|T cell activation antigen S152|T-cell activation antigen CD27|tumor necrosis factor receptor superfamily, member 7
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CD27, check out the CD27 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CD27: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CD27 antigen CD27 Antibody (A01148-1)
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No publications found for A01148-1
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