Anti-CD27 (Tumor Necrosis Factor Receptor Superfamily 7) Monoclonal Antibody

CD27/TNFRSF7 antibody

Boster Bio Anti-CD27 (Tumor Necrosis Factor Receptor Superfamily 7) Monoclonal Antibody catalog # M01148. Tested in IHC applications. This antibody reacts with Human.

Product Info Summary

SKU: M01148
Size: 100ug/vial
Reactive Species: Human
Host: Mouse
Application: IHC

Product Name

Anti-CD27 (Tumor Necrosis Factor Receptor Superfamily 7) Monoclonal Antibody

View all CD27/TNFRSF7 Antibodies

SKU/Catalog Number

M01148

Size

100ug/vial

Form

Liquid

Description

Boster Bio Anti-CD27 (Tumor Necrosis Factor Receptor Superfamily 7) Monoclonal Antibody catalog # M01148. Tested in IHC applications. This antibody reacts with Human.

Storage & Handling

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Cite This Product

Anti-CD27 (Tumor Necrosis Factor Receptor Superfamily 7) Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01148)

Host

Mouse

Contents

Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Clonality

Monoclonal

Clone Number

LPFS2/1611

Isotype

IgG1, kappa

Immunogen

Recombinant human CD22 protein (exact sequence is proprietary)

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M01148 is reactive to CD27 in Human

Applications

M01148 is guaranteed for IHC Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

View more details

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists world wide.

Submit A Review

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Immunohistology (Formalin-fixed) (0.5-1ug/ml for 30 minutes at RT)
Immunofluorescence (0.5-1ug/ml)

Validation Images & Assay Conditions

Gene/Protein Information For CD27 (Source: Uniprot.Org, NCBI)

Uniprot ID

P26842

Gene ID

939

Gene Name

CD27

Full Name

CD27 antigen

Weight

29.137kDa

Alternative Names

CD27 antigen; CD27 molecule; CD27; MGC20393; S152CD27L receptor; T cell activation antigen CD27; T cell activation antigen S152; T14; TNFRSF7; TNFRSF7T-cell activation antigen CD27; Tp55; Tumor necrosis factor receptor superfamily member 7; tumor necrosis factor receptor superfamily, member 7 CD27 S152, S152. LPFS2, T14, TNFRSF7, Tp55 CD27 molecule CD27 antigen|CD27L receptor|T cell activation antigen S152|T-cell activation antigen CD27|tumor necrosis factor receptor superfamily, member 7

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on CD27, check out the CD27 Infographic

CD27 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for CD27: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

No publications found for M01148

*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.

Have you used Anti-CD27 (Tumor Necrosis Factor Receptor Superfamily 7) Monoclonal Antibody?

Submit a review and receive an Amazon gift card.

  • $25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
  • $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit A Review

Be the first to review Anti-CD27 (Tumor Necrosis Factor Receptor Superfamily 7) Monoclonal Antibody

*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.

Have a question?

Find answers in Q&As, reviews.

Can't find your answer?

Submit your question

3 Customer Q&As for Anti-CD27 (Tumor Necrosis Factor Receptor Superfamily 7) Monoclonal Antibody

Question

We have observed staining in human b-cell. Are there any suggestions? Is anti-CD27 (Tumor Necrosis Factor Receptor Superfamily 7) Monoclonal antibody supposed to stain b-cell positively?

Verified Customer

Verified customer

Asked: 2020-02-07

Answer

From literature b-cell does express CD27. From Uniprot.org, CD27 is expressed in lymph node, monocyte, thymus, b-cell, cervix carcinoma thymus, among other tissues. Regarding which tissues have CD27 expression, here are a few articles citing expression in various tissues:
B-cell, Pubmed ID: 15489334
Cervix carcinoma, and Thymus, Pubmed ID: 9177220
Monocyte, Pubmed ID: 1655907
Thymus, Pubmed ID: 14702039

Boster Scientific Support

Answered: 2020-02-07

Question

We are currently using anti-CD27 (Tumor Necrosis Factor Receptor Superfamily 7) Monoclonal antibody M01148 for human tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on horse tissues as well?

Verified Customer

Verified customer

Asked: 2019-10-09

Answer

The anti-CD27 (Tumor Necrosis Factor Receptor Superfamily 7) Monoclonal antibody (M01148) has not been validated for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-10-09

Question

We were content with the WB result of your anti-CD27 (Tumor Necrosis Factor Receptor Superfamily 7) Monoclonal antibody. However we have seen positive staining in lymph node membrane using this antibody. Is that expected? Could you tell me where is CD27 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2019-08-19

Answer

According to literature, lymph node does express CD27. Generally CD27 expresses in membrane. Regarding which tissues have CD27 expression, here are a few articles citing expression in various tissues:
B-cell, Pubmed ID: 15489334
Cervix carcinoma, and Thymus, Pubmed ID: 9177220
Monocyte, Pubmed ID: 1655907
Thymus, Pubmed ID: 14702039

Boster Scientific Support

Answered: 2019-08-19

Size

Total: $440

SKU:M01148

Ask a question

Get A Quote

Ships directly to USA and Canada. For other regions, visit our distributors page

Bulk Inquiry

Product Categories

Primary Antibodies

Primary and Secondary Antibodies

Boster Bio offers over 16,000 antibodies that have been validated for IHC, WB, ELISA, and FC in human, mouse, and rat samples. Rabbit and mouse monoclonal antibodies as well as rabbit polyclonal antibodies are available. Buy a primary antibody and get a secondary antibody for free!

ELISA Kits

ELISA Kits

More than 1,000 ELISA kits, both singleplex and multiplex, that have been cited 6,000+ times are available at Boster. We offer our Boster-branded Picokine™ ELISA kits (sandwich ELISA) and EZ-Set™ ELISA kits (DIY antibody pairs) in addition to many multiplex ELISA kits.

Image

Recombinant Proteins

Boster provides a wide range of human, mouse, and rat recombinant proteins, which include cytokines, growth factors, chemokines, and many more. Our recombinant proteins have high stability, biological activity, and purity.

Boster Kit

Reagents

Boster offers all the reagents you need for your immunostaining and western blotting experiments. We've got everything from buffers to lysates to kits, including our popular and well-cited BCA, CCK-8, and MTT assay kits.

In stock
Order Product
M01148
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$440.00

Troubleshooting

troubleshooting-box-image

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

Guaranteed product quality

We promise all of our products perform as described in datasheets.