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Product Info Summary
| SKU: | M01148 |
|---|---|
| Size: | 100ug/vial |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | IHC |
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Product info
Product Name
Anti-CD27 (Tumor Necrosis Factor Receptor Superfamily 7) Monoclonal Antibody
View all CD27/TNFRSF7 Antibodies
SKU/Catalog Number
M01148
Size
100ug/vial
Form
Liquid
Description
Boster Bio Anti-CD27 (Tumor Necrosis Factor Receptor Superfamily 7) Monoclonal Antibody catalog # M01148. Tested in IHC applications. This antibody reacts with Human.
Storage & Handling
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Cite This Product
Anti-CD27 (Tumor Necrosis Factor Receptor Superfamily 7) Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01148)
Host
Mouse
Contents
Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Clonality
Monoclonal
Clone Number
LPFS2/1611
Isotype
IgG1, kappa
Immunogen
Recombinant human CD22 protein (exact sequence is proprietary)
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01148 is reactive to CD27 in Human
Applications
M01148 is guaranteed for IHC Boster Guarantee
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists world wide.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
Immunohistology (Formalin-fixed) (0.5-1ug/ml for 30 minutes at RT)
Immunofluorescence (0.5-1ug/ml)
Validation Images & Assay Conditions
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Formalin-fixed, paraffin-embedded human Tonsil stained withCD27 Monoclonal Antibody (LPFS2/1611).
Protein Target Info & Infographic
Gene/Protein Information For CD27 (Source: Uniprot.Org, NCBI)
Uniprot ID
P26842
Gene ID
939
Gene Name
CD27
Full Name
CD27 antigen
Weight
29.137kDa
Alternative Names
CD27 antigen; CD27 molecule; CD27; MGC20393; S152CD27L receptor; T cell activation antigen CD27; T cell activation antigen S152; T14; TNFRSF7; TNFRSF7T-cell activation antigen CD27; Tp55; Tumor necrosis factor receptor superfamily member 7; tumor necrosis factor receptor superfamily, member 7 CD27 S152, S152. LPFS2, T14, TNFRSF7, Tp55 CD27 molecule CD27 antigen|CD27L receptor|T cell activation antigen S152|T-cell activation antigen CD27|tumor necrosis factor receptor superfamily, member 7
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on CD27, check out the CD27 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for CD27: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
Specific Publications For Anti-CD27 (Tumor Necrosis Factor Receptor Superfamily 7) Monoclonal Antibody (M01148)
No publications found for M01148
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
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Customer Reviews
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Customer Q&As
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3 Customer Q&As for Anti-CD27 (Tumor Necrosis Factor Receptor Superfamily 7) Monoclonal Antibody
Question
We have observed staining in human b-cell. Are there any suggestions? Is anti-CD27 (Tumor Necrosis Factor Receptor Superfamily 7) Monoclonal antibody supposed to stain b-cell positively?
Verified Customer
Verified customer
Asked: 2020-02-07
Answer
From literature b-cell does express CD27. From Uniprot.org, CD27 is expressed in lymph node, monocyte, thymus, b-cell, cervix carcinoma thymus, among other tissues. Regarding which tissues have CD27 expression, here are a few articles citing expression in various tissues:
B-cell, Pubmed ID: 15489334
Cervix carcinoma, and Thymus, Pubmed ID: 9177220
Monocyte, Pubmed ID: 1655907
Thymus, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2020-02-07
Question
We are currently using anti-CD27 (Tumor Necrosis Factor Receptor Superfamily 7) Monoclonal antibody M01148 for human tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2019-10-09
Answer
The anti-CD27 (Tumor Necrosis Factor Receptor Superfamily 7) Monoclonal antibody (M01148) has not been validated for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-10-09
Question
We were content with the WB result of your anti-CD27 (Tumor Necrosis Factor Receptor Superfamily 7) Monoclonal antibody. However we have seen positive staining in lymph node membrane using this antibody. Is that expected? Could you tell me where is CD27 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-08-19
Answer
According to literature, lymph node does express CD27. Generally CD27 expresses in membrane. Regarding which tissues have CD27 expression, here are a few articles citing expression in various tissues:
B-cell, Pubmed ID: 15489334
Cervix carcinoma, and Thymus, Pubmed ID: 9177220
Monocyte, Pubmed ID: 1655907
Thymus, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2019-08-19
