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SKU:MA1015
Pack Size:100μg/vial
Sample Size:30ug for $99, contact us for details
Clonality:Monoclonal
Clone Number:CA-3
Application:IHC-F
Price: $200.00
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Overview

Product Name Anti-CD3 Antibody (Monoclonal, CA-3)
SKU/Catalog Number MA1015
Description Mouse IgG monoclonal antibody for CD3, CD3e molecule, epsilon (CD3-TCR complex) (CD3E ) detection. Tested with IHC-F in Human. No cross reactivity with other proteins.
Cite This Product Anti-CD3 Antibody (Monoclonal, CA-3) CA-3 (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1015)
Replacement Item This antibody may replace the following items: sc-5765|sc-59614|sc-5767|sc-20768|sc-376262|sc-20768|sc-376262|sc-5765|sc-5767|sc-59614 from Santa Cruz Biotechnology.
Host Mouse
Isotype Mouse IgG1
Validated Species Human
Application IHC-F

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(F).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen Human thymocytes followed by Sezary T cells.
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Monoclonal
Clone Number CA-3
Form Lyophilized
Contents Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
*carrier free antibody available upon request.
Concentration Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Ascites
Isotype Mouse IgG1

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name Cd3e
Protein Name T-cell surface glycoprotein CD3 epsilon chain
Molecular Weight 21312 MW
Protein Function CD3E: The CD3 complex mediates signal transduction.
Sequence Similarities Contains 1 Ig-like (immunoglobulin-like) domain.
Subcellular Localization CD3E: Membrane; Single-pass type I membraneprotein.
Cd3e: Cell membrane
Uniprot ID D4A5M2
Alternative Names CD3 antigen, epsilon polypeptide (Predicted) ;Protein Cd3e ;Cd3e ;Cd3e_predicted ;rCG_58471 ;
Research Areas |immunology|adaptive immunity|t cells| stem cells|hematopoietic progenitors|hematopoietic stem cells|human lineage negative| immunology|regulatory t cells|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for T-cell surface glycoprotein CD3 epsilon chain

The alpha-beta heterodimeric T-cell antigen receptor binds antigen in association with major histocompatibility complex proteins on host cell surfaces. These 2 disulfide-linked glycoproteins, TCRA and TCRB are associated on T-cell surfaces with a complex of proteins called CD3(formerly T3). Human CD3 consists of at least 4 proteins: gamma, delta, epsilon, and zeta. The CD3-gamma/CD3-delta gene pair is within 300 kb of the CD3-epsilon gene and therefore these genes form a tightly linked cluster on 11q23. The clustering may be significant in terms of their simultaneous activations during T-cell development.
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Publications

Chen B, Liu L, Xu H, Yang Y, Zhang L, Zhang F. Exp Ther Med. 2015 Mar;9(3):1063-1067. Epub 2015 Jan 26. Effectiveness Of Immune Therapy Combined With Chemotherapy On The Immune Function And Recurrence Rate Of Cervical Cancer.
Yu X, Li L, Li Q, Zang X, Liu Z. Biol Trace Elem Res. 2011 Nov;143(2):1064-76. Doi: 10.1007/S12011-010-8941-5. Epub 2011 Jan 12. Trail And Dr5 Promote Thyroid Follicular Cell Apoptosis In Iodine Excess-Induced Experimental Autoimmune Thyroiditis I...
Zhang R, Zhou Sj, Li Cj, Wang Xn, Tang Yz, Chen R, Lv L, Zhao Q, Xing Ql, Yu Dm, Yu P. Lipids Health Dis. 2013 Mar 26;12:42. Doi: 10.1186/1476-511X-12-42. C-Reactive Protein/Oxidised Low-Density Lipoprotein/??2-Glycoprotein I Complex Promotes Ather...
Huang J, Zhu C, Zhang P, Zhu Q, Liu Y, Zhu Z, Wang M, Li W, Yang G, Dong N, Liu J, Chen L, Zhang Y, Yang R, Deng L, Fan J, Wang X, Liu J, Ma B, Fu Q, Wu K. Sci Rep. 2013;3:1114. Doi: 10.1038/Srep01114. Epub 2013 Jan 23. S100+ Cells: A New Neuro-Im...
Xie Zj, Jia Lm, He Yc, Gao Jt. World J Gastroenterol. 2006 Mar 21;12(11):1757-60. Morphological Observation Of Tumor Infiltrating Immunocytes In Human Rectal Cancer.
Li Y, Geng S, Yin Q, Chen S, Yang L, Wu X, Li B, Du X, Schmidt Ca, Przybylski Gk. J Transl Med. 2010 May 14;8:47. Doi: 10.1186/1479-5876-8-47. Decreased Level Of Recent Thymic Emigrants In Cd4+ And Cd8+T Cells From Cml Patients.
Li Y, Geng S, Du X, Chen S, Yang L, Wu X, Li B, Schmidt Ca, Przybylski Gk. Hematology. 2011 Jan;16(1):43-9. Doi: 10.1179/102453311X12902908411634. Restricted Trbv Repertoire In Cd4+ And Cd8+ T-Cell Subsets From Cml Patients.
Liu Yz, Cao Yg, Ye Jq, Wang Wg, Song Kj, Wang Xl, Wang Ch, Li Rt, Deng Xm. Fitoterapia. 2010 Mar;81(2):108-14. Doi: 10.1016/J.Fitote.2009.08.005. Epub 2009 Aug 15. Immunomodulatory Effects Of Proanthocyanidin A-1 Derived In Vitro From Rhododendron...
Jia H, Wang Y, Tong Xj, Liu Gb, Li Q, Zhang Lx, Sun Xh. Artif Cells Blood Substit Immobil Biotechnol. 2011 Dec;39(6):366-75. Doi: 10.3109/10731199.2011.618133. Epub 2011 Oct 14. Biocompatibility Of Acellular Nerves Of Different Mammalian Species F...

FAQs

Q: What would be the approximate optimal dilution for this antibody? Can we also use this antibody on paraffin embedded sections? Keyword: protocol, applications
A: The antibodies are lyophilized, and the recommended working concentration is 1μg/ml. This antibody can only be used on frozen section.
Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: Can you provide monoclonal antibodies carrier free?
A: We cannot.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to cd3 antibody, t cell receptor antibody, t3 antibody