|Application:||IHC-P, IHC-F, ICC, WB|
Data & Images
|Product Name||Anti-CD3 Epsilon Picoband™ Antibody|
|Description||Rabbit IgG polyclonal antibody for T-cell surface glycoprotein CD3 epsilon chain(CD3E) detection. Tested with WB, IHC-P, IHC-F, ICC in Human;Mouse;Rat.|
|Cite This Product||Anti-CD3 Epsilon Picoband™ Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9093)|
|Replacement Item||This antibody may replace the following items: sc-1127|sc-1179|sc-137095|sc-137096|sc-137234|sc-20047|sc-20779|sc-20918|sc-26249|sc-32324|sc-367515|sc-367515-X|sc-376547|sc-393781|sc-393781-X|sc-393818|sc-393818-X|sc-398494|sc-398494-X|sc-70271|sc-70272|sc-70273|sc-70486|sc-7560|sc-81172|sc-82549|sc-82549-X from Santa Cruz Biotechnology.|
|Validated Species||Human, Mouse, Rat|
|Application||IHC-P, IHC-F, ICC, WB
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P), IHC(F) and ICC.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||E.coli-derived human CD3 epsilon recombinant protein (Position: D23-I207). Human CD3 epsilon shares 65% amino acid (aa) sequence identity with mouse CD3 epsilon.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
*carrier free antibody available upon request.
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||T-cell surface glycoprotein CD3 epsilon chain|
|Molecular Weight||23147 MW|
|Protein Function||The CD3 complex mediates signal transduction.|
|Sequence Similarities||Contains 1 Ig-like (immunoglobulin-like) domain.|
|Subcellular Localization||Membrane; Single-pass type I membrane protein.|
|Alternative Names||T-cell surface glycoprotein CD3 epsilon chain;T-cell surface antigen T3/Leu-4 epsilon chain;CD3e;CD3E;T3E;|
|Research Areas|||immunology|adaptive immunity|t cells| stem cells|hematopoietic progenitors|hematopoietic stem cells|human lineage negative| immunology|regulatory t cells||
Background for T-cell surface glycoprotein CD3 epsilon chain
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-CD3 Epsilon Picoband™ Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunocytochemistry , 0.5-1μg/ml, Human, -|
Immunohistochemistry(Frozen Section), 0.5-1μg/ml, Mouse, Rat, -
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Western blot, 0.1-0.5μg/ml, Human
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-CD3 Epsilon Picoband™ Antibody Images
Click the images to enlarge.
All lanes: Anti CD3 Epsilon (PB9093) at 0.5ug/ml
WB : Recombinant Human CD3epsilon Protein 0.5ng
Predicted bind size: 40KD
Observed bind size: 40KD
All lanes: Anti CD3 Epsilon (PB9093) at 0.5ug/ml
Lane 1: JURKAT Whole Cell Lysate at 40ug
Lane 2: CEM Whole Cell Lysate at 40ug
Predicted bind size: 23KD
Observed bind size: 23KD
IHC(P): Human Tonsil Tissue
IHC(P): Mouse Spleen Tissue
IHC(P): Rat Spleen Tissue
IHC(F): Rat Spleen Tissue
IHC(F): Mouse Spleen Tissue
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,