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SKU:PB9093
Pack Size:100μg/vial
Sample Size:30ug for $99, contact us for details
Clonality:Polyclonal
Application:IHC-P, IHC-F, ICC, WB
Price: $240.00
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Overview

Product Name Anti-CD3 Epsilon Picoband™ Antibody
SKU/Catalog Number PB9093
Description Rabbit IgG polyclonal antibody for T-cell surface glycoprotein CD3 epsilon chain(CD3E) detection. Tested with WB, IHC-P, IHC-F, ICC in Human;Mouse;Rat.
Cite This Product Anti-CD3 Epsilon Picoband™ Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9093)
Replacement Item This antibody may replace the following items: sc-1127|sc-1179|sc-137095|sc-137096|sc-137234|sc-20047|sc-20779|sc-20918|sc-26249|sc-32324|sc-367515|sc-367515-X|sc-376547|sc-393781|sc-393781-X|sc-393818|sc-393818-X|sc-398494|sc-398494-X|sc-70271|sc-70272|sc-70273|sc-70486|sc-7560|sc-81172|sc-82549|sc-82549-X from Santa Cruz Biotechnology.
Host Rabbit
Isotype N/A
Validated Species Human, Mouse, Rat
Application IHC-P, IHC-F, ICC, WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P), IHC(F) and ICC.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen E.coli-derived human CD3 epsilon recombinant protein (Position: D23-I207). Human CD3 epsilon shares 65% amino acid (aa) sequence identity with mouse CD3 epsilon.
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Polyclonal
Form Lyophilized
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
*carrier free antibody available upon request.
Concentration Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Immunogen affinity purified.
Isotype N/A

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name CD3E
Protein Name T-cell surface glycoprotein CD3 epsilon chain
Molecular Weight 23147 MW
Protein Function The CD3 complex mediates signal transduction.
Sequence Similarities Contains 1 Ig-like (immunoglobulin-like) domain.
Subcellular Localization Membrane; Single-pass type I membrane protein.
Uniprot ID P07766
Alternative Names T-cell surface glycoprotein CD3 epsilon chain;T-cell surface antigen T3/Leu-4 epsilon chain;CD3e;CD3E;T3E;
Research Areas |immunology|adaptive immunity|t cells| stem cells|hematopoietic progenitors|hematopoietic stem cells|human lineage negative| immunology|regulatory t cells|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for T-cell surface glycoprotein CD3 epsilon chain

CD3e molecule, epsilon also known as CD3E is a polypeptide which in humans is encoded by the CD3E gene which resides on chromosome 11. It is mapped to 11q23.3. The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development.

Anti-CD3 Epsilon Picoband™ Antibody Images

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Anti-CD3 Epsilon Picoband™ Antibody
Anti-CD3 epsilon Picoband antibody, PB9093-1.jpg
All lanes: Anti CD3 Epsilon (PB9093) at 0.5ug/ml
WB : Recombinant Human CD3epsilon Protein 0.5ng
Predicted bind size: 40KD
Observed bind size: 40KD
Anti-CD3 Epsilon Picoband™ Antibody
Anti-CD3 epsilon Picoband antibody, PB9093-2.jpg
All lanes: Anti CD3 Epsilon (PB9093) at 0.5ug/ml
Lane 1: JURKAT Whole Cell Lysate at 40ug
Lane 2: CEM Whole Cell Lysate at 40ug
Predicted bind size: 23KD
Observed bind size: 23KD
Anti-CD3 Epsilon Picoband™ Antibody
Anti-CD3 epsilon Picoband antibody, PB9093-3.JPG
IHC(P): Human Tonsil Tissue
Anti-CD3 Epsilon Picoband™ Antibody
Anti-CD3 epsilon Picoband antibody, PB9093-4.JPG
IHC(P): Mouse Spleen Tissue
Anti-CD3 Epsilon Picoband™ Antibody
Anti-CD3 epsilon Picoband antibody, PB9093-5.JPG
IHC(P): Rat Spleen Tissue
Anti-CD3 Epsilon Picoband™ Antibody
Anti-CD3 epsilon Picoband antibody, pb9093-6.JPG
IHC(F): Rat Spleen Tissue
Anti-CD3 Epsilon Picoband™ Antibody
Anti-CD3 epsilon Picoband antibody, pb9093-7.JPG
IHC(F): Mouse Spleen Tissue
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FAQs

Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
A: You can find the immunogen sequence under "Immunogen" and clonality in the product name.
Q: What is the expected band size? Why is it different than the observed band size?
A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands

3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.

4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
A: Check our protocols under the tech support tab.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to cd3 epsilon antibody, cd3e antibody