Anti-CD30 / TNFRSF8 (Hodgkin & Reed-Sternberg Cell Marker) Monoclonal Antibody

Boster Bio Anti-CD30 / TNFRSF8 (Hodgkin & Reed-Sternberg Cell Marker) Monoclonal Antibody catalog # M01225. Tested in IHC applications. This antibody reacts with Human.

Product Info Summary

SKU: M01225
Size: 100ug/vial
Reactive Species: Human
Host: Mouse
Application: IHC

Product Name

Anti-CD30 / TNFRSF8 (Hodgkin & Reed-Sternberg Cell Marker) Monoclonal Antibody

See all CD30/TNFRSF8 products

SKU/Catalog Number

M01225

Size

100ug/vial

Form

Liquid

Description

Boster Bio Anti-CD30 / TNFRSF8 (Hodgkin & Reed-Sternberg Cell Marker) Monoclonal Antibody catalog # M01225. Tested in IHC applications. This antibody reacts with Human.

Storage & Handling

Cite This Product

Anti-CD30 / TNFRSF8 (Hodgkin & Reed-Sternberg Cell Marker) Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01225)

Host

Mouse

Contents

Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Clonality

Monoclonal

Clone Number

SPM609

Immunogen

Recombinant human TNFRSF8 protein

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M01225 is reactive to TNFRSF8 in Human

Applications

M01225 is guaranteed for IHC Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Immunohistology (Formalin-fixed) (0.5-1ug/ml for 30 minutes at RT)
Immunofluorescence (1-2ug/ml)

Validation Images & Assay Conditions

Gene/Protein Information For TNFRSF8 (Source: Uniprot.Org, NCBI)

Uniprot ID

P28908

Gene ID

943

Gene Name

TNFRSF8

Full Name

Tumor necrosis factor receptor superfamily member 8

Weight

63.747kDa

Superfamily

TNFR8 family

Alternative Names

CD30 antigen; CD30; CD30KI-1; CD30L receptor; cytokine receptor CD30; D1S166EKi-1; Ki-1 antigen; Lymphocyte activation antigen CD30; TNFRSF8; tumor necrosis factor receptor superfamily member 8; tumor necrosis factor receptor superfamily, member 8 TNFRSF8 CD30, D1S166E, Ki-1 TNF receptor superfamily member 8 tumor necrosis factor receptor superfamily member 8|CD30L receptor|Ki-1 antigen|cytokine receptor CD30|lymphocyte activation antigen CD30

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on TNFRSF8, check out the TNFRSF8 Infographic

TNFRSF8 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for TNFRSF8: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

No publications found for M01225

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15 Customer Q&As for Anti-CD30 / TNFRSF8 (Hodgkin & Reed-Sternberg Cell Marker) Monoclonal Antibody

Question

Is there a BSA free version of anti-CD30 / TNFRSF8 (Hodgkin & Reed-Sternberg Cell Marker) Monoclonal antibody M01225 available?

Verified Customer

Verified customer

Asked: 2020-01-02

Answer

Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-CD30 / TNFRSF8 (Hodgkin & Reed-Sternberg Cell Marker) Monoclonal antibody M01225 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2020-01-02

Question

See below the WB image, lot number and protocol we used for leukocyte using anti-CD30 / TNFRSF8 (Hodgkin & Reed-Sternberg Cell Marker) Monoclonal antibody M01225. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-12-24

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-12-24

Question

Is a blocking peptide available for product anti-CD30 / TNFRSF8 (Hodgkin & Reed-Sternberg Cell Marker) Monoclonal antibody (M01225)?

Verified Customer

Verified customer

Asked: 2019-08-20

Answer

We do provide the blocking peptide for product anti-CD30 / TNFRSF8 (Hodgkin & Reed-Sternberg Cell Marker) Monoclonal antibody (M01225). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-08-20

Question

Would M01225 anti-CD30 / TNFRSF8 (Hodgkin & Reed-Sternberg Cell Marker) Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-07-10

Answer

As indicated on the product datasheet, M01225 anti-CD30 / TNFRSF8 (Hodgkin & Reed-Sternberg Cell Marker) Monoclonal antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-07-10

Question

I was wanting to use your anti-CD30 / TNFRSF8 (Hodgkin & Reed-Sternberg Cell Marker) Monoclonal antibody for IHC for human leukocyte on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human leukocyte identification?

Verified Customer

Verified customer

Asked: 2019-07-05

Answer

You can see on the product datasheet, M01225 anti-CD30 / TNFRSF8 (Hodgkin & Reed-Sternberg Cell Marker) Monoclonal antibody has been validated for IHC on human tissues. We have an innovator award program that if you test this antibody and show it works in human leukocyte in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-07-05

Question

I see that the anti-CD30 / TNFRSF8 (Hodgkin & Reed-Sternberg Cell Marker) Monoclonal antibody M01225 works with IHC, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-06-24

Answer

You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-06-24

Question

We were well pleased with the WB result of your anti-CD30 / TNFRSF8 (Hodgkin & Reed-Sternberg Cell Marker) Monoclonal antibody. However we have observed positive staining in leukemic t-cell isoform 1: cell membrane using this antibody. Is that expected? Could you tell me where is TNFRSF8 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2019-05-24

Answer

From literature, leukemic t-cell does express TNFRSF8. Generally TNFRSF8 expresses in isoform 1: cell membrane, isoform 2: cytoplasm. Regarding which tissues have TNFRSF8 expression, here are a few articles citing expression in various tissues:
Blood, and Testis, Pubmed ID: 15489334
Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19690332
Lymphoid tissue, Pubmed ID: 1310894
Placenta, Pubmed ID: 16710414

Boster Scientific Support

Answered: 2019-05-24

Question

We have observed staining in human placenta. What should we do? Is anti-CD30 / TNFRSF8 (Hodgkin & Reed-Sternberg Cell Marker) Monoclonal antibody supposed to stain placenta positively?

Verified Customer

Verified customer

Asked: 2019-03-28

Answer

Based on literature placenta does express TNFRSF8. Based on Uniprot.org, TNFRSF8 is expressed in leukocyte, lymphoid tissue, placenta, blood testis, leukemic t-cell, erythroleukemia, among other tissues. Regarding which tissues have TNFRSF8 expression, here are a few articles citing expression in various tissues:
Blood, and Testis, Pubmed ID: 15489334
Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19690332
Lymphoid tissue, Pubmed ID: 1310894
Placenta, Pubmed ID: 16710414

Boster Scientific Support

Answered: 2019-03-28

Question

Does anti-CD30 / TNFRSF8 (Hodgkin & Reed-Sternberg Cell Marker) Monoclonal antibody M01225 work for IHC with leukocyte?

G. Gonzalez

Verified customer

Asked: 2019-03-28

Answer

According to the expression profile of leukocyte, TNFRSF8 is highly expressed in leukocyte. So, it is likely that anti-CD30 / TNFRSF8 (Hodgkin & Reed-Sternberg Cell Marker) Monoclonal antibody M01225 will work for IHC with leukocyte.

Boster Scientific Support

Answered: 2019-03-28

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for leukocyte using anti-CD30 / TNFRSF8 (Hodgkin & Reed-Sternberg Cell Marker) Monoclonal antibody M01225. Let me know if you need anything else.

A. Singh

Verified customer

Asked: 2018-11-30

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2018-11-30

Question

Is this M01225 anti-CD30 / TNFRSF8 (Hodgkin & Reed-Sternberg Cell Marker) Monoclonal antibody reactive to the isotypes of TNFRSF8?

E. Taylor

Verified customer

Asked: 2018-11-19

Answer

The immunogen of M01225 anti-CD30 / TNFRSF8 (Hodgkin & Reed-Sternberg Cell Marker) Monoclonal antibody is Recombinant human TNFRSF8 protein. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2018-11-19

Question

We are currently using anti-CD30 / TNFRSF8 (Hodgkin & Reed-Sternberg Cell Marker) Monoclonal antibody M01225 for human tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on canine tissues as well?

Verified Customer

Verified customer

Asked: 2018-01-04

Answer

The anti-CD30 / TNFRSF8 (Hodgkin & Reed-Sternberg Cell Marker) Monoclonal antibody (M01225) has not been validated for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2018-01-04

Question

I have a question about product M01225, anti-CD30 / TNFRSF8 (Hodgkin & Reed-Sternberg Cell Marker) Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

J. Carter

Verified customer

Asked: 2016-05-23

Answer

We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M01225 anti-CD30 / TNFRSF8 (Hodgkin & Reed-Sternberg Cell Marker) Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2016-05-23

Question

My lab would like to test anti-CD30 / TNFRSF8 (Hodgkin & Reed-Sternberg Cell Marker) Monoclonal antibody M01225 on human leukocyte for research purposes, then I may be interested in using anti-CD30 / TNFRSF8 (Hodgkin & Reed-Sternberg Cell Marker) Monoclonal antibody M01225 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

M. Mitchell

Verified customer

Asked: 2013-10-29

Answer

The products we sell, including anti-CD30 / TNFRSF8 (Hodgkin & Reed-Sternberg Cell Marker) Monoclonal antibody M01225, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2013-10-29

Question

Will anti-CD30 / TNFRSF8 (Hodgkin & Reed-Sternberg Cell Marker) Monoclonal antibody M01225 work on primate IHC with blood testis?

C. Jones

Verified customer

Asked: 2013-08-01

Answer

Our lab technicians have not validated anti-CD30 / TNFRSF8 (Hodgkin & Reed-Sternberg Cell Marker) Monoclonal antibody M01225 on primate. You can run a BLAST between primate and the immunogen sequence of anti-CD30 / TNFRSF8 (Hodgkin & Reed-Sternberg Cell Marker) Monoclonal antibody M01225 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate blood testis in IHC, you can get your next antibody for free.

Boster Scientific Support

Answered: 2013-08-01

Size

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Ships directly to USA and Canada. For other regions, visit our distributors page

Bulk Inquiry

In stock
Order Product
M01225
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$399.00

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