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SKU:PA1334
Clonality:Polyclonal
Application:IHC-P, IHC-F, WB
Price: $200.00
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Overview

Product Name Anti-CD34 Antibody
Description Rabbit IgG polyclonal antibody for Hematopoietic progenitor cell antigen CD34(CD34) detection. Tested with WB, IHC-P, IHC-F in Human;Mouse;Rat.
Cite This Product Anti-CD34 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1334)
Replacement Item This antibody may replace the following items: sc-9095|sc-133082|sc-7045|sc-19621|sc-74499|sc-65261|sc-18917|sc-52478|sc-53511|sc-19587|sc-52312|sc-7324 from Santa Cruz Biotechnology.
Host Rabbit
Isotype N/A
Validated Species Human, Mouse, Rat
Predicted Species Hamster

*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.

Application IHC-P, IHC-F, WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P) and IHC(F).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen A synthetic peptide corresponding to a sequence at the C-terminus of human CD34(366-382aa QATSRNGHSARQHVVAD), identical to the related mouse and rat sequences.
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Polyclonal
Form Lyophilized
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
Concentration Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Immunogen affinity purified.
Isotype N/A

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name CD34
Protein Name Hematopoietic progenitor cell antigen CD34
Molecular Weight 40716 MW
Protein Function Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins.
Tissue Specificity Selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues.
Sequence Similarities Belongs to the CD34 family.
Subcellular Localization Membrane; Single-pass type I membrane protein.
Uniprot ID P28906
Alternative Names Hematopoietic progenitor cell antigen CD34;CD34;CD34;
Research Areas |neuroscience|cell type marker|neuron marker|soma marker| immunology|cell type markers|myeloid cells| stem cells|lineage markers|mesoderm|mesenchymal stem cells|negative markers|hematopoietic progenitors|surface molecules| cancer|tumor biomarkers| cardiovascular|heart|cardiogenesis|hematopoietic stem cells|hsc markers|endothelial progenitors|endothelial markers| developmental biology|lineage specification|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Hematopoietic progenitor cell antigen CD34

CD34 is a monomeric cell surface antigen with a molecular mass of approximately 110 KD.CD34 is expressed in humans in hematopoietic stem cells, vascular endothelium, and blasts from 30% of patients with acute myeloid and lymphocytic leukemia. The human CD34 gene spans 26 kb and has 8 exons, a structure quite similar to that of the murine gene. By Southern blot analysis of DNA from a panel of human x mouse somatic cell hybrids using a CD34 cDNA probe demonstrate that the gene for CD34 is located on human chromosome 1 in the 1q12----qter region. CD34 plays an important role in the formation of progenitor cells during both embryonic and adult hematopoiesis.

Anti-CD34 Antibody Images

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Anti-CD34 Antibody
Anti-CD34 antibody, PA1334, Western blotting
Lane 1: Recombinant Human CD34 Protein 10ng
Lane 2: Recombinant Human CD34 Protein 5ng
Lane 3: Recombinant Human CD34 Protein 2.5ng
Anti-CD34 Antibody
Anti-CD34 antibody, PA1334, IHC(P)
IHC(P): Rat Kidney Tissue
Anti-CD34 Antibody
Anti-CD34 antibody, PA1334, IHC(P)
IHC(P): Human Lung Cancer Tissue
Anti-CD34 Antibody
Anti-CD34 antibody, PA1334, IHC(F)
IHC(F): Rat Kidney Tissue
Anti-CD34 Antibody
Anti-CD34 antibody, PA1334, IHC(F)
IHC(F): Rat Placenta Tissue
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Publications

Li D, Wei X, Xie K, Chen K, Li J, Fang J. Br J Cancer. 2014 Jul 8;111(1):68-77. Doi: 10.1038/Bjc.2014.282. Epub 2014 May 29. A Novel Decoy Receptor Fusion Protein For Fgf-2 Potently Inhibits Tumour Growth.
Niu Yc, Liu Jc, Zhao Xm, Wu Xx. Oncol Rep. 2009 Jan;21(1):145-52. A Low Molecular Weight Polysaccharide Isolated From Agaricus Blazei Suppresses Tumor Growth And Angiogenesis In Vivo.
Wang Q, Zhu J, Zou L, Yang Y. J Huazhong Univ Sci Technolog Med Sci. 2011 Apr;31(2):246-50. Doi: 10.1007/S11596-011-0261-2. Epub 2011 Apr 20. Role Of Axonal Guidance Factor Netrin-1 In Human Placental Vascular Growth.
Chen Y, Chen L, Li Jy, Mukaida N, Wang Q, Yang C, Yin Wj, Zeng Xh, Jin W, Shao Zm. Cancer Biol Ther. 2011 Mar 1;11(5):497-511. Epub 2011 Mar 1. Er?? And Pea3 Co-Activate Il-8 Expression And Promote The Invasion Of Breast Cancer Cells.
Liu Hp, Gao Zh, Cui Sx, Sun Df, Wang Y, Zhao Cr, Lou Hx, Qu Xj. Plos One. 2012;7(3):E33243. Doi: 10.1371/Journal.Pone.0033243. Epub 2012 Mar 14. Inhibition Of Intestinal Adenoma Formation In Apc(Min/+) Mice By Riccardin D, A Natural Product Derive...
Jie Li, Lu Wm, Li Xx, Wang Sm, Yu Jx, Zhu Yf, Liu Dy, Huang Mq. Angiology. 2010 Jul;61(5):427-36. Doi: 10.1177/0003319709356422. Epub 2010 Apr 14. Intensive Statin Therapy: A Favorable Adjunct To The Improvement Of Small-Diameter Vascular Grafts.
Zhang Qq, Ding Y, Lei Y, Qi Cl, He Xd, Lan T, Li Jc, Gong P, Yang X, Geng Jg, Wang Lj. Int J Biol Sci. 2014 Mar 21;10(4):404-14. Doi: 10.7150/Ijbs.7723. Ecollection 2014. Andrographolide Suppress Tumor Growth By Inhibiting Tlr4/Nf-??b Signaling Act...
Luo Bh, Xiong F, Wang Jp, Li Jh, Zhong M, Liu Ql, Luo Gq, Yang Xj, Xiao N, Xie B, Xiao H, Liu Rj, Dong Cs, Wang Ks, Wen Jf. Plos One. 2014 Jun 19;9(6):E99922. Doi: 10.1371/Journal.Pone.0099922. Ecollection 2014. Epidermal Growth Factor-Like Domain...
Sun D, Bu L, Liu C, Yin Z, Zhou X, Li X, Xiao A. Plos One. 2013 May 28;8(5):E65042. Doi: 10.1371/Journal.Pone.0065042. Print 2013. Therapeutic Effects Of Human Amniotic Fluid-Derived Stem Cells On Renal Interstitial Fibrosis In A Murine Model Of U...
Lin H, Liu F, Zhang C, Zhang Z, Guo J, Ren C, Kong Z. Mol Neurobiol. 2009 Dec;40(3):216-23. Doi: 10.1007/S12035-009-8082-Z. Epub 2009 Sep 2. Pluripotent Hair Follicle Neural Crest Stem-Cell-Derived Neurons And Schwann Cells Functionally Repair Sci...
Lin H, Liu F, Zhang C, Zhang Z, Kong Z, Zhang X, Hoffman Rm. Tissue Eng Part A. 2011 Jul;17(13-14):1691-8. Doi: 10.1089/Ten.Tea.2010.0514. Epub 2011 Apr 8. Characterization Of Nerve Conduits Seeded With Neurons And Schwann Cells Derived From Hair ...
Pan X, Zhu Q, Sun Y, Li L, Zhu Y, Zhao Z, Zuo J, Fang W, Li K. Int J Mol Med. 2015 Apr;35(4):995-1002. Doi: 10.3892/Ijmm.2015.2096. Epub 2015 Feb 11. Plga/Poloxamer Nanoparticles Loaded With Epas1 Sirna For The Treatment Of Pancreatic Cancer In Vi...
Fu W, Lv C, Zhuang W, Chen D, Lv E, Li F, Wang X. Neural Regen Res. 2013 Feb 15;8(5):427-34. Doi: 10.3969/J.Issn.1673-5374.2013.05.006. An Effective Inducer Of Dopaminergic Neuron-Like Differentiation.
Qiu W, Lei M, Li J, Wang N, Lian X. Int J Med Sci. 2014 Jul 30;11(10):1022-8. Doi: 10.7150/Ijms.8383. Ecollection 2014. Activated Hair Follicle Stem Cells And Wnt/??-Catenin Signaling Involve In Pathnogenesis Of Sebaceous Neoplasms.
Zhang J, Yang W, Zhao D, Han Y, Liu B, Zhao H, Wang H, Zhang Q, Xu G. Oncol Lett. 2014 Jan;7(1):95-100. Epub 2013 Oct 29. Correlation Between Tsp-1, Tgf-?? And Ppar-?? Expression Levels And Glioma Microvascular Density.
Zhang J, Wang Y, Zhao B, Fan L, Bai X, Yang L, Chang P, Hu D, Liu X. Aesthetic Plast Surg. 2015 Aug;39(4):625-34. Doi: 10.1007/S00266-015-0505-9. Epub 2015 Jun 5. Allogeneic Adipose-Derived Stem Cells Protect Fat Grafts At The Early Stage And Impr...
Lin Q, Ding X, Qiu F, Song X, Fu G, Ji J. Biomaterials. 2010 May;31(14):4017-25. Doi: 10.1016/J.Biomaterials.2010.01.092. Epub 2010 Feb 9. In Situ Endothelialization Of Intravascular Stents Coated With An Anti-Cd34 Antibody Functionalized Heparin-...
Chen W, Lu Y, Wu J, Gao M, Wang A, Xu B. Cancer Chemother Pharmacol. 2011 Apr;67(4):799-808. Doi: 10.1007/S00280-010-1378-X. Epub 2010 Jun 19. Beta-Elemene Inhibits Melanoma Growth And Metastasis Via Suppressing Vascular Endothelial Growth Factor-...
Zeng R, Chen Yc, Zeng Z, Liu Wq, Jiang Xf, Liu R, Qiang O, Li X. Heart Vessels. 2011 Jan;26(1):69-80. Doi: 10.1007/S00380-010-0032-1. Epub 2010 Oct 21. Effect Of Mini-Tyrosyl-Trna Synthetase/Mini-Tryptophanyl-Trna Synthetase On Ischemic Angiogenes...
Chen W, Lu Y, Gao M, Wu J, Wang A, Shi R. J Ethnopharmacol. 2011 Jan 7;133(1):220-6. Doi: 10.1016/J.Jep.2010.09.031. Anti-Angiogenesis Effect Of Essential Oil From Curcuma Zedoaria In Vitro And In Vivo.
Yu Y, Gao S, Li Q, Wang C, Lai X, Chen X, Wang R, Di J, Li T, Wang W, Wu X. J Cancer Res Clin Oncol. 2012 Aug;138(8):1321-8. Doi: 10.1007/S00432-012-1201-7. Epub 2012 Apr 6. The Fgf2-Binding Peptide P7 Inhibits Melanoma Growth In Vitro And In Vivo.
Hu Y, Chen L, Yi C, Yang F, Chen J. J Huazhong Univ Sci Technolog Med Sci. 2012 Aug;32(4):581-5. Doi: 10.1007/S11596-012-1000-Z. Epub 2012 Aug 11. Experimental Study On Inhibitory Effects Of Diallyl Sulfide On Growth And Invasion Of Human Osteosar...
Pan B, Yu B, Ren H, Willard B, Pan L, Zu L, Shen X, Ma Y, Li X, Niu C, Kong J, Kang S, Eugene Chen Y, Pennathur S, Zheng L. Free Radic Biol Med. 2013 Jul;60:272-81. Doi: 10.1016/J.Freeradbiomed.2013.02.004. Epub 2013 Feb 14. High-Density Lipoprote...
Li Lx, Zhang Yl, Zhou L, Ke Ml, Chen Jm, Fu X, Ye Cl, Wu Jx, Liu Ry, Huang W. J Transl Med. 2013 Oct 14;11:257. Doi: 10.1186/1479-5876-11-257. Antitumor Efficacy Of A Recombinant Adenovirus Encoding Endostatin Combined With An E1B55Kd-Deficient Ad...
Yang Z, Yang Y, Yan W, Tu Q, Wang J, Huang N. Acs Appl Mater Interfaces. 2013 Nov 13;5(21):10495-501. Doi: 10.1021/Am403478K. Epub 2013 Oct 25. Construction Of Polyfunctional Coatings Assisted By Gallic Acid To Facilitate Co-Immobilization Of Dive...
Yang J, Xiong Lj, Xu F, Zhao X, Liu B, Cai Kl, Wang Gb. Int J Endocrinol. 2013;2013:453898. Doi: 10.1155/2013/453898. Epub 2013 Nov 14. Estrogen Inhibits Colon Polyp Formation By Reducing Angiogenesis In A Carcinogen-Induced Rat Model.
Wang G, Xu D, Chai Q, Tan X, Zhang Y, Gu N, Tang J. Oncol Lett. 2014 May;7(5):1370-1374. Epub 2014 Feb 20. Magnetic Fluid Hyperthermia Inhibits The Growth Of Breast Carcinoma And Downregulates Vascular Endothelial Growth Factor Expression.
Zhu Sk, Zhou Y, Cheng C, Zhong S, Wu Hq, Wang B, Fan P, Xiong Jx, Yang Hj, Wu Hs. Mol Clin Oncol. 2014 Nov;2(6):973-981. Epub 2014 Jul 9. Overexpression Of Membrane-Type 2 Matrix Metalloproteinase Induced By Hypoxia-Inducible Factor-1?? In Pancreat...
Zhang Qq, Zhou Dl, Ding Y, Liu Hy, Lei Y, Fang Hy, Gu Ql, He Xd, Qi Cl, Yang Y, Lan T, Li Jc, Gong P, Wu Xy, Yang X, Li Wd, Wang Lj. Melanoma Res. 2014 Dec;24(6):545-55. Doi: 10.1097/Cmr.0000000000000117. Andrographolide Inhibits Melanoma Tumor Gr...
Yi W, Xu Ht, Tian Df, Wu Lq, Zhang Sq, Wang L, Ji Bw, Zhu Xn, Okechi H, Liu G, Chen Qx. J Huazhong Univ Sci Technolog Med Sci. 2015 Apr;35(2):259-64. Doi: 10.1007/S11596-015-1421-6. Epub 2015 Apr 16. Photodynamic Therapy Mediated By 5-Aminolevulin...
Jing Z, Shi C, Zhu L, Xiang Y, Chen P, Xiong Z, Li W, Ruan Y, Huang L. J Cereb Blood Flow Metab. 2015 Aug;35(8):1249-59. Doi: 10.1038/Jcbfm.2015.55. Epub 2015 Apr 8. Chronic Cerebral Hypoperfusion Induces Vascular Plasticity And Hemodynamics But A...
Li J, Ye Y, Zhang R, Zhang L, Hu X, Han D, Chen J, He X, Wang G, Yang X, Wang L. Sci Rep. 2015 May 19;5:9720. Doi: 10.1038/Srep09720. Robo1/2 Regulate Follicle Atresia Through Manipulating Granulosa Cell Apoptosis In Mice.
Wang Q, Zhong Yj, Yuan Jp, Shao Lh, Zhang J, Tang L, Liu Sp, Hong Yp, Firestone Ra, Li Y. J Transl Med. 2013 Aug 21;11:192. Doi: 10.1186/1479-5876-11-192. Targeting Therapy Of Hepatocellular Carcinoma With Doxorubicin Prodrug Pdox Increases Anti-M...
Chen J, Jia Zy, Ma Zl, Wang Yy, Teng Gj. Plos One. 2011;6(6):E20790. Doi: 10.1371/Journal.Pone.0020790. Epub 2011 Jun 24. In Vivo Serial Mr Imaging Of Magnetically Labeled Endothelial Progenitor Cells Homing To The Endothelium Injured Artery In Mice.
Huang Y, Jia X, Bai K, Gong X, Fan Y. Arch Med Res. 2010 Oct;41(7):497-505. Doi: 10.1016/J.Arcmed.2010.10.002. Effect Of Fluid Shear Stress On Cardiomyogenic Differentiation Of Rat Bone Marrow Mesenchymal Stem Cells.
Liu D, Wang Y, Ye Y, Yin G, Chen L. Cell Immunol. 2014 May-Jun;289(1-2):7-14. Doi: 10.1016/J.Cellimm.2014.01.007. Epub 2014 Feb 2. Distinct Molecular Basis For Endothelial Differentiation: Gene Expression Profiles Of Human Mesenchymal Stem Cells V...
Tochinai R, Ando M, Suzuki T, Suzuki K, Nagata Y, Hata C, Uchida K, Kobayashi T, Kado S, Kaneko K. Exp Toxicol Pathol. 2013 Sep;65(6):737-43. Doi: 10.1016/J.Etp.2012.09.008. Epub 2012 Nov 4. Histopathological Studies Of Microtubule Disassembling A...
Shan X, Wang D, Chen J, Xiao X, Jiang Y, Wang Y, Fan Y. J Comput Assist Tomogr. 2013 Jan-Feb;37(1):22-8. Doi: 10.1097/Rct.0B013E318279Abd1. Necrosis Degree Displayed In Computed Tomography Images Correlated With Hypoxia And Angiogenesis In Breast ...
Zhou J, Wang L, Xu X, Tu Y, Qin S, Yin Y. Oncol Lett. 2012 Nov;4(5):976-980. Epub 2012 Aug 8. Antitumor Activity Of Endostar Combined With Radiation Against Human Nasopharyngeal Carcinoma In Mouse Xenograft Models.
Kong J, Kong J, Pan B, Ke S, Dong S, Li X, Zhou A, Zheng L, Sun Wb. Plos One. 2012;7(5):E37266. Doi: 10.1371/Journal.Pone.0037266. Epub 2012 May 15. Insufficient Radiofrequency Ablation Promotes Angiogenesis Of Residual Hepatocellular Carcinoma Vi...
Li Hm, Liu L, Mei X, Chen H, Liu Z, Zhao X. Life Sci. 2014 Jun 13;106(1-2):50-7. Doi: 10.1016/J.Lfs.2014.04.020. Epub 2014 Apr 26. Overexpression Of Inducible Nitric Oxide Synthase Impairs The Survival Of Bone Marrow Stem Cells Transplanted Into R...
Zhang G, Lan X, Yen Tc, Chen Q, Pei Z, Qin C, Zhang Y. Nucl Med Biol. 2012 Nov;39(8):1243-50. Doi: 10.1016/J.Nucmedbio.2012.06.010. Epub 2012 Jul 15. Therapeutic Gene Expression In Transduced Mesenchymal Stem Cells Can Be Monitored Using A Reporte...
Huang Xf, Yuan Sj, Yang C. Mol Med Rep. 2012 Sep;6(3):547-52. Doi: 10.3892/Mmr.2012.974. Epub 2012 Jun 29. Effects Of Total Flavonoids From Drynaria??fortunei On The Proliferation And Osteogenic Differentiation Of Rat Dental Pulp Stem C...
Du G, Liu Y, Dang M, Zhu G, Su R, Fan Y, Tan Z, Wang Lx, Fang J. Acta Histochem. 2014 Jul;116(6):1075-84. Doi: 10.1016/J.Acthis.2014.05.002. Epub 2014 Jun 21. Comparison Of Administration Routes For Adipose-Derived Stem Cells In The Treatment Of M...
Liu T, Liu Y, Chen Y, Liu S, Maitz Mf, Wang X, Zhang K, Wang J, Wang Y, Chen J, Huang N. Acta Biomater. 2014 May;10(5):1940-54. Doi: 10.1016/J.Actbio.2013.12.013. Epub 2013 Dec 14. Immobilization Of Heparin/Poly-(L)-Lysine Nanoparticles On Dopamin...
Luo Q, Liu X, Zheng Y, Zhao Y, Zhu J, Zou L. Placenta. 2015 May;36(5):567-74. Doi: 10.1016/J.Placenta.2015.02.009. Epub 2015 Feb 21. Ephrin-B2 Mediates Trophoblast-Dependent Maternal Spiral Artery Remodeling In First Trimester.
Ren Y, Wu H, Zhou X, Wen J, Jin M, Cang M, Guo X, Wang Q, Liu D, Ma Y. Res Vet Sci. 2012 Aug;93(1):404-11. Doi: 10.1016/J.Rvsc.2011.08.014. Epub 2011 Sep 25. Isolation, Expansion, And Differentiation Of Goat Adipose-Derived Stem Cells.
Li Ql, Huang N, Chen C, Chen Jl, Xiong Kq, Chen Jy, You Tx, Jin J, Liang X. J Biomed Mater Res A. 2010 Sep 15;94(4):1283-93. Doi: 10.1002/Jbm.A.32812. Oriented Immobilization Of Anti-Cd34 Antibody On Titanium Surface For Self-Endothelialization In...
Sun Y, Chen S, Song G, Ren L, Wei L, Liu N, Zhang D, Lv X. Int J Mol Med. 2012 Sep;30(3):622-8. Doi: 10.3892/Ijmm.2012.1032. Epub 2012 Jun 14. Effect Of Visfatin On The Function Of Endothelial Progenitor Cells In High-Fat-Fed Obese Rat...
Song D, Yue W, Li Z, Li J, Zhao J, Zhang N. Onco Targets Ther. 2014 Sep 30;7:1801-10. Doi: 10.2147/Ott.S52426. Ecollection 2014. Study Of The Mechanism Of Sonodynamic Therapy In A Rat Glioma Model.
Ge Y, Ding Y, Zhang J, Li Z, Li Z. Int J Clin Exp Med. 2014 Oct 15;7(10):3578-82. Ecollection 2014. Effect Of Angiogenesis Inhibitor Su6668 In Combination With 5-Fu On Liver Metastasis From Transplantation Tumors Of Human Colorectal Cancer In Nude...
Zhou L, Zhang K, Li J, Cui X, Wang A, Huang S, Zheng S, Lu Y, Chen W. Reprod Toxicol. 2013 Jun;37:62-9. Doi: 10.1016/J.Reprotox.2013.02.001. Epub 2013 Feb 11. Inhibition Of Vascular Endothelial Growth Factor-Mediated Angiogenesis Involved In Repro...
Jiang Jt, Zhang Lf, Zhou B, Zhang Sq, Li Sm, Zhang W, Zhang J, Qiao Z, Kong Rr, Ma Yf, Chen S. Asian Pac J Cancer Prev. 2012;13(7):3379-83. Relationships Of Upa And Vegf Expression In Esophageal Cancer And Microvascular Density With Tumorous Invas...
Jiang Z, Han B, Li H, Yang Y, Liu W. Carbohydr Polym. 2015 Sep 20;129:1-8. Doi: 10.1016/J.Carbpol.2015.04.040. Epub 2015 Apr 27. Carboxymethyl Chitosan Represses Tumor Angiogenesis In Vitro And In Vivo.

FAQs

Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: I performed a 1:500 dilution for the working solution of this antibody (500 µg/mL). The immunostaining signal looks weak. Please advice for the next step.
A: In this case, try using a 1:200 dilution to see if the signal looks stronger.
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
A: You can find the immunogen sequence under "Immunogen" and clonality in the product name.
Q: What is the expected band size? Why is it different than the observed band size?
A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands

3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.

4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
A: Check our protocols under the tech support tab.