|Application:||Flow Cytometry, IHC-P, IHC-F, ICC, WB|
Data & Images
|Product Name||Anti-CD34 Picoband™ Antibody|
|Description||Rabbit IgG polyclonal antibody for Hematopoietic progenitor cell antigen CD34(CD34) detection. Tested with WB, IHC-P, IHC-F, ICC, FCM in Human;Mouse;Rat.|
|Cite This Product||Anti-CD34 Picoband™ Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9053)|
|Replacement Item||This antibody may replace the following items: sc-9095|sc-133082|sc-7045|sc-19621|sc-74499|sc-65261|sc-18917|sc-52478|sc-53511|sc-19587|sc-52312|sc-7324 from Santa Cruz Biotechnology.|
|Validated Species||Human, Mouse, Rat|
|Application||Flow Cytometry, IHC-P, IHC-F, ICC, WB
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P), IHC(F) and ICC.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||E.coli-derived human CD34 recombinant protein (Position: T151-L385). Human CD34 shares 79% amino acid (aa) sequence identity with mouse CD34.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
*carrier free antibody available upon request.
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Hematopoietic progenitor cell antigen CD34|
|Molecular Weight||40716 MW|
|Protein Function||Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins.|
|Tissue Specificity||Selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues.|
|Sequence Similarities||Belongs to the CD34 family.|
|Subcellular Localization||Membrane; Single-pass type I membrane protein.|
|Alternative Names||Hematopoietic progenitor cell antigen CD34;CD34;CD34;|
|Research Areas|||neuroscience|cell type marker|neuron marker|soma marker| immunology|cell type markers|myeloid cells| stem cells|lineage markers|mesoderm|mesenchymal stem cells|negative markers|hematopoietic progenitors|surface molecules| cancer|tumor biomarkers| cardiovascular|heart|cardiogenesis|hematopoietic stem cells|hsc markers|endothelial progenitors|endothelial markers| developmental biology|lineage specification||
Background for Hematopoietic progenitor cell antigen CD34
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-CD34 Picoband™ Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, By Heat|
Immunohistochemistry(Frozen Section), 0.5-1μg/ml
Western blot, 0.1-0.5μg/ml
Flow Cytometry, 1-3μg/1x106cells
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-CD34 Picoband™ Antibody Images
Click the images to enlarge.
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: MCF-7 Whole Cell Lysate
Lane 2: MM231 Whole Cell Lysate
Lane 3: SMMC Whole Cell Lysate
Lane 4: HepG2 Whole Cell Lysate
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD34 antigen affinity purified polyclonal antibody (Catalog # PB9053) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD34 at approximately 41KD. The expected band size for CD34 is at 41KD.
CD34 was detected in paraffin-embedded section of Human Lung Cancer Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CD34 Antibody (PB9053) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Overlay histogram showing Raji cells stained with PB9053 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CD34 Antibody (PB9053,1μg/1x106 cells) for 30 min at 20°C. DyLight?488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
"[Boster anti-CD34] demonstrated positive staining of Jurkat cells"
--Antibodyresource validation lab
"Under these experimental conditions... CD34 P35 [Boster anti-CD14] demonstrated positive staining of Jurkat cells whilst for all the other tested antibodies negative staining was observed."
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,