|Applications||Flow Cytometry, IF, IHC, ICC, WB|
|Product Name||Anti-CD4 Rabbit Monoclonal Antibody|
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.|
|Description||Anti-CD4 Rabbit Monoclonal Antibodytested for Flow Cytometry, IF, IHC, ICC, WB in Human|
|Cite This Product||Anti-CD4 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00344)|
|Immunogen||A synthesized peptide derived from human CD4|
Assay Dilutions Overview
Flow Cytometry 1:50
Images And Assay Conditions
Western blot analysis of CD4 expression in THP-1 cell lysate (M00344).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD4 monoclonal antibody (Catalog # M00344) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD4
Protein Target Info (Source: Uniprot.org)
|Protein Name||T-cell surface glycoprotein CD4|
|Alternative Names||T-cell surface glycoprotein CD4;T-cell differentiation antigen L3T4;T-cell surface antigen T4/Leu-3;CD4;Cd4;|
|Subcellular Localization||Membrane; Single-pass type I membrane protein.|
|Molecular Weight||51297 MW|
*if product is indicated to react with multiple species, protein info is based on the human gene.
|Protein Function||Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation.|
Order Product (M00344)
Promotion:Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
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