SKU M00344
Size 100ug/vial
Reactivity Human
Clonality Monoclonal
Clone Number DOE-3
Host Rabbit
Applications IF, WB

Overview

Product Name Anti-CD4 Rabbit Monoclonal Antibody
SKU/Catalog Number M00344
Storage & Handling At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
Size 100ug/vial
Description Anti-CD4 Rabbit Monoclonal Antibodytested for Flow Cytometry, IF, IHC, ICC, WB in Human
Cite This Product Anti-CD4 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00344)
Host Rabbit
Contents/Buffer Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen A synthesized peptide derived from human CD4
Reactivity Human

Assay Details

Assay Dilutions Overview

Concentration: 0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
Flow Cytometry 1:50

Images And Assay Conditions

/M/0/M00344 CD4 primary antibodies WB testing 1.jpg

Western blot analysis of CD4 expression in THP-1 cell lysate (M00344).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD4 monoclonal antibody (Catalog # M00344) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD4

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id P01730
Gene Name CD4
Protein Name T-cell surface glycoprotein CD4
Alternative Names T-cell surface glycoprotein CD4;T-cell differentiation antigen L3T4;T-cell surface antigen T4/Leu-3;CD4;Cd4;
Subcellular Localization Membrane; Single-pass type I membrane protein.
Molecular Weight 51297 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation.
Research Areas Adaptive Immunity, Antiviral Signaling, Hematopoietic Progenitors, Immune System Diseases, Immunology, Lymphoid, Myeloid, Stem Cells, T Cells, T Lymphocytic Lineage

*You can search these to find other products in these research areas.

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Order Product (M00344)

Promotion:

Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
Option Price
100ug $299
100ug+Free HRP Secondary BA1054 $299
100ug+Free Biotin Secondary BA1003 $299
30ug sample size $99

USD $299

Ships in 7-10 business days.

Troubleshooting

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Publications

Elevated survivin expression in peripheral blood mononuclear cells is central to collateral formation in coronary chronic total occlusion.
Effect of Hejie decoction on T cell immune state of chronic hepatitis B patients