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SKU:PA1019-1
Clonality:Polyclonal
Application:WB
Price: $200.00
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Overview

Product Name Anti-CD40/TNFRSF5 Antibody
Description Rabbit IgG polyclonal antibody for Tumor necrosis factor receptor superfamily member 5(CD40) detection. Tested with WB in Human.
Cite This Product Anti-CD40/TNFRSF5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1019-1)
Replacement Item This antibody may replace the following items: sc-30685|sc-1731|sc-30681|sc-974|sc-13528|sc-9096|sc-13128|sc-30683|sc-65264|sc-975|sc-65263|sc-30684|sc-20010|sc-59047|sc-514493 from Santa Cruz Biotechnology.
Host Rabbit
Isotype N/A
Validated Species Human
Application WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen A synthetic peptide corresponding to a sequence at the N-terminus of human CD40(20-37aa, PEPPTACREKQYLINSQC).
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Polyclonal
Form Lyophilized
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
Concentration Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Immunogen affinity purified.
Isotype N/A

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name CD40
Protein Name Tumor necrosis factor receptor superfamily member 5
Molecular Weight 30619 MW
Protein Function Receptor for TNFSF5/CD40LG. Transduces TRAF6- and MAP3K8-mediated signals that activate ERK in macrophages and B cells, leading to induction of immunoglobulin secretion.
Tissue Specificity B-cells and in primary carcinomas.
Sequence Similarities Contains 4 TNFR-Cys repeats.
Subcellular Localization Isoform I: Cell membrane; Single-pass type I membrane protein.
Uniprot ID P25942
Alternative Names Tumor necrosis factor receptor superfamily member 5;B-cell surface antigen CD40;Bp50;CD40L receptor;CDw40;CD40;CD40;TNFRSF5;
Research Areas |immunology|adaptive immunity|b cells| cardiovascular|atherosclerosis|vascular inflammation|innate and adaptive immunity| stem cells|hematopoietic progenitors|lymphoid|b lymphocytic lineage|myeloid|dendritic cell lineage|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Tumor necrosis factor receptor superfamily member 5

CD40 is a cell surface receptor that is expressed on the surface of all mature B cells and also expressed on monocytes, dendritic cells, and thymic epithelium. CD40 is a member of the tumor necrosis factor(TNF) receptor superfamily and is the receptor for CD40 ligand. CD40 ligand(CD40L, CD154, gp39, and TRAM) belongs to the TNF gene family and is expressed more widely than CD40 predominantly on activated CD4+ T cells. Activation of CD40 has also been shown to inhibit the growth of certain B cell lymphomas and to induce the death of transformed cells of mesenchymal or epithelial origin.

Anti-CD40/TNFRSF5 Antibody Images

Click the images to enlarge.

Anti-CD40/TNFRSF5 Antibody
Anti-CD40 antibody, PA1019-1, Western blotting
Lane 1: Recombinant Human CD40 Protein 10ng
Lane 2: Recombinant Human CD40 Protein 5ng
Lane 3: Recombinant Human CD40 Protein 2.5ng
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Publications

Meng Hx, Li Hn, Geng Js, Ohe R, Yu Xy, E Xq, Ye F, Yang Sr, Kato T, Zhang L, Ishida A, Ohta N, Jin Xm, Kakehata S, Geng Js, Yamakawa M. Transl Res. 2015 Sep;166(3):281-91. Doi: 10.1016/J.Trsl.2015.04.004. Epub 2015 Apr 17. Decreased Expression Of ...
Peng Yd, Cheng Lx, Zeng Qt, Shi Y, Bao Xx, Mao Xb, Ji Qw, Li Sn, Guo M, Liang Zs, Wu Tc. Cell Biol Int. 2010 Mar 24;34(5):447-53. Doi: 10.1042/Cbi20090301. The Role Of Rantes As A Crucial Downstream Cytokine In Calcineurin-Dependent Vsmc Apoptosis...

FAQs

Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: How come some of your antibodies have more than one catalog numbers for the same target? One example is anti-CD40 antibody which has two catalog numbers: PA1019, PA1019-1.
A: It is because for some targets we have at least two antibodies with different immunogen sequences to target different epitopes and/or species. For the case of anti-CD40 antibody, PA1019 has an immunogen N-terminus of mouse CD40 (22-36 aa, QCVTCSDKQYLHDGQ), while the immunogen for PA1019-1 is N-terminus of human CD40 (20-37 aa, PEPPTACREKQYLINSQC).
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
A: You can find the immunogen sequence under "Immunogen" and clonality in the product name.
Q: What is the expected band size? Why is it different than the observed band size?
A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands

3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.

4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
A: Check our protocols under the tech support tab.