|Application:||IHC-P, ICC, WB|
Data & Images
|Product Name||Anti-CD43 Picoband™ Antibody|
|Description||Rabbit IgG polyclonal antibody for Leukosialin(SPN) detection. Tested with WB, IHC-P, ICC in Human;Mouse;Rat.|
|Cite This Product||Anti-CD43 Picoband™ Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9095)|
|Replacement Item||This antibody may replace the following items: sc-14773|sc-14774|sc-20819|sc-21774|sc-32933|sc-373974|sc-376477|sc-398387|sc-514180|sc-51727|sc-6256|sc-69012|sc-69013|sc-69014|sc-7052|sc-7053|sc-70681|sc-70682 from Santa Cruz Biotechnology.|
|Validated Species||Human, Mouse, Rat|
|Application||IHC-P, ICC, WB
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P) and ICC.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||E.coli-derived human CD43 recombinant protein (Position: A272-P400). Human CD43 shares 72% and 73% amino acid (aa) sequences identity with mouse and rat CD43, respectively.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
*carrier free antibody available upon request.
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Molecular Weight||40322 MW|
|Protein Function||One of the major glycoproteins of thymocytes and T lymphocytes. Plays a role in the physicochemical properties of the T-cell surface and in lectin binding. Presents carbohydrate ligands to selectins. Has an extended rodlike structure that could protrude above the glycocalyx of the cell and allow multiple glycan chains to be accessible for binding. Is a counter-receptor for SN/Siglec-1 (By similarity). During T-cell activation is actively removed from the T-cell-APC (antigen-presenting cell) contact site thus suggesting a negative regulatory role in adaptive immune response (By similarity). .|
|Tissue Specificity||Cell surface of thymocytes, T-lymphocytes, neutrophils, plasma cells and myelomas.|
|Subcellular Localization||Membrane; Single-pass type I membrane protein.|
|Alternative Names||Leukosialin;Galactoglycoprotein;GALGP;Leukocyte sialoglycoprotein;Sialophorin;CD43;SPN;CD43;|
|Research Areas|||cell biology|apoptosis|intracellular|bcl2 family||
Background for Leukosialin
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-CD43 Picoband™ Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunocytochemistry , 0.5-1μg/ml, Human, -|
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Western blot, 0.1-0.5μg/ml, Human
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-CD43 Picoband™ Antibody Images
Click the images to enlarge.
All lanes: Anti CD43 (PB9095) at 0.5ug/ml
WB: Recombinant Human CD43 Protein 0.5ng
Predicted bind size: 47KD
Observed bind size: 47KD
IHC(P): Mouse Spleen Tissue
IHC(P): Rat Spleen Tissue
IHC(P): Human Tonsil Tissue
ICC: K562 Cell
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,