Anti-CD44 antigen CD44 Antibody

CD44 antibody

Boster Bio Anti-CD44 antigen CD44 Antibody catalog # PA1021-2. Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Rat. Cited in 10 publication(s).

Product Info Summary

SKU: PA1021-2
Size: 100 μg/vial
Reactive Species: Human, Rat
Host: Rabbit
Application: Flow Cytometry, IHC, WB

Product Name

Anti-CD44 antigen CD44 Antibody

View all CD44 Antibodies

SKU/Catalog Number

PA1021-2

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-CD44 antigen CD44 Antibody catalog # PA1021-2. Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-CD44 antigen CD44 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1021-2)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human CD44, different from the related mouse and rat sequences by one amino acid.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1021-2 is reactive to CD44 in Human, Rat

Applications

PA1021-2 is guaranteed for Flow Cytometry, IHC, WB Boster Guarantee

Observed Molecular Weight

82 kDa

Calculated molecular weight

81.538kDa

Background of CD44

The CD44 gene, which is a transmembrane protein, is expressed as a family of molecular isoforms generated from alternative RNA splicing and posttranslational modifications. The gene, which contains 19 exons spanning some 50 kb of genomic DNA, is a widely expressed integral membrane protein that acts as a receptor for hyaluronan (HA) and is important to cell-extracellular matrix interaction. CD44 binding with HA can play an important role in cellular aggregation and tumor cell growth. CD44 is necessary for limb development and functions in a novel growth factor presentation mechanism. A specific CD44 splice variant is crucial for the proliferation of these mesenchymal cells. CD44 glycoproteins are involved in leukocyte extravasation but also in the regulation of growth factor activation, stability, and signaling. Moreover, it plays a pivotal role in arteriogenesis.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human, Rat, By Heat
Flow Cytometry, 1-3 μg/1x106 cells, Human

Validation Images & Assay Conditions

Gene/Protein Information For CD44 (Source: Uniprot.org, NCBI)

Gene Name

CD44

Full Name

CD44 antigen

Weight

81.538kDa

Alternative Names

CD44 antigen; CD44 molecule (Indian blood group); CD44; CD44R; CDw44; cell surface glycoprotein CD44; chondroitin sulfate proteoglycan 8; CSPG8; ECMR-III; epican; Extracellular matrix receptor III; GP90 lymphocyte homing/adhesion receptor; HCAM; HCELL; hematopoietic cell E- and L-selectin ligand; Heparan sulfate proteoglycan; Hermes antigen; homing function and Indian blood group system; HUTCH-I; Hyaluronate receptor; IN; LHR; MC56; MDU2; MDU2CD44 antigen (homing function and Indian blood group system); MDU3; MDU3CDW44; MIC4; MIC4MGC10468; MUTCH-I; Pgp1; PGP-1; PGP-I; Phagocytic glycoprotein 1 CD44 CDW44, CSPG8, ECMR-III, HCELL, HUTCH-I, IN, LHR, MC56, MDU2, MDU3, MIC4, Pgp1 CD44 molecule (Indian blood group) CD44 antigen|GP90 lymphocyte homing/adhesion receptor|Hermes antigen|Indian blood group antigen|cell surface glycoprotein CD44|chondroitin sulfate proteoglycan 8|epican|extracellular matrix receptor III|hematopoietic cell E- and L-selectin ligand|heparan sulfate proteoglycan|homing function and Indian blood group system|hyaluronate receptor|phagocytic glycoprotein 1|soluble CD44

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on CD44, check out the CD44 Infographic

CD44 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for CD44: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

PA1021-2 has been cited in 10 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Clinical prognostic significance of cancer stem cell markers in patients with papillary thyroid carcinoma

CD44 promotes the migration of bone marrow-derived mesenchymal stem cells toward glioma

Rhus coriaria L. (Sumac) Demonstrates Oncostatic Activity in the Therapeutic and Preventive Model of Breast Carcinoma

Kubatka P,Kello M,Kajo K,Samec M,Jasek K,Vybohova D,Uramova S,Liskova A,Sadlonova V,Koklesova L,Murin R,Adamkov M,Smejkal K,Svajdlenka E,Solar P,Samuel SM,Kassayova M,Kwon TK,Zubor P,Pec M,Danko J,Büsselberg D,Mojzis J.Chemopreventive and Therapeutic Efficacy of Cinnamomum zeylanicum L. Bark in Experimental Breast Carcinoma: Mechanistic In Vivo and In Vitro Analyses.Molecules.2020 Mar 19;25(6):1399.doi:10.3390/molecules25061399.PMID:32204409;PMCID:PMC7144360.
Species: Mouse,Rat
PA1021-2 usage in article: APP:IHC, SAMPLE:MAMMARY GLAND TISSUE, DILUTION:NA

Wu,H.,Jiang,X.,Li,Y.,Zhou,Y.,Zhang,T.,Zhi,P.,Gao,J.,Engineering Stem Cell Derived Biomimetic Vesicles for Versatility and Effective Targeted Delivery.Adv. Funct.Mater.2020, 30, 2006169.https://doi.org/10.1002/adfm.202006169
Species: Rat,Mouse
PA1021-2 usage in article: APP:WB, SAMPLE:MSC, DILUTION:NA

Wang,Xiaoyu & Wang,Linlin & Qi,Lili & Bian,Huimiao & Yan,Xing & Wang,Yan & Li,Xiaodong & Cho,Kenka & Wu,Guojiang & Jiang,Baohong.(2020).Protective Effects of Ginsenoside Rg1 on Acute Myocardial Infarction. Journal of Pharmacy and Pharmacology Research.04.
Species: Rat
PA1021-2 usage in article: APP:IHC, SAMPLE:HEART TISSUE, DILUTION:1:100

Zhang GF,Wu JC,Wang HY,Jiang WD,Qiu L. Overexpression of microRNA-205-5p exerts suppressive effects on stem cell drug resistance in gallbladder cancer by down-regulating PRKCE. Biosci Rep.2020 Sep 30;40(9): BSR20194509.doi:10.1042/BSR20194509.PMID: 328698
Species: Human,Mouse
PA1021-2 usage in article: APP:FLOW CYTOMETRY, SAMPLE:GBC STEM CELLS, DILUTION:NA

Kubatka P,Kello M,Kajo K,Samec M,Liskova A,Jasek K,Koklesova L,Kuruc T,Adamkov M,Smejkal K,Svajdlenka E,Solar P,Pec M,Büsselberg D,Sadlonova V,Mojzis J.Rhus coriaria L.(Sumac) Demonstrates Oncostatic Activity in the Therapeutic and Preventive Model of Bre
Species: Human,Mouse,Rat
PA1021-2 usage in article: APP:IHC, SAMPLE:MAMMARY TISSUE, DILUTION:NA

Antineoplastic effects of clove buds (Syzygium aromaticum L.) in the model of breast carcinoma

Rat nasal respiratory mucosa-derived ectomesenchymal stem cells differentiate into Schwann-like cells promoting the differentiation of PC12 cells and %u2026

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16 Customer Q&As for Anti-CD44 antigen CD44 Antibody

Question

I have a question about product PA1021-2, anti-CD44 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2020-04-14

Answer

We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1021-2 anti-CD44 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2020-04-14

Question

I was wanting to use your anti-CD44 antibody for ICC for rat spinal cord on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat spinal cord identification?

Verified Customer

Verified customer

Asked: 2020-02-27

Answer

As indicated on the product datasheet, PA1021-2 anti-CD44 antibody has been tested for IHC, ICC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat spinal cord in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2020-02-27

Question

We have been able to see staining in rat foreskin. Do you have any suggestions? Is anti-CD44 antibody supposed to stain foreskin positively?

Verified Customer

Verified customer

Asked: 2020-01-14

Answer

According to literature foreskin does express CD44. According to Uniprot.org, CD44 is expressed in parotid gland, reticulocyte, myeloid leukemia cell, keratinocyte, lymphoblast, mammary carcinoma, articular cartilage, colon adenocarcinoma retinal pigment epithelium, spinal cord, pancreas retinal pigment epithelium, glial tumor, peripheral blood, foreskin, lung, plasma, cervix carcinoma, t-cell, liver, leukemic t-cell, among other tissues. Regarding which tissues have CD44 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 18691976, 20068231, 23186163
Foreskin, Pubmed ID: 2007624
Glial tumor, Pubmed ID: 7527301
Keratinocyte, Pubmed ID: 1281868
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 19159218, 24275569
Lung, Pubmed ID: 1717145, 7528188
Lymphoblast, Pubmed ID: 1465456, 1922057
Mammary carcinoma, Pubmed ID: 8352881
Myeloid leukemia cell, Pubmed ID: 2056274
Pancreas, and Retinal pigment epithelium, Pubmed ID: 15489334
Peripheral blood, Pubmed ID: 7508992
Plasma, Pubmed ID: 16335952
Reticulocyte, Pubmed ID: 1840487
Spinal cord, Pubmed ID: 17974005
T-cell, Pubmed ID: 19367720

Boster Scientific Support

Answered: 2020-01-14

Question

My boss were happy with the WB result of your anti-CD44 antibody. However we have observed positive staining in cervix carcinoma cell membrane using this antibody. Is that expected? Could you tell me where is CD44 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2020-01-06

Answer

From what I have seen in literature, cervix carcinoma does express CD44. Generally CD44 expresses in cell membrane. Regarding which tissues have CD44 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 18691976, 20068231, 23186163
Foreskin, Pubmed ID: 2007624
Glial tumor, Pubmed ID: 7527301
Keratinocyte, Pubmed ID: 1281868
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 19159218, 24275569
Lung, Pubmed ID: 1717145, 7528188
Lymphoblast, Pubmed ID: 1465456, 1922057
Mammary carcinoma, Pubmed ID: 8352881
Myeloid leukemia cell, Pubmed ID: 2056274
Pancreas, and Retinal pigment epithelium, Pubmed ID: 15489334
Peripheral blood, Pubmed ID: 7508992
Plasma, Pubmed ID: 16335952
Reticulocyte, Pubmed ID: 1840487
Spinal cord, Pubmed ID: 17974005
T-cell, Pubmed ID: 19367720

Boster Scientific Support

Answered: 2020-01-06

Question

I was wanting to use to test anti-CD44 antibody PA1021-2 on rat spinal cord for research purposes, then I may be interested in using anti-CD44 antibody PA1021-2 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-08-29

Answer

The products we sell, including anti-CD44 antibody PA1021-2, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-08-29

Question

We are currently using anti-CD44 antibody PA1021-2 for rat tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on pig tissues as well?

Verified Customer

Verified customer

Asked: 2019-08-02

Answer

The anti-CD44 antibody (PA1021-2) has not been tested for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-08-02

Question

I am interested in using your anti-CD44 antibody for t cell activation studies. Has this antibody been tested with western blotting on hela whole cell lysate? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2019-07-17

Answer

We appreciate your inquiry. This PA1021-2 anti-CD44 antibody is validated on intestinal cancer tissue, tissue lysate, rat ovary tissue, testis tissue, hela whole cell lysate, hepa whole cell lysate. It is guaranteed to work for IHC, ICC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2019-07-17

Question

Is a blocking peptide available for product anti-CD44 antibody (PA1021-2)?

Verified Customer

Verified customer

Asked: 2019-04-17

Answer

We do provide the blocking peptide for product anti-CD44 antibody (PA1021-2). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-04-17

Question

I see that the anti-CD44 antibody PA1021-2 works with ICC, what is the protocol used to produce the result images on the product page?

J. Wu

Verified customer

Asked: 2019-01-09

Answer

You can find protocols for ICC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-01-09

Question

Would anti-CD44 antibody PA1021-2 work for ICC with spinal cord?

A. Li

Verified customer

Asked: 2018-04-17

Answer

According to the expression profile of spinal cord, CD44 is highly expressed in spinal cord. So, it is likely that anti-CD44 antibody PA1021-2 will work for ICC with spinal cord.

Boster Scientific Support

Answered: 2018-04-17

Question

See below the WB image, lot number and protocol we used for spinal cord using anti-CD44 antibody PA1021-2. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2017-11-17

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2017-11-17

Question

Is this PA1021-2 anti-CD44 antibody reactive to the isotypes of CD44?

Verified Customer

Verified customer

Asked: 2017-07-28

Answer

The immunogen of PA1021-2 anti-CD44 antibody is A synthetic peptide corresponding to a sequence at the C-terminus of human CD44(728-742aa DETRNLQNVDMKIGV), different from the related mouse and rat sequences by one amino acid. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2017-07-28

Question

We ordered your anti-CD44 antibody for IHC on t-cell in the past. I am using mouse, and We are going to use the antibody for ICC next. I was wanting to use examining t-cell as well as lymphoblast in our next experiment. Do you have any suggestion on which antibody would work the best for ICC?

Verified Customer

Verified customer

Asked: 2017-07-28

Answer

I have checked the website and datasheets of our anti-CD44 antibody and it seems that PA1021-2 has been validated on mouse in both IHC and ICC. Thus PA1021-2 should work for your application. Our Boster satisfaction guarantee will cover this product for ICC in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for ICC detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2017-07-28

Question

Will PA1021-2 anti-CD44 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2017-05-23

Answer

As indicated on the product datasheet, PA1021-2 anti-CD44 antibody as been validated on ICC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2017-05-23

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for spinal cord using anti-CD44 antibody PA1021-2. Let me know if you need anything else.

A. Mitchell

Verified customer

Asked: 2016-01-12

Answer

I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2016-01-12

Question

Do you have a BSA free version of anti-CD44 antibody PA1021-2 available?

R. Kulkarni

Verified customer

Asked: 2014-02-11

Answer

I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-CD44 antibody PA1021-2 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2014-02-11

Order DetailsPrice
PA1021-2

100μg

$370
PA1021-2-10ug

10μg sample (liquid)

$99
PA1021-2-Biotin

100 μg Biotin conjugated

$570
PA1021-2-Cy3

100 μg Cy3 conjugated

$570
PA1021-2-Dylight488

100 μg Dylight488 conjugated

$570
PA1021-2-Dylight550

100 μg Dylight550 conjugated

$570
PA1021-2-Dylight594

100 μg Dylight594 conjugated

$570
PA1021-2-FITC

100 μg FITC conjugated

$570
PA1021-2-HRP

100 μg HRP conjugated

$570
PA1021-2-APC

100 μg APC conjugated

$670
PA1021-2-PE

100 μg PE conjugated

$670
PA1021-2-carrier-free

Carrier Free

$370

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PA1021-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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