|Application:||IHC-P, ICC, WB|
Data & Images
|Product Name||Anti-CD45 Antibody|
|Description||Rabbit IgG polyclonal antibody for Receptor-type tyrosine-protein phosphatase C(PTPRC) detection. Tested with WB, IHC-P, ICC in Human.|
|Cite This Product||Anti-CD45 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1596)|
|Replacement Item||This antibody may replace the following items: sc-1123|sc-1178|sc-1183|sc-1187|sc-12951-R|sc-18901|sc-19664|sc-19665|sc-20056|sc-20057|sc-25590|sc-28369|sc-51555|sc-52386|sc-52490|sc-53201|sc-53203|sc-59070|sc-59071|sc-65344|sc-66202|sc-70699|sc-70704|sc-70712 from Santa Cruz Biotechnology.|
|Application||IHC-P, ICC, WB
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P) and ICC.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human CD45(1209-1227aa MVSTFEQYQFLYDVIASTY).|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Receptor-type tyrosine-protein phosphatase C|
|Molecular Weight||147254 MW|
|Protein Function||Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity (By similarity). .|
|Sequence Similarities||Belongs to the protein-tyrosine phosphatase family. Receptor class 1/6 subfamily.|
|Subcellular Localization||Membrane ; Single-pass type I membrane protein . Membrane raft . Colocalized with DPP4 in membrane rafts.|
|Alternative Names||Receptor-type tyrosine-protein phosphatase C;126.96.36.199;Leukocyte common antigen;L-CA;T200;CD45;PTPRC;CD45;|
|Research Areas|||signal transduction|protein phosphorylation|tyrosine phosphatases| signal transduction|adapters|transmembrane||
Background for Receptor-type tyrosine-protein phosphatase C
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-CD45 Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunocytochemistry , 0.5-1μg/ml, Human, -|
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-CD45 Antibody Images
Click the images to enlarge.
All lanes: Anti CD45 (PA1596) at 0.5ug/ml
Lane 1: JURKAT Whole Cell Lysate at 40ug
Lane 2: CEM Whole Cell Lysate at 40ug
Predicted bind size: 147KD
Observed bind size: 147KD
IHC(P): Human Tonsil Tissue
ICC: JURKAT Cell
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,