|Validated Species:||Human, Rat|
Data & Images
|Product Name||Anti-CD47 Antibody|
|Description||Rabbit IgG polyclonal antibody for Leukocyte surface antigen CD47(CD47) detection. Tested with WB, IHC-P in Human;Rat.|
|Cite This Product||Anti-CD47 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2223)|
|Replacement Item||This antibody may replace the following items: sc-12731|sc-7059|sc-25773|sc-21786|sc-7057|sc-59079|sc-12730|sc-53050|sc-70716 from Santa Cruz Biotechnology.|
|Validated Species||Human, Rat|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2018!
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human CD47(308-323aa EPLNAFKESKGMMNDE), identical to the related rat sequence.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.|
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Leukocyte surface antigen CD47|
|Molecular Weight||35214 MW|
|Protein Function||Has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. Plays an important role in memory formation and synaptic plasticity in the hippocampus (By similarity). Receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation. May play a role in membrane transport and/or integrin dependent signal transduction. May prevent premature elimination of red blood cells. May be involved in membrane permeability changes induced following virus infection. .|
|Tissue Specificity||Very broadly distributed on normal adult tissues, as well as ovarian tumors, being especially abundant in some epithelia and the brain.|
|Subcellular Localization||Cell membrane ; Multi-pass membrane protein .|
|Alternative Names||Leukocyte surface antigen CD47;Antigenic surface determinant protein OA3;Integrin-associated protein;IAP;Protein MER6;CD47;CD47;MER6;|
|Research Areas|||immunology|cell type markers|adhesion| cancer|tumor immunology|cd markers||
Background for Leukocyte surface antigen CD47
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-CD47 Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, By Heat|
Western blot, 0.1-0.5μg/ml, Rat, Human
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-CD47 Antibody Images
Click the images to enlarge.
IHC(P): Human Placenta Tissue
All lanes: Anti CD47 (PA2223) at 0.5ug/ml
WB: Rat Brain Tissue Lysate at 50ug
Predicted bind size: 35KD
Observed bind size: 35KD
- Review by Peter J. Thompson
Source: Customer Feedback Submission
"This antibody gives strong membrane staining"
--Peter J. Thompson, UCSF Diabetes Center, Postdoctoral Scholar
Applications IHC-Paraffin, IHC-cryo Sample NOD/ShiLtJ mouse pancreas sections, formalin-fixed paraffin embedded Detection Zeiss Apotome widefield fluorescence microscope
"CD47 is broadly expressed in many cell types. This antibody gives strong membrane staining throughout many of the beta cells (Insulin-positive) in the immune cell infiltrated islets of NOD mice at age 12 weeks."
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,