Product Info Summary
SKU: | M00184 |
---|---|
Size: | 100ug/vial |
Reactive Species: | Human, Rat, Zebrafish |
Host: | Mouse |
Application: | Flow Cytometry, IF, IHC |
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Product info
Product Name
Anti-CD56 / NCAM1 (Neuronal Cell Marker) Monoclonal Antibody
View all NCAM-1/CD56 Antibodies
SKU/Catalog Number
M00184
Size
100ug/vial
Form
Liquid
Description
Boster Bio Anti-CD56 / NCAM1 (Neuronal Cell Marker) Monoclonal Antibody (Catalog # M00184). Tested in Flow Cytometry, IF, IHC applications. This antibody reacts with Human, Rat, Zebrafish.
Storage & Handling
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Cite This Product
Anti-CD56 / NCAM1 (Neuronal Cell Marker) Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00184)
Host
Mouse
Contents
Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Clonality
Monoclonal
Clone Number
Clone: SPM128
Isotype
IgG1, kappa
Immunogen
Membrane preparation of a small cell lung carcinoma
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00184 is reactive to NCAM1 in Human, Rat, Zebrafish
Applications
M00184 is guaranteed for Flow Cytometry, IF, IHC Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
94.574kDa
Background of NCAM-1/CD56
This monoclonal antibody reacts with an extracellular domain (close to transmembrane) of CD56/NCAM. Three isoforms of neural cell adhesion molecule (NCAM) are produced by differential splicing of the RNA transcript from a single gene. The 135kDa isoform is the basic molecule, which is glycosylated or sialylated to produce the mature species. Anti-CD56 recognizes two proteins of the neural cell adhesion molecule, the basic molecule expressed on most neuroectodermally derived tissues and neoplasms (e.g. retinoblastoma, medulloblastomas, astrocytomas, neuroblastomas, and small cell carcinomas). It is also expressed on some mesodermally derived tumors (rhabdomyosarcoma). Anti-CD56 plays an important role in the diagnosis of nodal and nasal NK/T-cell lymphomas.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 100 µL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.
Validation Images & Assay Conditions
Click image to see more details
FACS analysis of CD56 on human Monocytes using Anti-CD56 Monoclonal Antibody (SPM128)
Click image to see more details
Formalin-fixed, paraffin-embedded human Pancreas stained with Anti-CD56 Monoclonal Antibody (SPM128)
Protein Target Info & Infographic
Gene/Protein Information For NCAM1 (Source: Uniprot.org, NCBI)
Gene Name
NCAM1
Full Name
Neural cell adhesion molecule 1
Weight
94.574kDa
Alternative Names
CD56 / NCAM-1; CD56 antigen; CD56; MSK39; NCAM1; N-CAM-1; NCAM-1; NCAMantigen recognized by monoclonal 5.1H11; neural cell adhesion molecule 1; neural cell adhesion molecule, NCAM NCAM1 CD56, MSK39, NCAM neural cell adhesion molecule 1 neural cell adhesion molecule 1|antigen recognized by monoclonal antibody 5.1H11|neural cell adhesion molecule, NCAM
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NCAM1, check out the NCAM1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NCAM1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CD56 / NCAM1 (Neuronal Cell Marker) Monoclonal Antibody (M00184)
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No publications found for M00184
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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14 Customer Q&As for Anti-CD56 / NCAM1 (Neuronal Cell Marker) Monoclonal Antibody
Question
My question regarding product M00184, anti-CD56 / NCAM1 (Neuronal Cell Marker) Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-04-16
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00184 anti-CD56 / NCAM1 (Neuronal Cell Marker) Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-04-16
Question
I was wanting to use your anti-CD56 / NCAM1 (Neuronal Cell Marker) Monoclonal antibody for Flow Cytometry for human brain on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human brain identification?
Verified Customer
Verified customer
Asked: 2020-03-09
Answer
You can see on the product datasheet, M00184 anti-CD56 / NCAM1 (Neuronal Cell Marker) Monoclonal antibody has been validated for Flow Cytometry on human, rat, zebrafish tissues. We have an innovator award program that if you test this antibody and show it works in human brain in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-03-09
Question
Please see the WB image, lot number and protocol we used for brain using anti-CD56 / NCAM1 (Neuronal Cell Marker) Monoclonal antibody M00184. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-02-25
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-02-25
Question
We are currently using anti-CD56 / NCAM1 (Neuronal Cell Marker) Monoclonal antibody M00184 for rat tissue, and we are satisfied with the Flow Cytometry results. The species of reactivity given in the datasheet says human, rat, zebrafish. Is it possible that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2019-10-23
Answer
The anti-CD56 / NCAM1 (Neuronal Cell Marker) Monoclonal antibody (M00184) has not been validated for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-10-23
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain using anti-CD56 / NCAM1 (Neuronal Cell Marker) Monoclonal antibody M00184. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-07-17
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-07-17
Question
Is this M00184 anti-CD56 / NCAM1 (Neuronal Cell Marker) Monoclonal antibody reactive to the isotypes of NCAM1?
Verified Customer
Verified customer
Asked: 2019-06-21
Answer
The immunogen of M00184 anti-CD56 / NCAM1 (Neuronal Cell Marker) Monoclonal antibody is Membrane preparation of a small cell lung carcinoma. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-06-21
Question
Is there a BSA free version of anti-CD56 / NCAM1 (Neuronal Cell Marker) Monoclonal antibody M00184 available?
Verified Customer
Verified customer
Asked: 2019-04-01
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-CD56 / NCAM1 (Neuronal Cell Marker) Monoclonal antibody M00184 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-04-01
Question
My team were well pleased with the WB result of your anti-CD56 / NCAM1 (Neuronal Cell Marker) Monoclonal antibody. However we have seen positive staining in plasma isoform 1: cell membrane using this antibody. Is that expected? Could you tell me where is NCAM1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2018-11-30
Answer
Based on literature, plasma does express NCAM1. Generally NCAM1 expresses in isoform 1: cell membrane. Regarding which tissues have NCAM1 expression, here are a few articles citing expression in various tissues:
Brain, Kidney, and Ovary, Pubmed ID: 15489334
Liver, Pubmed ID: 24275569
Plasma, Pubmed ID: 16335952
Skeletal muscle, Pubmed ID: 2887295, 3253057
Boster Scientific Support
Answered: 2018-11-30
Question
Does anti-CD56 / NCAM1 (Neuronal Cell Marker) Monoclonal antibody M00184 work for Flow Cytometry with brain?
Verified Customer
Verified customer
Asked: 2018-06-22
Answer
According to the expression profile of brain, NCAM1 is highly expressed in brain. So, it is likely that anti-CD56 / NCAM1 (Neuronal Cell Marker) Monoclonal antibody M00184 will work for Flow Cytometry with brain.
Boster Scientific Support
Answered: 2018-06-22
Question
Will M00184 anti-CD56 / NCAM1 (Neuronal Cell Marker) Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-06-19
Answer
It shows on the product datasheet, M00184 anti-CD56 / NCAM1 (Neuronal Cell Marker) Monoclonal antibody as been validated on Flow Cytometry. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-06-19
Question
Is a blocking peptide available for product anti-CD56 / NCAM1 (Neuronal Cell Marker) Monoclonal antibody (M00184)?
Verified Customer
Verified customer
Asked: 2018-03-20
Answer
We do provide the blocking peptide for product anti-CD56 / NCAM1 (Neuronal Cell Marker) Monoclonal antibody (M00184). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-03-20
Question
I see that the anti-CD56 / NCAM1 (Neuronal Cell Marker) Monoclonal antibody M00184 works with Flow Cytometry, what is the protocol used to produce the result images on the product page?
K. Li
Verified customer
Asked: 2017-10-10
Answer
You can find protocols for Flow Cytometry on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2017-10-10
Question
My lab would like to test anti-CD56 / NCAM1 (Neuronal Cell Marker) Monoclonal antibody M00184 on human brain for research purposes, then I may be interested in using anti-CD56 / NCAM1 (Neuronal Cell Marker) Monoclonal antibody M00184 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2017-09-19
Answer
The products we sell, including anti-CD56 / NCAM1 (Neuronal Cell Marker) Monoclonal antibody M00184, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-09-19
Question
We have observed staining in rat testis. Do you have any suggestions? Is anti-CD56 / NCAM1 (Neuronal Cell Marker) Monoclonal antibody supposed to stain testis positively?
P. Parker
Verified customer
Asked: 2017-04-04
Answer
From what I have seen in literature testis does express NCAM1. From what I have seen in Uniprot.org, NCAM1 is expressed in neocortex, skeletal muscle, testis, brain, brain, kidney ovary, plasma, liver, among other tissues. Regarding which tissues have NCAM1 expression, here are a few articles citing expression in various tissues:
Brain, Kidney, and Ovary, Pubmed ID: 15489334
Liver, Pubmed ID: 24275569
Plasma, Pubmed ID: 16335952
Skeletal muscle, Pubmed ID: 2887295, 3253057
Boster Scientific Support
Answered: 2017-04-04