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SKU:PA1518
Clonality:Polyclonal
Application:IHC-P, IHC-F, WB
Price: $200.00
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Overview

Product Name Anti-CD68 Antibody
Description Rabbit IgG polyclonal antibody for Macrosialin(CD68) detection. Tested with WB, IHC-P, IHC-F in Mouse;Rat.
Cite This Product Anti-CD68 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1518)
Replacement Item This antibody may replace the following items: sc-5474|sc-7084|sc-59103|sc-70760|sc-52998|sc-7083|sc-20060|sc-9139|sc-17832|sc-7082|sc-70761|sc-393951|sc-514937 from Santa Cruz Biotechnology.
Host Rabbit
Isotype N/A
Validated Species Mouse, Rat
Predicted Species Hamster

*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.

Application IHC-P, IHC-F, WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P) and IHC(F).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen A synthetic peptide corresponding to a sequence in the middle region of mouse CD68(312-326aa AFCITRRRQSTYQPL), different from the related rat sequence by one amino acid.
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Polyclonal
Form Lyophilized
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
Concentration Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Immunogen affinity purified.
Isotype N/A

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name Cd68
Protein Name Macrosialin
Molecular Weight 34818 MW
Protein Function Could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells.
Tissue Specificity Expressed in tissue macrophages and to a lesser extent in dendritic cells.
Sequence Similarities Belongs to the LAMP family.
Subcellular Localization Isoform Long: Endosome membrane; Single-pass type I membrane protein. Lysosome membrane; Single-pass type I membrane protein.
Uniprot ID P31996
Alternative Names Macrosialin;CD68;Cd68;
Research Areas |immunology|cell type markers|myeloid cells|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Macrosialin

CD68, cluster of differentiation, is a 110-kD transmembrane glycoprotein that is highly expressed by human monocytes and tissue macrophages. CD68 is a member of a family of hematopoietic mucin-like molecules that includes leukosialin/CD43 and stem cell antigen CD34. The CD68 gene is mapped to 17p13.1. Immunohistochemistry can be used to identify the presence of CD68, which is found in the cytoplasmic granules of a range of different blood cells. It is particularly useful as a marker for the various cells of the macrophage lineage, including monocytes, histiocytes, giant cells, Kupffer cells, and osteoclasts. This allows it to be used to distinguish diseases of otherwise similar appearance, such as the monocyte/macrophage and lymphoid forms of leukaemia(the latter being CD68 negative). Its presence in macrophages also makes it useful in diagnosing conditions related to proliferation or abnormality of these cells, such as malignant histiocytosis, histiocytic lymphoma, and Gaucher's disease.

Anti-CD68 Antibody Images

Click the images to enlarge.

Anti-CD68 Antibody
Anti-CD68 antibody, PA1518, Western blotting
Lane 1: Rat Spleen Tissue Lysate
Lane 2: Mouse Spleen Tissue Lysate
Lane 3: Mouse RAW246.7 Tissue Lysate
Anti-CD68 Antibody
Anti-CD68 antibody, PA1518, IHC(P)
IHC(P): Rat Liver Tissue
Anti-CD68 Antibody
Anti-CD68 antibody, PA1518, IHC(P)
IHC(P): Mouse Spleen Tissue
Anti-CD68 Antibody
Anti-CD68 antibody, PA1518, IHC(F)
IHC(F): Rat Liver Tissue
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Publications

Zhou, X., Zhang, Y., Shi, X., & Fan, D. (2017). Surface Modification of Silicone Rubber by Ion Implantation to Improve Biocompatibility. In Ion Implantation – Research and Application (Chapter 6). doi: 10.5772/intechopen.68298
Guo, X., Liao, J., Huang, X., Wang, Y., Huang, W., & Liu, G. (2017). Reversal of adipose tissue loss by probucol in mice with deficiency of both scavenger receptor class B type 1 and LDL receptor on high fat diet. Biochemical and Biophysical Research Communications. Advance online publication. doi: 10.1016/j.bbrc.2017.05.017
Gu X, Wu G, Yao Y, Zeng J, Shi D, Lv T, Luo L, Song Y. Free Radic Biol Med. 2015 Jun;83:149-58. Doi: 10.1016/J.Freeradbiomed.2015.02.034. Epub 2015 Mar 13. Intratracheal Administration Of Mitochondrial Dna Directly Provokes Lung Inflammation Throu...
Wang W, Li X, Zheng D, Zhang D, Peng X, Zhang X, Ai F, Wang X, Ma J, Xiong W, Li G, Zhou Y, Shen S. Mol Med Rep. 2015 Apr;11(4):2397-406. Doi: 10.3892/Mmr.2014.3018. Epub 2014 Nov 28. Dynamic Changes And Functions Of Macrophages And M1/M2 Subpopul...
Pan X, Wang P, Luo J, Wang Z, Song Y, Ye J, Hou X. Endocrine. 2015 Apr;48(3):834-47. Doi: 10.1007/S12020-014-0384-X. Epub 2014 Aug 20. Adipogenic Changes Of Hepatocytes In A High-Fat Diet-Induced Fatty Liver Mice Model And Non-Alcoholic Fatty Live...
Zhang Jx, Wu Hs, Wang H, Zhang Jh, Wang Y, Zheng Qc. World J Gastroenterol. 2005 Jul 28;11(28):4423-6. Protection Against Hepatic Ischemia/Reperfusion Injury Via Downregulation Of Toll-Like Receptor 2 Expression By Inhibition Of Kupffer Cell Funct...
Liu B, Luo Xj, Yang Zb, Zhang Jj, Li Tb, Zhang Xj, Ma Ql, Zhang Gg, Hu Cp, Peng J. J Cardiovasc Pharmacol. 2014 Jun;63(6):567-76. Doi: 10.1097/Fjc.0000000000000082. Inhibition Of Nox/Vpo1 Pathway And Inflammatory Reaction By Trimethoxystilbene In ...
Zheng T,, Yang X,, Wu D,, Xing S,, Bian F,, Li W,, Chi J,, Bai X,, Wu G,, Chen X,, Zhang Y,, Jin S,,. Br J Pharmacol. 2015 Jul;172(13):3284-301. Doi: 10.1111/Bph.13120. Epub 2015 Apr 24. Salidroside Ameliorates Insulin Resistance Through Activatio...
He W, Lv Y, Zhao Y, Xu C, Jin Z, Qin C, Yin L. Int J Pharm. 2015 Apr 30;484(1-2):163-71. Doi: 10.1016/J.Ijpharm.2015.02.053. Epub 2015 Feb 24. Core-Shell Structured Gel-Nanocarriers For Sustained Drug Release And Enhanced Antitumor Effect.
Liu Z,, Xu S,, Huang X, Wang J, Gao S, Li H, Zhou C, Ye J, Chen S, Jin Zg, Liu P. Br J Pharmacol. 2015 Dec;172(23):5661-75. Doi: 10.1111/Bph.13068. Epub 2015 Feb 27. Cryptotanshinone, An Orally Bioactive Herbal Compound From Danshen, Attenuates At...
Peng K, Liu L, Wei D, Lv Y, Wang G, Xiong W, Wang X, Altaf A, Wang L, He D, Wang H, Qu P. Int J Mol Med. 2015 May;35(5):1179-88. Doi: 10.3892/Ijmm.2015.2129. Epub 2015 Mar 9. P2X7R Is Involved In The Progression Of Atherosclerosis By Promoting Nlr...
Xiang S, Liu J, Dong N, Shi J, Xiao Y, Wang Y, Hu X, Gong L, Wang W. J Vasc Res. 2014;51(2):132-43. Doi: 10.1159/000355193. Epub 2014 Mar 28. Suppressor Of Cytokine Signaling 3 Is A Negative Regulator For Neointimal Hyperplasia Of Vein Graft Steno...

FAQs

Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: Has this antibody been tested for cross-reactivity with human samples in IHC or Western Blot?
A: We tested human samples and got negative results in IHC and WB.
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
A: You can find the immunogen sequence under "Immunogen" and clonality in the product name.
Q: What is the expected band size? Why is it different than the observed band size?
A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands

3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.

4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
A: Check our protocols under the tech support tab.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to cd68 antibody, cd 68 antibody
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to cd68 antibody, cd 68 antibody