|Application:||IHC-P, ICC, WB|
Data & Images
|Product Name||Anti-CD79b Picoband™ Antibody|
|Description||Rabbit IgG polyclonal antibody for B-cell antigen receptor complex-associated protein beta chain(CD79B) detection. Tested with WB, IHC-P, ICC in Human;Mouse;Rat.|
|Cite This Product||Anti-CD79b Picoband™ Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9169)|
|Replacement Item||This antibody may replace the following items: sc-25605|sc-53210|sc-13530|sc-8506|sc-80538|sc-18902|sc-8504|sc-59116|sc-373843|sc-166675|sc-8505|sc-373808|sc-59115|sc-59114 from Santa Cruz Biotechnology.|
|Validated Species||Human, Mouse, Rat|
|Application||IHC-P, ICC, WB
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P) and ICC.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||E.coli-derived human CD79b recombinant protein (Position: A29-E229). Human CD79b shares 70% amino acid (aa) sequence identity with mouse CD79b.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
*carrier free antibody available upon request.
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||B-cell antigen receptor complex-associated protein beta chain|
|Molecular Weight||26048 MW|
|Protein Function||Required in cooperation with CD79A for initiation of the signal transduction cascade activated by the B-cell antigen receptor complex (BCR) which leads to internalization of the complex, trafficking to late endosomes and antigen presentation. Enhances phosphorylation of CD79A, possibly by recruiting kinases which phosphorylate CD79A or by recruiting proteins which bind to CD79A and protect it from dephosphorylation. .|
|Sequence Similarities||Contains 1 Ig-like V-type (immunoglobulin-like) domain.|
|Subcellular Localization||Cell membrane; Single-pass type I membrane protein. Following antigen binding, the BCR has been shown to translocate from detergent-soluble regions of the cell membrane to lipid rafts although signal transduction through the complex can also occur outside lipid rafts. .|
|Alternative Names||B-cell antigen receptor complex-associated protein beta chain;B-cell-specific glycoprotein B29;Ig-beta;Immunoglobulin-associated B29 protein;CD79b;CD79B;B29, IGB;|
|Research Areas|||immunology|adaptive immunity|b cells||
Background for B-cell antigen receptor complex-associated protein beta chain
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-CD79b Picoband™ Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunohistochemistry(Frozen Section), 0.5-1μg/ml, Human, -|
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Western blot, 0.1-0.5μg/ml, Human
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-CD79b Picoband™ Antibody Images
Click the images to enlarge.
All lanes: Anti CD79B (PB9169) at 0.5ug/ml
WB: Recombinant Human CD79B Protein 0.5ng
Predicted bind size: 45KD
Observed bind size: 45KD
All lanes: Anti CD79b (PB9169) at 0.5ug/ml
Lane 1: Raji Whole Cell Lysate at 40ug
Lane 2: Hl-60 Whole Cell Lysate at 40ug
Lane 3: HUT Whole Cell Lysate at 40ug
Predicted bind size: 26KD
Observed bind size: 35KD
IHC(P): Mouse Spleen Tissue
IHC(P): Rat Spleen Tissue
IHC(P): Human Tonsil Tissue
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,