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SKU:MA1017
Pack Size:100μg/vial
Sample Size:30ug for $99, contact us for details
Clonality:Monoclonal
Clone Number:CA-8
Application:IHC-F
Price: $200.00
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Overview

Product Name Anti-CD8 Antibody (Monoclonal, CA-8)
SKU/Catalog Number MA1017
Description Mouse IgG monoclonal antibody for CD8, CD8a molecule; CD8b molecule (CD8A; CD8B) detection. Tested with IHC-F in Human. No cross reactivity with other proteins.
Cite This Product Anti-CD8 Antibody (Monoclonal, CA-8) CA-8 (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1017)
Replacement Item This antibody may replace the following items: sc-1141|sc-1143|sc-1144|sc-1177|sc-1181|sc-19994|sc-25277|sc-51735|sc-53212|sc-70791|sc-70794|sc-7188|sc-7970|sc-9147 from Santa Cruz Biotechnology.
Host Mouse
Isotype Mouse IgG2a
Validated Species Human
Application IHC-F

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(F).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen Human thymocytes followed by peripheral blood T cells.
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Monoclonal
Clone Number CA-8
Form Lyophilized
Contents Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
*carrier free antibody available upon request.
Concentration Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Ascites
Isotype Mouse IgG2a

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name Cd8b
Protein Name T-cell surface glycoprotein CD8 alpha chain; T-cell surface glycoprotein CD8 beta chain
Molecular Weight 23400 MW
Protein Function Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing.
Sequence Similarities Contains 1 Ig-like V-type (immunoglobulin-like) domain.
Subcellular Localization Membrane; Single-pass type I membrane protein.
Uniprot ID P05541
Alternative Names T-cell surface glycoprotein CD8 beta chain;CD8 antigen 37 kDa chain;OX-8 membrane antigen;CD8b;Cd8b;Cd8b1;
Research Areas CD8A|CD8B|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for T-cell surface glycoprotein CD8 alpha chain; T-cell surface glycoprotein CD8 beta chain

The human lymphocyte differentiation antigen CD8 is encoded by a single gene that gives rise to a 33- to 34-kDa glycoprotein expressed on the cell surface as a dimer and in higher molecular mass forms.
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Publications

Xu Q, Chen Y, Zhao Wm, Huang Zy, Duan Xj, Tong Yy, Zhang Y, Li X, Chang Gb, Chen Gh. Mol Biol Rep. 2015 Feb;42(2):431-9. Doi: 10.1007/S11033-014-3784-3. Epub 2014 Oct 21. The Cd8?? Gene In Duck (Anatidae): Cloning, Characterization, And Expression ...
Zhao N, Li X, He X, Qiu Y, Zhu L, Qi F. J Gene Med. 2013 Oct;15(10):366-74. Doi: 10.1002/Jgm.2739. Interleukin-15 Gene Therapy And The Mammalian Target Of Rapamycin Inhibitor Everolimus Inhibit The Growth Of Metastatic Breast Cancer.
Wang X, Shi L, Tu Q, Wang H, Zhang H, Wang P, Zhang L, Huang Z, Zhao F, Luan H, Wang X. Int J Nanomedicine. 2015 Jan 6;10:347-55. Doi: 10.2147/Ijn.S71245. Ecollection 2015. Treating Cutaneous Squamous Cell Carcinoma Using 5-Aminolevulinic Acid Pol...
Xu Y, Tan X, Wang D, Wang W, Li Y, Wu M, Chen S, Wu Y, Tan C. Int J Mol Med. 2015 Jun;35(6):1501-10. Doi: 10.3892/Ijmm.2015.2154. Epub 2015 Mar 24. Elevated Survivin Expression In Peripheral Blood Mononuclear Cells Is Central To Collateral Formati...
Wu Y, Wang Y, Qi X, Zhang P, Zhang C, Zhang W. Nephron Exp Nephrol. 2014;128(1-2):46-56. Doi: 10.1159/000366446. Epub 2014 Nov 5. Increased Macrophage Activation Inhibited By Tacrolimus In The Kidney Of Diabetic Rats.

FAQs

Q: What would be the approximate optimal dilution for this antibody? Can we also use this antibody on paraffin embedded sections? Keyword: protocol, applications
A: The antibodies are lyophilized, and the recommended working concentration is 1μg/ml. This antibody can only be used on frozen section.
Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: Can you provide monoclonal antibodies carrier free?
A: We cannot.
Q: What are some alternative names that could be used to describe this product?
A: One other very common name is cd86 antibody