|Sample Size:||30ug for $99, contact us for details|
Data & Images
|Product Name||Anti-B7-1/CD80 Antibody|
|Description||Rabbit IgG polyclonal antibody for T-lymphocyte activation antigen CD80(CD80) detection. Tested with WB in Human.|
|Cite This Product||Anti-B7-1/CD80 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1365)|
|Replacement Item||This antibody may replace the following items: sc-11027|sc-11033|sc-11034|sc-271878|sc-11027|sc-11030|sc-11032|sc-11033|sc-11034|sc-25660|sc-271878 from Santa Cruz Biotechnology.|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||A synthetic peptide corresponding to a sequence at the N-terminus of human CD80(57-71aa EELAQTRIYWQKEKK).|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||T-lymphocyte activation antigen CD80|
|Molecular Weight||33048 MW|
|Protein Function||Involved in the costimulatory signal essential for T- lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28, binding to CTLA-4 has opposite effects and inhibits T-cell activation. .|
|Tissue Specificity||Expressed on activated B-cells, macrophages and dendritic cells.|
|Sequence Similarities||Contains 1 Ig-like C2-type (immunoglobulin-like) domain.|
|Subcellular Localization||Membrane; Single-pass type I membrane protein.|
|Alternative Names||T-lymphocyte activation antigen CD80;Activation B7-1 antigen;BB1;CTLA-4 counter-receptor B7.1;B7;CD80;CD80;CD28LG, CD28LG1, LAB7;|
|Research Areas|||immunology|adaptive immunity|b cells| stem cells|hematopoietic progenitors|lymphoid|b lymphocytic lineage|myeloid|dendritic cell lineage|monocytic lineage||
Background for T-lymphocyte activation antigen CD80
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-B7-1/CD80 Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Western blot, 0.1-0.5μg/ml, Human|
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-B7-1/CD80 Antibody Images
Click the images to enlarge.
Lane 1: Recombinant Human CD80 Protein 10ng
Lane 2: Recombinant Human CD80 Protein 5ng
Lane 3: Recombinant Human CD80 Protein 2.5ng
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,