Anti-CD80/B7 1 Rabbit Monoclonal Antibody

B7-1/CD80 antibody

Boster Bio Anti-CD80/B7 1 Rabbit Monoclonal Antibody catalog # M00196. Tested in WB, IHC applications. This antibody reacts with Human. Cited in 1 publication(s).

Product Info Summary

SKU: M00196
Size: 100 μl
Reactive Species: Human
Host: Rabbit
Application: IHC, WB

Product Name

Anti-CD80/B7 1 Rabbit Monoclonal Antibody

View all B7-1/CD80 Antibodies

SKU/Catalog Number

M00196

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-CD80/B7 1 Rabbit Monoclonal Antibody catalog # M00196. Tested in WB, IHC applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-CD80/B7 1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00196)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

AOEH-3

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human CD80

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Detects ~20kDa. Does not cross-react with αB-crystallin, βL-crystallin, ΒH- crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins.

Reactive Species

M00196 is reactive to CD80 in Human

Applications

M00196 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

103 kDa

Calculated molecular weight

33.048kDa

Background of B7-1/CD80

C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Innovating Scientists Reward

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
IHC 1:50-1:200

Validation Images & Assay Conditions

Gene/Protein Information For CD80 (Source: Uniprot.org, NCBI)

Gene Name

CD80

Full Name

T-lymphocyte activation antigen CD80

Weight

33.048kDa

Alternative Names

Activation B7-1 antigen; B7; B71; B7-1; BB1; CD28LG1; CD28LGB7-1 antigen); CD80 antigen; CD80 molecule; CD80; costimulatory factor CD80; costimulatory molecule variant IgV-CD80; CTLA-4 counter-receptor B7.1; T-lymphocyte activation antigen CD80 CD80 B7, B7-1, B7.1, BB1, CD28LG, CD28LG1, LAB7 CD80 molecule T-lymphocyte activation antigen CD80|B-lymphocyte activation antigen B7|CD80 antigen (CD28 antigen ligand 1, B7-1 antigen)|CTLA-4 counter-receptor B7.1|activation B7-1 antigen|costimulatory factor CD80|costimulatory molecule variant IgV-CD80

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on CD80, check out the CD80 Infographic

CD80 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for CD80: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

M00196 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Chen X,Gao A,Zhang F,Yang Z,Wang S,Fang Y,Li J,Wang J,Shi W,Wang L, Zheng Y,Sun Y.ILT4 inhibition prevents TAM- and dysfunctional T cell-mediated immunosuppression and enhances the efficacy of anti-PD-L1 therapy in NSCLC with EGFR activation.Theranostics.
Species: Human,Mouse
M00196 usage in article: APP:IF, SAMPLE:TUMOR CELL LINES, DILUTION:1:100

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5 Customer Q&As for Anti-CD80/B7 1 Rabbit Monoclonal Antibody

Question

We have observed staining in human brain. Any tips? Is anti-CD80/B7 1 Rabbit Monoclonal antibody supposed to stain brain positively?

Verified Customer

Verified customer

Asked: 2020-04-30

Answer

According to literature brain does express CD80. According to Uniprot.org, CD80 is expressed in gastrocnemius, lymphoid tissue, brain, erythroleukemia, among other tissues. Regarding which tissues have CD80 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Erythroleukemia, Pubmed ID: 23186163
Lymphoid tissue, Pubmed ID: 2794510, 1377173

Boster Scientific Support

Answered: 2020-04-30

Question

I was wanting to use using your anti-CD80/B7 1 Rabbit Monoclonal antibody for positive regulation of t-helper 1 cell differentiation studies. Has this antibody been tested with western blotting on raji cell lysate? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2019-10-24

Answer

Thanks for your inquiry. This M00196 anti-CD80/B7 1 Rabbit Monoclonal antibody is tested on raji cell lysate. It is guaranteed to work for IHC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2019-10-24

Question

We purchased anti-CD80/B7 1 Rabbit Monoclonal antibody for WB on gastrocnemius in the past. I am using human, and We intend to use the antibody for IHC next. I am looking for examining gastrocnemius as well as erythroleukemia in our next experiment. Could give a recommendation on which antibody would work the best for IHC?

Verified Customer

Verified customer

Asked: 2019-06-26

Answer

I looked at the website and datasheets of our anti-CD80/B7 1 Rabbit Monoclonal antibody and I see that M00196 has been tested on human in both WB and IHC. Thus M00196 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2019-06-26

Question

My boss were satisfied with the WB result of your anti-CD80/B7 1 Rabbit Monoclonal antibody. However we have observed positive staining in lymphoid tissue membrane using this antibody. Is that expected? Could you tell me where is CD80 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2018-10-26

Answer

According to literature, lymphoid tissue does express CD80. Generally CD80 expresses in membrane. Regarding which tissues have CD80 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Erythroleukemia, Pubmed ID: 23186163
Lymphoid tissue, Pubmed ID: 2794510, 1377173

Boster Scientific Support

Answered: 2018-10-26

Question

We are currently using anti-CD80/B7 1 Rabbit Monoclonal antibody M00196 for human tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on dog tissues as well?

Verified Customer

Verified customer

Asked: 2017-07-28

Answer

The anti-CD80/B7 1 Rabbit Monoclonal antibody (M00196) has not been validated for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2017-07-28

Order DetailsPrice
M00196

100μL

$370
M00196-30ul

30μL sample

$99
M00196-carrier-free

Carrier Free

$370

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