Data & Images
|Product Name||Anti-CD82 Antibody|
|Description||Rabbit IgG polyclonal antibody for CD82 antigen(CD82) detection. Tested with WB in Human;Mouse;Rat.|
|Cite This Product||Anti-CD82 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1307)|
|Replacement Item||This antibody may replace the following items: sc-1087|sc-17752|sc-5540|sc-5540-X from Santa Cruz Biotechnology.|
|Validated Species||Human, Mouse, Rat|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human CD82(253-267aa CRHVHSEDYSKVPKY), different from the related mouse and rat sequences by two amino acids.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 50% glycerol, 0.9mg NaCl, 0.2mg Na2HPO4. Carrier free (No BSA) form available in stock. If you want this antibody carrier free please specify "Carrier Free" or "No BSA" in your order note.|
|Storage||At -20˚C for one year, at 4˚C for one month. Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||CD82 antigen|
|Molecular Weight||29626 MW|
|Protein Function||Associates with CD4 or CD8 and delivers costimulatory signals for the TCR/CD3 pathway.|
|Tissue Specificity||Lymphoid specific.|
|Sequence Similarities||Belongs to the tetraspanin (TM4SF) family.|
|Subcellular Localization||Membrane; Multi-pass membrane protein.|
|Alternative Names||CD82 antigen;C33 antigen;IA4;Inducible membrane protein R2;Metastasis suppressor Kangai-1;Suppressor of tumorigenicity 6 protein;Tetraspanin-27;Tspan-27;CD82;CD82;KAI1, SAR2, ST6, TSPAN27;|
|Research Areas|||immunology|adaptive immunity|t cells| immunology|cell type markers|nk cells|platelets| epigenetics and nuclear signaling|transcription|cancer susceptibility|tumor suppressors| cancer|oncoproteins/suppressors||
Background for CD82 antigen
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-CD82 Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat|
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-CD82 Antibody Images
Click the images to enlarge.
Lane 1: JURKAT Cell Lysate
Lane 2: HMY2 Cell Lysate
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: mouse liver tissue lysates,
Lane 2: mouse thymus tissue lysates,
Lane 3: rat brain tissue lysates,
Lane 4: rat thymus tissue lysates,
Lane 5: PC-12 whole Cell lysates,
Lane 6: A375 whole Cell lysates,
Lane 7: PANC-1 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- CD82 antigen affinity purified polyclonal antibody (Catalog # PA1307) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD82 at approximately 35KD. The expected band size for CD82 is at 30KD.
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,