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SKU:PA1307
Clonality:Polyclonal
Application:WB
Price: $200.00
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Overview

Product Name Anti-CD82 Antibody
Description Rabbit IgG polyclonal antibody for CD82 antigen(CD82) detection. Tested with WB in Human;Mouse;Rat.
Cite This Product Anti-CD82 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1307)
Replacement Item This antibody may replace the following items: sc-1087|sc-17752|sc-5540|sc-5540-X from Santa Cruz Biotechnology.
Host Rabbit
Isotype N/A
Validated Species Human, Mouse, Rat
Predicted Species Hamster

*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.

Application WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen A synthetic peptide corresponding to a sequence at the C-terminus of human CD82(253-267aa CRHVHSEDYSKVPKY), different from the related mouse and rat sequences by two amino acids.
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Polyclonal
Form Liquid
Contents Each vial contains 50% glycerol, 0.9mg NaCl, 0.2mg Na2HPO4. Carrier free (No BSA) form available in stock. If you want this antibody carrier free please specify "Carrier Free" or "No BSA" in your order note.
Storage At -20˚C for one year, at 4˚C for one month. Avoid repeated freezing and thawing.
Purification Immunogen affinity purified.
Isotype N/A

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name CD82
Protein Name CD82 antigen
Molecular Weight 29626 MW
Protein Function Associates with CD4 or CD8 and delivers costimulatory signals for the TCR/CD3 pathway.
Tissue Specificity Lymphoid specific.
Sequence Similarities Belongs to the tetraspanin (TM4SF) family.
Subcellular Localization Membrane; Multi-pass membrane protein.
Uniprot ID P27701
Alternative Names CD82 antigen;C33 antigen;IA4;Inducible membrane protein R2;Metastasis suppressor Kangai-1;Suppressor of tumorigenicity 6 protein;Tetraspanin-27;Tspan-27;CD82;CD82;KAI1, SAR2, ST6, TSPAN27;
Research Areas |immunology|adaptive immunity|t cells| immunology|cell type markers|nk cells|platelets| epigenetics and nuclear signaling|transcription|cancer susceptibility|tumor suppressors| cancer|oncoproteins/suppressors|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for CD82 antigen

CD82(Cluster of Differentiation 82), also named KAI1, is a human protein encoded by the CD82 gene. The gene is mapped to 11p11.2. This metastasis suppressor gene product is a membrane glycoprotein that is a member of the transmembrane 4 superfamily. Expression of this gene has been shown to be downregulated in tumor progression of human cancers and can be activated by p53 through a consensus binding sequence in the promoter. The expression of CD82 protein appears to be correlated with lymph node metastasis in esophageal squamous cell carcinoma(ESCC). And the CD82 overexpression can suppress tumor invasiveness and metastatic potential by inducing MMP9 inactivation via upregulation of TIMP1.

Anti-CD82 Antibody Images

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Anti-CD82 Antibody
Anti-CD82 antibody, PA1307, Western blotting
Lane 1: JURKAT Cell Lysate
Lane 2: HMY2 Cell Lysate
Anti-CD82 Antibody
Figure 2. Western blot analysis of CD82 using anti- CD82 antibody (PA1307).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: mouse liver tissue lysates,
Lane 2: mouse thymus tissue lysates,
Lane 3: rat brain tissue lysates,
Lane 4: rat thymus tissue lysates,
Lane 5: PC-12 whole Cell lysates,
Lane 6: A375 whole Cell lysates,
Lane 7: PANC-1 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- CD82 antigen affinity purified polyclonal antibody (Catalog # PA1307) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD82 at approximately 35KD. The expected band size for CD82 is at 30KD.
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FAQs

Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
A: You can find the immunogen sequence under "Immunogen" and clonality in the product name.
Q: What is the expected band size? Why is it different than the observed band size?
A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands

3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.

4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
A: Check our protocols under the tech support tab.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to cd82 antibody, kai1 antibody